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자주달개비 화분모세포 미세핵 생성률의 방사선량-반응 관계
김진규,송희섭,현성희,Kim, Jin-Kyu,Song, Hi-Sup,Hyun, Soung-Hee 대한방사선방어학회 1999 방사선방어학회지 Vol.24 No.4
본 연구는 이온화 방사선이 자주달개비 화분모세포의 미세핵 생성에 미치는 영향을 분석하여 선량-반응 관계를 수립하고자 수행되었다. 자주달개비의 화분모세포는 감수분열을 통하여 4개의 꽃가루로 발전되는 생식세포로서 분열과정 중에 방사선에 노출되면 염색체 조각이 분리되어 미세핵을 형성한다. 감마선 선량이 증가함에 따라 미세핵 생성률도 증가하였으며 특히 50 cGy까지의 선량범위에서 선량별 미세핵 생성률의 최대값은 뚜렷한 선량-반응관계를 보였다. 선량-반응 관계식을 통해 볼 때 1 cGy의 부가적 선량은 100 사분자당 최대 2개씩의 미세핵을 추가로 생성시키는 것으로 나타났다. 화분모세포 미세핵 생성률의 방사선 지표성은 생물학적 방사선량 감시를 위한 필수선결사항일 뿐 아니라 공해물질의 생체 위해성 검증, 수질과 토양의 건전성 평가 등에 대한 응용성을 갖고 있다. This study was carried out to investigate the radiation dose-response of micronucleus frequencies in Tradescantia pollen mother cells. The number of micronuclei increased in the tetrads as a result of chromosome deletion after irradiation. The maximal frequency of micronuclei showed a good dose-response relationship in the range of dose $0{\sim}50$ cGy. On the basis of the relationship, a dose of 1 cGy results maximally in two additional micronuclei in 100 tetrads. The radiation dose-response relationship of micronucleus occurrence is prerequisite to biological monitoring of radiations. The micronucleus assay can be applied to biological risk assessment of environmental toxicants, and to integrity test of water or soils of interest.
권신한,오정행,송희섭,Shin-Han Kwon,Jeong-Hae Oh,Hi-Sup Song 한국작물학회 1974 Korean journal of crop science Vol.- No.15
본답에서 재배되고 있는 방사선돌연변이계통들의 도열병 저항성 검정을 위하여 절리엽편접종법이 시도되었다. 즉 포장에서 절취한 잎을 항온항습실내에서 punch압상을 만들고 그 위에 도열병균의 포자현탁액을 접종한 후 형성된 병반의 크기를 측정하여 계통간의 저항성정도를 비교한 결과 방사선조사된 풍광으로부터 Upland short row method에 의하여 선발된 저항성계통들은 모품종 풍광에 비하여 저항성으로 나타났다. 따라서 이 방법은 포장에서 재배되는 성숙식물체의 도열병저항성 검정을 위한 하나의 선발방법으로서 효과적이라 생각되었다. An attempt was made to develop a simple testing method fro blast resistance in the aged rice plant grown under the paddy field conditions. The blast resistance was tested with the detached 3rd leaves from the top of the plant, which were inoculated by dropping blast spore suspension mixed with 1% Tween 20 on the punched area and kept in 1% sucrose solution. The blast reaction was measured ten days after incubation at 26-28$^{\circ}C$ under highly humid conditions. With this method blast resistant lines were effectively identified and the cost of testing was cheap as compared with any other methods ever practiced.
Variation of several agronomic and biochemical traits in γ-ray induced mutant soybeans
심교문(Kyo Moon Shim),송희섭(Hi Sup Song),김선형(Sun Hyung Kim),이해익(Hae Ik Rhee),김남수(Nam Soo Kim) 한국육종학회 1996 한국육종학회지 Vol.28 No.3
Two soybean cultivars(Paldalkong and Bangsakong) were irradiated with gamma-ray to reduce seed size, which is an important trait for soybean sprout and the derived mutant soybeans were analyzed in several agronomic and biochemical traits. There were high levels of variations in quantitative traits among the mutants. Several mutant lines showed higher yield and smaller seed than thier parents. Qualitative traits such as seed coat color or pubescent color were also changed in a few lines. Biochemical variations were also observed among the mutants. In seed storage protein analysis, many mutant lines showed reduced intensites in β-subunits in 7S globulin than their parents and an additional band in the acidic subunit at 31KDa. Two mutant lines derived from Paldalkong showed an additional band and a shifted band of protease inhibitor by electrophoretic analysis. Variations in isozymes and RAPD were also observed among the mutants. Six isozymes(Adh, Est, Gdh, Idh, Mdh and Pgm) among eleven isozymes showed some variations and six out of ten primers showed polymorphic amplified DNA fragments among the mutnats.
김진규,김원록,이영근,김재성,송희섭,정철영,김기남,서원선,홍광표 ( Jin Kyu Kim,Won Rok Kim,Young Keun Lee,Jae Sung Kim,Hi Sup Song,Cheorl Young Jeong,Ki Nam Kim,One Sun Suh,Kwang Phyo Hong ) 한국환경생물학회 1998 환경생물 : 환경생물학회지 Vol.16 No.2
Tradescantia in BNL 4430 is a hybrid clone heterozygous for its flower color and extremely sensitive to ionizing radiation. Such a fact made people around nuclear power plants be interested in Tradescantia, a botanical tester of radiation effects. The pur pose of this study is to provide the botanical supplement to physical radiation monitor systems and also to ascertain biological safety regarding environmental radiation around nuclear power plants. Flowers collected periodically from three Tradescantia gardens (in Youngkwang NPP Jeonsigwan. City office and KAERI. respectively) were scored for pink mutation frequencies in stamen hair cells. Pink mutation frequencies in the study area were in the range of 0.82∼1.14. which were slightly lower than the annual mean background mutation frequency of 1.16. Statistical analysis of scoring data showed that there was no significant difference between three Tradescantia gardens (p>0.05). From the result, the environmental radiation level around Youngkwang nuclear power plant can be said biologically safe. Further study for verifying safety will make a role for improving public acceptance related to the peaceful use of nuclear power.