몇 가지 식물추출물이 배양 NIH3T3 섬유모세포의 세포생존율과 세포부착률에 미치는 세포독성에 관한 연구

임요섭 ( Yo-sup Rim ),송원섭 ( Won-seob Song ),서영미 ( Young-mi Seo ),박승택 ( Seung-taeck Park ),김신무 ( Shin-moo Kim ) 대한임상검사과학회 2010 대한임상검사과학회지(KJCLS) Vol.42 No.3

This study was aimed to clerify the cytotoxicity of some plant extracts such as Hosta longissima HONDA (HL), Hemerocallis fulva var. Kwanso REGL (HFVK), Hemerocallis fulva L (HF), Macrocapium officinale NAKAI (MO) and Mentha canadensis var. piperascens HARA (MCVP), the cultured NIH3T3 fibroblasts were treated with 25, 50, 100, 150 and 200 μg/mL of five kinds of plant extracts for 48 hours, respectively. The cytotoxicity of plant extracts was measured by MTT and NR assays for the cell viability, and XTT assay for the cell adhesion activity. In this study, HL, MO and FHVK extracts showed the range of midtoxic-non toxic by the criteria of chemical cytotoxicity. While, the HF and MCVP extracts showed midtoxic. In the extract cytotoxicity, HL, MO and FHVK extracts showed non-toxic by the criteria of extract cytotoxicity. While, HF extract was determined as lower-toxic. In the responsive sensitivity of each plant extract on colorimetric assays, HF extract was sensitive to mitochondrial enzyme by MTT assay, lysosomal enzyme by NR assay and mitochondrial nucleus by XTT assay. While, MCVP extract was sensitive to mitochondrial enzyme by MTT assay and lysosomal enzyme by NR assay than other assays. While, HL, HFVK and MO extracts were most sensitive to NR assay. Cell culture is one of useful materials in the screening of cytotoxic and recovary effect on the putative chemical agents or plant extract. And also, colorimetric assay is regarded as very useful tools for quantitative measurement of cytotoxic effect on plant extracts in vitro.

낙동강 부영양화와 수질환경요인의 통계적 분석

김미숙,정영륜,서의훈,송원섭,Kim, Mi-Suk,Chung, Young-Ryun,Suh, Euy-Hoon,Song, Won-Sup 한국조류학회(藻類) 2002 ALGAE Vol.17 No.2

Influences of vrious environmental factors on the eutrophication of Nakdong River were analyzed statistically using water samples collected from 1 January, 1999, to 30 September, 2001 at Namji area. The relationships between the concentration of chlorophyll α (eutrophication index) and environmental factors and were analyzed to develop a statistical model which can predict the status of eutrophication. The concentation of chlorophyll α ranged from 66.2 mg · $m^{-3}$ to 70.8 mg · $m^{-3}$ during dry winter season and the average concentration during this study period was 35.5 mg · $m^{-3}$ Namji area of Nakdong River was in the hypereutrohic stage in terms of water quality. Stephanodiscus sp. and Aulacoseria granulata var. angustissima were dominant species during the witnter to spring time and summer to autumn period, respectively. Based on the correlation analysis and the analysis of variance between chlorophyll α concentration and environmental factors, significantly high positive relationships were found in the order of BOD> pH> COD > KMnO₄ consumption > DO > conductivity > alkalinity. In contrast to these factors, significantly negrative relationships were found as in the order of $PO₄^{3-}-P$ >water level>the rate of Namgang-dam discharge > NH₃-N> the rate of Andong-dam discharge> the rate of Hapchoen-dam discharge. Based on the factors analysis of environmental factors on the concentration of chlorophyll α, we obtained five factors as follows. The first factor included water level, pH, turbiditiy, conductivity, alkalinity and the rate of Namgang-dam discharge. The second factor included water temperature DO, NH₄+-N, NO₃- -N. The third factor included KMnO₄ consumption COD and BOD. The fourth factor included the rate of Andong-dam discharge, the rate of Hapcheon-dam discharge, and the rate of Imha-dam discharge. The final factor included T-N T-P and $PO₄^{3-}-P$ > concentration. We derived two statistica models that can predict the occurrence of eutrophication based on the factors by factor analysis, using regression analysis. The first model is the stepwise regression model whose independent variables are the factors produced by factor analysis : chl α (mg · $m^{-3}$ = 42.923+(18.637 factor 3) + (-17.147 factor 1) + (-12.095 factor 5) + (-4.828 factor 4). The second model is the alternative stepwise regression model whose independent variables are the sums of the standardized main component variables:chl α (mg · $m^{-3}$ = 37.295+(7.326 Zfactor 3) + (-2.704 Zfactor 1)+(-2.341 Zfactor 5).

Cholescintigrphy 의 적응증 및 그 의의

채권묵(Kwon Mook Chae),송원섭(Won Sup song),김창근(Chang Guhn Kim) 대한소화기학회 1987 대한소화기학회지 Vol.19 No.1

N/A Recently, when the hepatobiliary disease was suspected, the ultrasonography was performed usually for screening test in most hospital. Since the introduction of technetium -99m- labeled cholescintigraphic agent in the mid-1970s, there has been extensive investigation of their diagnostic value and significance as screening test of biliary tract disorders. The diagnostic value of cholescintigraphy was accepted in diagnosis of acute cholecystitis, but the other diagnostic value of cholescintigraphy is remained controversy in diagnosis of other hepatobiliary diseases. Therefore, we conducted a retrospective study of 152 patients that had been performed cholescintigraphy at Wonkwang University from 1984. The result of cholescintigraphic findings were classified into 7 criterias as follow, without information of patients history, physical findings and laboratory data, criteria 1; normal cholescintigraphy (all visualization within 1 hour) criteria 2; acute cholecystitis (non-visualization of gall-bladder within 2 hours, prompt hepatic uptake, good CBD and bowel excretion), criteria 3; chronic cholecystitis (prompt hepatic uptake, good bowel and CBD excretion, delayed visualization of gall-bladder within 2 hours), criteria 4; complete CBD obstruction (non-visualization except liver), criteria 5; partial CBD obstruction (delayed biliary tree excretion), criteria 6; poor hepatic uptake, criteria 7; functional CBD obstruction (gall bladder, CBD visualization within 1 hour but no bowel activity). We classified the patient into 7 criterias of cholescintigraphic findings and compared the cholescintigraphic findings to the confirmed diagnosis. The results are as fotlows; 1) The cholescintigraphy was valuable study to rule out the acute cholecystitis in the patient who was suspected as other disease. 2) When the acute cholecystitis was suspected primarily, cholescintigraphy was valuable method, but on comparing the diagnostic value of ultrasonography, there was no significant difference. 3) The 5 cases among the 10 cases that were classified as chronic cholscystitis(criteria 3) were false positve. The cholescintigraphy was not valuabe method for diagnosis of chronic cholecystitis. 4) When the findings of cholescintigraphy showed partial or complete biliary obsruction, 16 cases of 17 cases were true positve. Therefore, these cholescitigraphic findings seemed to be valuable in diagnosis of biliary obsruction, but the 16 cases among the total 35 cases of bilary obstruction in all criterias were detected in cholescintigraphy. So, the sensitivity of this study was 45.7%.

RAPD 分析에 의한 無窮花 品種間의 類緣關係 및 遺傳的 變異

Seon Ha Lee(李善河),Chi Hyun Kim(金致賢),Won Sup Song(宋源燮),Ill Sup Nou(盧一燮) 한국육종학회 1996 한국육종학회지 Vol.28 No.4

Genetic variation and phylogenetic relationship among 84 varieties of Hibiscus syriacus were investigated by RAPD analysis. When total proteins of 16 varieties were analyzed by SDS-PAGE, many protein bands were detected, but no differences among the varieties were observed. The optimum condition for PCR reaction was 150 mM dNTP, 0.2 unit Tag polymerase, and 1.5 or 2.0 mM MgCl₂ for 10㎕ reaction volume. Amplication with twenty two random primers resulted in polymorphism among the varieties and therefore enabled us to study genetic variation study. A total of 121 bands were observed by the RAPD analysis of the 84 varieties, 2 to 8 bands for each primer. The number of average bands were 5.5 for all the primers used, and 5.81 and 4.67 for 16 OPB primers and for 6 TAA primers, respectively. No differences between 10-mer and 12-mer primers were observed in detected band numbers. Among the 84 varieties, the most closely related two varieties appeared to be Serenade and Shihai (similarity value=0.91), and the least closely related were Chungmu and Kijibato (0.39). The 84 varieties could be classified into 6 groups (group A-F) based on RAPD analysis. Generally, domestic varieties did not-fit into a single specific group, but belonged to groups A, B, D and E along with introduced foreign varieties, indicating most of the domestic varieties were developed by crossing with introduced foreign varieties. When 12 varieties with similar genetic backgrounds were further analyzed, a total of 99 bands were detected and the varieties could be classified into 3 groups at the similarity value of 0.5. Among Tanshim (2n), Paektanshim (2n) and Kaeryang-tanshim (4n), Tanshim and Kaeryang-tanshim were more closely related than Tanshim and Paektanshim. Shihai, a variety from Japan, showed closer relationship with Parangsae than with Chabae. These reasons were widely discussed in this paper.

P. C. R 기법을 이용한 들메나무 DNA sequence 의 변이조사

나천수(Chun Soo Na),노은운(Eun Woon Noh),김영중(Young Joong Kim),신창호(Chang Ho Shin),송원섭(Won Sup Song),김세현(Sea Hyun Kim) 한국산림과학회 1992 한국산림과학회지 Vol.81 No.4

It has been reported that there are two distinct phenotypes in Frarinus mandshurica Rupr. growing in Korea. Recently developed polymerase chain reactioniPCR) was used to detect DNA sequence polymorphism in the species. Using a thermostable DNA polymerase and synthetic DNA primers, unknown DNA sequences from the species were randomly amplified. The two types of the species produced different DNA amplification pattern with three different primers tested, Although DNA polymorphism was detected among individuals within types, each type has its own distinct pattern. The two types could be easily differentiated by trier characteristic predominant bands.