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Goto, Tsuyoshi,Hirata, Mariko,Aoki, Yumeko,Iwase, Mari,Takahashi, Haruya,Kim, Minji,Li, Yongjia,Jheng, Huei-Fen,Nomura, Wataru,Takahashi, Nobuyuki,Kim, Chu-Sook,Yu, Rina,Seno, Shigeto,Matsuda, Hideo,A American Society for Biochemistry and Molecular Bi 2017 The Journal of biological chemistry Vol.292 No.22
<P>Obesity causes excess fat accumulation in white adipose tissues (WAT) and also in other insulin-responsive organs such as the skeletal muscle, increasing the risk for insulin resistance, which can lead to obesity-related metabolic disorders. Peroxisome proliferator-activated receptor-alpha (PPAR alpha) is a master regulator of fatty acid oxidation whose activator is known to improve hyperlipidemia. However, the molecular mechanisms underlying PPAR alpha activator-mediated reduction in adiposity and improvement of metabolic disorders are largely unknown. In this study we investigated the effects of PPAR alpha agonist (fenofibrate) on glucose metabolism dysfunction in obese mice. Fenofibrate treatment reduced adiposity and attenuated obesity-induced dysfunctions of glucose metabolism in obese mice fed a high-fat diet. However, fenofibrate treatment did not improve glucose metabolism in lipodystrophic A-Zip/F1 mice, suggesting that adipose tissue is important for the fenofibrate-mediated amelioration of glucose metabolism, although skeletal muscle actions could not be completely excluded. Moreover, we investigated the role of the hepatokine fibroblast growth factor 21 (FGF21), which regulates energy metabolism in adipose tissue. In WAT of WT mice, but not of FGF21-deficient mice, fenofibrate enhanced the expression of genes related to brown adipocyte functions, such as Ucp1, Pgc1a, and Cpt1b. Fenofibrate increased energy expenditure and attenuated obesity, whole body insulin resistance, and adipocyte dysfunctions in WAT in high-fat-diet-fed WT mice but not in FGF21-defi-the fenofibrate-mediated improvement of whole body glucose metabolism in obese mice via the amelioration of WAT dysfunctions.</P>
Nandre, R.M.,Matsuda, K.,Chaudhari, A.A.,Kim, B.,Lee, J.H. British Veterinary Association [etc] ; W.B. Saunde 2012 Research in veterinary science Vol.93 No.2
To construct a novel live Salmonella Enteritidis (SE) vaccine candidate, SE was genetically engineered using the allelic exchange method to delete two virulence genes, lon and cpxR. The lon gene deletion is essential to impair Salmonella replication and avoid overwhelming systemic disease in the host. The cpxR gene deletion is needed to enhance the ability of bacteria to adhere and invade the host cell. Scanning electron microscopy revealed that the derivatives JOL917 (Δlon), JOL918 (ΔcpxR), and JOL919 (Δlon/ΔcpxR) had increased surface fimbrial filamentous structures. Significant elevations of extracellular polysaccharide and FimA expression were observed for the derivatives compared to the parental wild type JOL860, while biochemical properties of the derivatives were not altered. In the safety examination by inoculation of the derivatives in chickens, gross lesion scores of the liver, spleen, kidney, small intestine and caecal tonsils were moderate in the JOL917 and JOL918 groups, and significantly lower in the JOL919 group than those of the JOL860. Bacterial counts from the spleen and caeca of the JOL917 and JOL918 groups were moderate, and significantly reduced in the JOL919 group compared to the JOL860 group. In addition, only the JOL919 group showed significantly lower bacterial counts in the faecal samples than those of the JOL860 group. Significant elevations of IgG and secretory IgA levels observed in the derivative groups, while the JOL919 and JOL860 groups showed a potent lymphocyte proliferation response as compared to those of the control group. In the protection efficacy examination, JOL919 immunized group showed significantly lower depression, lower gross lesion in the liver and spleen, and lower number of the SE positive internal organs than those of the control group against a virulent wild type SE challenge.
Chaudhari, Atul A,Matsuda, Kiku,Lee, John Hwa American Association of Avian Pathologists [etc.] 2013 Avian diseases Vol.57 No.1
<P>An attenuated Salmonella (deltalon, deltacpxR, and deltaasdA16) delivery system containing the genes encoding P-fimbriae (papa and papG), aerobactin receptor (iutA), and CS31A surface antigen (clpG) of avian pathogenic Escherichia coli (APEC) was constructed, and its potential as a vaccine candidate against APEC infection in chickens was evaluated. The birds were divided into three groups designated group A (nonvaccinated control), group B (given a single immunization), and group C (administered prime and boost immunizations). Prime and booster vaccinations with the constructions were administered to 1-day-old and 14-day-old birds, respectively. Immune responses were measured postimmunization, and the birds were challenged via an intra-air sac route with a virulent APEC strain at the second, third, and fourth weeks of age. Group B birds were partially protected against the challenge and showed increased levels of plasma immunoglobulin (Ig)G, mucosal IgA antibodies, and lymphocyte proliferation. Group C birds showed greater protection against the challenge, with significantly stronger immune responses compared with the birds in the other groups. Overall, our data suggest that the Salmonella delivery system with recombinant constructs is capable of inducing robust immune responses and induces effective protection against colibacillosis caused by APEC.</P>
Contribution of a Non-classical HLA Gene, HLA-DOA, to the Risk of Rheumatoid Arthritis
Okada, Y.,Suzuki, A.,Ikari, K.,Terao, C.,Kochi, Y.,Ohmura, K.,Higasa, K.,Akiyama, M.,Ashikawa, K.,Kanai, M.,Hirata, J.,Suita, N.,Teo, Y.Y.,Xu, H.,Bae, S.C.,Takahashi, A.,Momozawa, Y.,Matsuda, K.,Momoh University of Chicago Press [etc.] 2016 American journal of human genetics Vol.99 No.2
<P>Despite the progress in human leukocyte antigen (HLA) causal variant mapping, independent localization of major histocompatibility complex (MHC) risk from classical HLA genes is challenging. Here, we conducted a large-scale MHC fine-mapping analysis of rheumatoid arthritis (RA) in a Japanese population (6,244 RA cases and 23,731 controls) population by using HLA imputation, followed by a multi-ethnic validation study including east Asian and European populations (n=7,097 and 23,149, respectively). Our study identified an independent risk of a synonymous mutation at HLA-DOA, a non-classical HLA gene, on anti-citrullinated protein autoantibody (ACPA)-positive RA risk (p=1.4 x 10(-) 9), which demonstrated a cis-expression quantitative trait loci (cis-eQTL) effect on HLA-DOA expression. Trans-ethnic comparison revealed different linkage disequilibrium (LD) patterns in HLA-DOA and HLA-DRB1, explaining the observed HLA-DOA variant risk heterogeneity among ethnicities, which was most evident in the Japanese population. Although previous HLA fine-mapping studies have identified amino acid polymorphisms of the classical HLA genes as driving genetic susceptibility to disease, our study additionally identifies the dosage contribution of a non-classical HLA gene to disease etiology. Our study contributes to the understanding of HLA immunology in human diseases and suggests the value of incorporating additional ancestry in MHC fine-mapping.</P>
Matsuda, Kiku,Chaudhari, Atul A.,Kim, Sam Woong,Lee, Kyeong Min,Lee, John Hwa EDP Sciences 2010 VETERINARY RESEARCH Vol.41 No.5
<P>To construct a novel live vaccine candidate for fowl typhoid (FT) caused by <I>Salmonella</I> Gallinarum (SG), the <I>lon</I> and <I>cpxR</I> genes that are related to host-pathogen interaction were deleted from a wild type SG using the allelic exchange method. The mutants were grown normally, as was the wild type. The biochemical properties of the mutants remained very similar to those of the wild-type, while JOL914 (Δ<I>lon</I>) and JOL916 (Δ<I>lon</I>Δ<I>cpxR</I>) were mucoid. Extracellular polysaccharide increased 30.6-, 1.3-, and 46.2-fold in JOL914, JOL915 (Δ<I>cpxR</I>), and JOL916, respectively. Dot-blot analysis demonstrated significant increases of FimA expression at 6.77-, 2.33-, and 3.90-fold for JOL914, JOL915, and JOL916, respectively. Internalizations of JOL914, JOL915, and JOL916, in chicken abdominal macrophages, were increased at 4.65-, 0.50-, and 2.72-fold, respectively. Virulences of JOL914, JOL915 and JOL916, analyzed by LD<SUB>50</SUB> using 1-week-old chickens, were attenuated approximately at 10<SUP>1</SUP>-, 10<SUP>1</SUP>-, and > 10<SUP>3</SUP>-fold, respectively. The oral inoculations of 2 × 10<SUP>7</SUP> cfu of the wild type, JOL914, JOL915 and JOL916 caused 55.6, 16.7, 22.2, and 0.0% mortality, respectively. Significantly moderate gross lesions of the liver and spleen were observed in the JOL916 group compared to the other groups. An induced immune response and significant peripheral mononuclear proliferation reaction were observed in the JOL916 group. At the protection against the wild type challenge, JOL916 offered 100% protection. Thus, the results of this study suggest that JOL916 among the mutants studied represented the safest and most effective live vaccine candidate against FT.</P>
Masao Higo,Katsunori Isobe,Yusuke Miyazawa,Yukiya Matsuda,Rhae A. Drijber,Yoichi Torigoe 한국미생물학회 2016 The journal of microbiology Vol.54 No.2
A clear understanding of how crop root proliferation affects the distribution of the spore abundance of arbuscular mycorrhizal fungi (AMF) and the composition of AMF communities in agricultural fields is imperative to identify the potential roles of AMF in winter cover crop rotational systems. Toward this goal, we conducted a field trial using wheat (Triticum aestivum L.) or red clover (Trifolium pratense L.) grown during the winter season. We conducted a molecular analysis to compare the diversity and distribution of AMF communities in roots and spore abundance in soil cropped with wheat and red clover. The AMF spore abundance, AMF root colonization, and abundance of root length were investigated at three different distances from winter crops (0 cm, 7.5 cm, and 15 cm), and differences in these variables were found between the two crops. The distribution of specific AMF communities and variables responded to the two winter cover crops. The majority of Glomerales phylotypes were common to the roots of both winter cover crops, but Gigaspora phylotypes in Gigasporales were found only in red clover roots. These results also demonstrated that the diversity of the AMF colonizing the roots did not significantly change with the three distances from the crop within each rotation but was strongly influenced by the host crop identity. The distribution of specific AMF phylotypes responded to the presence of wheat and red clover roots, indicating that the host crop identity was much more important than the proliferation of crop roots in determining the diversity of the AMF communities.
Matsuda, Kiku,Chaudhari, Atul A.,Lee, John Hwa Elsevier 2010 Research in veterinary science Vol.89 No.2
<P><B>Abstract</B></P><P>An intestinal pathogenic <I>Escherichia coli</I> isolate from calf diarrhea, containing the <I>iutA</I>, <I>f17A</I>, <I>afa</I>-<I>8D</I>, and <I>cnf2</I> genes, was able to cause avian colibacillosis after experimental infection in chickens. Intra-tracheal inoculation and spray of this strain caused 10% of mortality and gross lesions, including airsacculitis, pericarditis, and perihepatitis. These results suggest that some bovine pathogenic <I>E. coli</I> can cause extra-intestinal infections in other animal species.</P>
Surface and Electronic Properties of Hydrogen Terminated Si [001] Nanowires
Matsuda, Yuki,Tahir-Kheli, Jamil,Goddard , William A. American Chemical Society 2011 JOURNAL OF PHYSICAL CHEMISTRY C - Vol.115 No.25
<P>The calculated band gaps reported previously for silicon nanowires (SiNW) have disagreed with the experimental values both in magnitude and in the behavior with radius. We resolve this discrepancy here. We report ab initio quantum mechanical calculations of hydrogen terminated Si [001] nanowires (H–SiNWs) as a function of diameter (<I>d</I>) and hydrogen coverage using the B3LYP density functional. For smaller diameters (<I>d</I> ≤ 1.9 nm) we find that the most stable surface is fully saturated with hydrogen leading to direct band gaps. For larger diameters, the surface dangling bonds are not saturated, leading to surface LUMO and HOMO states that lower the gap and lead to an indirect band gap. This transition from direct to indirect gap resolves the previous disagreement in the scaling of band gap with diameter. We conclude that the electronic properties of Si NW depend sensitively on controlling the diameter and surface hydrogen coverage.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jpccck/2010/jpccck.2010.115.issue-25/jp106048u/production/images/medium/jp-2010-06048u_0002.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/jp106048u'>ACS Electronic Supporting Info</A></P>
N. Matsuda,Y. Kasugai,Y. Sakamoto,H. Nakashima,H. Matsumura,H. Iwase,N. Kinoshitay,H. Hirayama,H. Yashima,N. Mokhov,A. Levering,D. Boehnlein,K. Vazili,L. Gary,S. Wayne,K. Oishi,T. Nakamura,K. Ishibash 한국물리학회 2011 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.59 No.23
Neutron spectra in high-energy region between 1 and 100 MeV in the shield configuration of the anti-proton target station and a 120-GeV proton beam at Fermi National Accelerator Laboratory (Fermilab) were determined using the reaction rate data obtained with the multi-foil activation method. Two kinds of methods were employed for the determination of neutron spectra: one is the fitting method which is newly developed in this work, another is the unfolding method with SAND-II code. The calculations were performed using the PHITS. From the comparison between the calculated and experimental neutron spectra, it concluded that the PHITS can be used for shielding design of highenergy proton accelerators.