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논문 : 유세포 분석을 통한 현사시나무 3배체 선발 및 계통별 기내생장 특성
배은경 ( Eun Kyung Bae ),이효신 ( Hyo Shin Lee ),이재순 ( Jae Soon Lee ),노은운 ( Eun Woon Noh ) 한국임학회 2012 한국산림과학회지 Vol.101 No.2
세대기간이 긴 임목에 있어 3배체는 바이오매스 생산과 분자생물학적 연구 분야에서 매우 유용한 연구재료이다. 1970년대 육성된 현사시나무 4배체와 일반 2배체 간의 인공교배를 통해 3배체가 육성되었다. 유세포 분석법을 이용해 이들 F1의 배수성을 확인한 결과 14개체 중 10개체가 3배체로 선발되었다. 그 가운데 3계통의 3배체(Line-1, Line-17, Line-18)는 기내배양시 2배체에 비해 잎이 크고, 엽형이 다소 변형된 형태적 특징이 있었다. 특히 ``Line-18`` 은 줄기가 2배체에 비해 굵었으며, 뿌리 생육에 있어서 발근이 더뎠고 뿌리수도 다른 3배체나 2배체에 비해 적은 특징을 보였다. 그러나 순화율은 모두 100%에 달하였다. 본 연구결과는 3배체 현사시나무가 바이오매스 생산 및 형질전환 연구를 위한 좋은 재료가 될 수 있음을 보여주는 결과이며, 또한 유세포 분석법이 배수체 육종시 배수체를 확인하는데 효율적인 방법임을 시사한다. Triploids are a useful tool for biomass production and molecular breeding of trees with a long life span, Triploids of the poplar ``Hyunsasi`` (Populus alba × P. glandulosa) have been developed by crossing between female diploids and a male tetraploid. The tetraploid was developed around the 1970s at Korea Forest Research Institute by colchicine-induced chromosome doubling. Seedlings of the F1 generation were analyzed using flow cytometry to verify their ploidy status. The mean relative fluorescence index of 3 F1 poplars, labeled as Line-1, Line-17, Line-18, were approximately 1.5 times higher than those of diploid poplars, and the results clearly indicated that they were triploids, The phenotype of the F1 poplars included larger leaves and thicker stem than diploids, and abnormal leaf morphology, especially in the triploid ``Line-18``, Three triploid lines developed roots more slowly and had less roots than diploid. However, 3 poplar cytotypes (2x, Line-1, Line-17) rooted within 10 days on MS medium, In contrast, compared with the 3cytotypes, the Line-18 showed about 80% and 70% in the rooting rate and the number of roots, The triploid poplars could be directly utilized for biomass production and with their sterility, they could serve as basic material for genetic transformation. In addition, flow cytometric analysis proved to be an effective and reliable method for screening forest trees for their ploidy level.
문흥규,노은운,하유미,심경구,Moon, Heung-Kyu,Noh, Eun-Woon,Ha, Yoo-Mi,Shim, Kyung-Ku 한국식물생명공학회 2002 식물생명공학회지 Vol.29 No.2
히어리나무 1년생 및 10년생을 재료로 액아배양을 통한 기내번식을 시험하였다. 줄기의 증식은 zeatin과 BA의 공조처리가 효과적이었으며 MS배지에 zeatin 0.5∼3.0 mg/L, 0.2 mg/L BA 처리시 주효하였다. 1년생이 10년생보다 전반적으로 증식 및 생장이 양호하였으며, 배양 6개월 후에는 10년생에서도 매월 절편 당 3개의 줄기유도가 가능하였다. 기내 줄기의 발근은 1년생은 97%, 10년생은 62%를 나타내었고, 토양이식시 1 년생 유래 배양묘는 67%, 10년생은 48%생존되어 모수령에 따른 차이를 나타냈다. 본 실험결과 히어리나무의 액아배양으로 유시, 성숙목의 대량번식이 가능함을 보여주었으나 선발개체의 효율적인 기내번식을 위해서는 재유령화의 기술개발과 토양순화율을 증진시켜야 하는 것으로 나타났다. We have developed an in vitro micropropagation system via shoot formation from axillary buds using nodal segments of Corylopsis coreana. Explants from both juvenile tree (one-year-old greenhouse stock seedlings) and mature tree (ten-years-old tree in nursery) were compared with regard to propagation efficiency. Combined treatment of both BA and zeatin were effective on shoot proliferation since the best result was obtained on MS medium supplemented with 0.5∼3.0 mg/L zeatin and 0.2 mg/L BA. Generally, juvenile explants were better in both shoot proliferation and growth than mature explants. However, as the duration of in vitro culture was proceed to 6 months, explants from mature tree also produced three shoots per explant. Distinctive differences in rooting and adaptability to soil of shoots obtained from mother trees. Whereas shoots originated from juvenile explants rooted as high as 97%, those from adult explants showed 62% rooting. Similar result was also observed in soil acclimatization. The plantlets derived from juvenile plants survived 67%, while only 48% of those from adult trees survived. The results showed a possibility of the micropropagation of Corylopsis coreana through shoot formation from axillary buds. In addition, the advance of the research still remain to enhance the frequency of acclimatization of plantlets from mature trees for practical application.
포플러류의 식물체와 미토콘드리아의 동위효소 분석에 의한 몇 동위효소의 세포소기관적 (細胞小器管的) 위치
류장발,노은운,손두식 ( Jang Bal Ryu,Eun Woon Noh,Doo Sik Son ) 한국산림과학회 1991 한국산림과학회지 Vol.80 No.1
Subcellular location of five isozymes in Populus species was studied by isozyme analysis with plant and mitochondria. Isozymes analyzed were ADH, 6-PGD, MDH, PGI, and Diaptrorase. ADH seems to be cytosolic isozyme encoded with nuclear genes at one locus, while 6-PGD seems to be mitochondrial one. MDH showed three hands in cytosol and four other bands in mitochondria. Three cytosolic hands were showed for PGI. Diaphorase showed one mitochodrial band and two cytosofic bands which seemed to the encoded by nuclear genes at one locus.
P. C. R 기법을 이용한 들메나무 DNA sequence 의 변이조사
나천수(Chun Soo Na),노은운(Eun Woon Noh),김영중(Young Joong Kim),신창호(Chang Ho Shin),송원섭(Won Sup Song),김세현(Sea Hyun Kim) 한국산림과학회 1992 한국산림과학회지 Vol.81 No.4
It has been reported that there are two distinct phenotypes in Frarinus mandshurica Rupr. growing in Korea. Recently developed polymerase chain reactioniPCR) was used to detect DNA sequence polymorphism in the species. Using a thermostable DNA polymerase and synthetic DNA primers, unknown DNA sequences from the species were randomly amplified. The two types of the species produced different DNA amplification pattern with three different primers tested, Although DNA polymorphism was detected among individuals within types, each type has its own distinct pattern. The two types could be easily differentiated by trier characteristic predominant bands.