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1인 가구를 위한 공동구매 및 정보 공유 커뮤니티 서비스
이예은(Yeeun Lee),김규리(Gyuree Kim),이해서(Haeseo Lee),정은혜(Eun-Hye Jeong),안형태(Hyeongtae Ahn),오병우(Byoung-Woo Oh) 한국정보기술학회 2022 Proceedings of KIIT Conference Vol.2022 No.6
국내 1인 가구의 비중이 커지면서 이와 관련된 맞춤 서비스의 수요 또한 증가하고 있다. 이러한 사회적 흐름에 주목하여 1인 가구 관련 서비스가 개발되고 있다. 그러나 사용자의 요구를 종합하여 다양한 기능을 제공하는 시스템을 찾기는 어렵다. 현 상황을 고려하여 본 논문에서는 1인 가구를 위한 맞춤형 커뮤니티 서비스를 제안한다. 본 논문에서 제안하는 서비스는 사용자의 경제적 부담을 줄이기 위해 배달 및 배송 공동구매와 OTT 플랫폼 공유 구독자 모집을 지원한다. 그리고 1인 가구 정책 및 유용한 생활 정보를 공유할 수 있다. As the proportion of single-person households in Korea increases, the demand for customized services related to this is also increasing. Paying attention to these social trends, services related to single-person households are being developed. However, it is difficult to find a system that provides various functions by synthesizing user needs. Considering the current situation, this paper proposes a customized community service for single-person households. The service proposed in this paper supports the group purchase of delivery and delivery and the recruitment of OTT platform sharing subscribers to reduce the economic burden of users. You can also share single-person household policies and useful life information.
DNA binding fluorescent proteins for the direct visualization of large DNA molecules
Lee, Seonghyun,Oh, Yeeun,Lee, Jungyoon,Choe, Sojeong,Lim, Sangyong,Lee, Hyun Soo,Jo, Kyubong,Schwartz, David C. Oxford University Press 2016 Nucleic acids research Vol.44 No.1
<P>Fluorescent proteins that also bind DNA molecules are useful reagents for a broad range of biological applications because they can be optically localized and tracked within cells, or provide versatile labels for <I>in vitro</I> experiments. We report a novel design for a fluorescent, DNA-binding protein (FP-DBP) that completely ‘paints’ entire DNA molecules, whereby sequence-independent DNA binding is accomplished by linking a fluorescent protein to two small peptides (KWKWKKA) using lysine for binding to the DNA phosphates, and tryptophan for intercalating between DNA bases. Importantly, this ubiquitous binding motif enables fluorescent proteins (<I>K</I><SUB>d</SUB> = 14.7 μM) to confluently stain DNA molecules and such binding is reversible via pH shifts. These proteins offer useful robust advantages for single DNA molecule studies: lack of fluorophore mediated photocleavage and staining that does not perturb polymer contour lengths. Accordingly, we demonstrate confluent staining of naked DNA molecules presented within microfluidic devices, or localized within live bacterial cells.</P>
MALDI-TOF Analysis of Binding between DNA and Peptides Containing Lysine and Tryptophan
( Seonghyun Lee ),( Sojeong Choe ),( Yeeun Oh ),( Kyubong Jo ) 한국질량분석학회 2015 Mass spectrometry letters Vol.6 No.3
Here, we demonstrate the use of MALDI-TOF as a fast and simple analytical approach to evaluate the DNA-binding capability of various peptides. Specifically, by varying the amino acid sequence of the peptides consisting of lysine (K) and tryptophan (W), we identified peptides with strong DNA-binding capabilities using MALDI-TOF. Mass spectrometric analysis reveals an interesting novel finding that lysine residues show sequence selective preference, which used to be considered as mediator of electrostatic interactions with DNA phosphate backbones. Moreover, tryptophan residues show higher affinity to DNA than lysine residues. Since there are numerous possible combinations to make peptide oligomers, it is valuable to introduce a simple and reliable analytical approach in order to quickly identify DNA-binding peptides.
간호학생의 객관구조화 임상시험(OSCE) 후 피드백 유형에 따른 임상수행 자신감과 피드백 만족도 효과 비교
길은하(Gil, Eunha),오희영(Oh, Heeyoung),신선경(Shin, Seonkyoung),박연희(Park, Yeonhee),이예은(Lee, Yeeun),박정아(Park, Jeong Ah) 기본간호학회 2015 기본간호학회지 Vol.22 No.3
Purpose: The purposes of this study were to compare confidence in practice of intermittent gavage tube feeding and participants’ satisfaction by three types of feedback; professor verbal feedback, professor feedback with smartphone video, and peer feedback with smartphone video. In addition, frequently failed items in the intermittent gavage tube feeding procedure were analyzed. Methods: Data were collected from a convenience sample of 78 nursing college students in November 2014. Students were randomly assigned to the control group, experimental group I (smartphone video with professor feedback) or group II (smartphone video with peer feedback). Data were analyzed using descriptive statistics including chi-square test, ANOVA, and Scheffe test with SPSS 21.0. Results: Confidence in practice of intermittent gavage tube feeding and satisfaction with feedback were highest in experimental group I that had professor feedback with smartphone video. For the procedure, the most frequently failed item was giving an explanation to patients about the purpose and the procedure of tube feeding. Conclusion: The results indicate that professor verbal feedback with smartphone video is the most benefit to the nursing students in acquiring core nursing practice skills.
MALDI-TOF Analysis of Binding between DNA and Peptides Containing Lysine and Tryptophan
Lee, Seonghyun,Choe, Sojeong,Oh, Yeeun,Jo, Kyubong Korean Society for Mass Spectrometry 2015 Mass spectrometry letters Vol.6 No.3
Here, we demonstrate the use of MALDI-TOF as a fast and simple analytical approach to evaluate the DNA-binding capability of various peptides. Specifically, by varying the amino acid sequence of the peptides consisting of lysine (K) and tryptophan (W), we identified peptides with strong DNA-binding capabilities using MALDI-TOF. Mass spectrometric analysis reveals an interesting novel finding that lysine residues show sequence selective preference, which used to be considered as mediator of electrostatic interactions with DNA phosphate backbones. Moreover, tryptophan residues show higher affinity to DNA than lysine residues. Since there are numerous possible combinations to make peptide oligomers, it is valuable to introduce a simple and reliable analytical approach in order to quickly identify DNA-binding peptides.
Kang, Yujin,Lee, Jinyong,Kim, Jisoo,Oh, Yeeun,Kim, Dogeun,Lee, Jungyun,Lim, Sangyong,Jo, Kyubong The Royal Society of Chemistry 2016 The Analyst Vol.141 No.14
<P>Consumption of alcohol injures DNA, and such damage is considered to be a primary cause for the development of cancer and many other diseases essentially due to reactive oxygen species generated from alcohol. To sensitively detect alcohol-induced DNA lesions in a biological system, we introduced a novel analytical platform for visualization of single genomic DNA molecules using E. coli. By fluorescently labelling the DNA lesions, our approach demonstrated, with the highest sensitivity, that we could count the number of DNA lesions induced by alcohol metabolism in a single bacterial cell. Moreover, our results showed a linear relationship between ethanol concentration and the number of DNA lesions: 0.88 lesions per 1% ethanol. Using this approach, we quantitatively analysed the DNA damage induced by exposure to alcoholic beverages such as beer (5% ethanol), rice wine (13%), soju (20%), and whisky (40%).</P>