흰쥐에서 ${\alpha}$-Adrenaline 效能 藥物등이 피마자油 誘發泄瀉에 미치는 영향

허인회,이도익,이종흔,김동휘,Huh, In-Hoi,Lee, Do-Ik,Lee, Jong-Heun,Kim, Dong-Whee 대한약학회 1984 약학회지 Vol.28 No.4

The effect of lofexidine, clonidine, and other drugs on castor oilinduced diarrhea in rats was investigated. Castor oil was administered orally and drugs were administered intraperitoneally. The results were as follows. 1. Lofexidine(0.1-43{\mu}mol$/kg) blocked dose dependently caster oil-induced diarrhea like clonidine, but its potency was weaker than that of clonidine, 1. Yohimbine (1-10{\mu}mol/kg$) antagonized the antidiarrheal action of lofexidine and clonidine. 3. Prazosin (3-30{\mu}mol/kg$) did not antagonize the antidiarrheal action of lofexidine and clonidine. 4. Metoclopramide, bromocriptine mesylate, and nadolol did not affect antidiarrheal action of lofexidine significantly. 5. Loperamid prolonged the antidiarrheal action of lofexidine.

Rat에서 Cromakalim에 의해 유발된 혈관이완 및 혈압강하작용에 대한 Glipizide의 억제작용

허인회(In Hoi Huh),안형수(Hyung Soo Ann),윤성훈(Seong Hun Yoon) 大韓藥學會 1991 약학회지 Vol.35 No.3

The inhibitory effects of glipizide on cromakalim-induced relaxation of aortae and hypotension in the anesthetized rats was examined. In rat thoracic aortic rings pre-contracted with norepinephrine, cromakalim produced a relaxation sustainedly. This relaxation was completely inhibited by pre- or post-treatment of glipizide. In the anesthetized rat, cromakalim produced a rapid and sustained fall in the arterial blood pressure. This hypotensive action of cromakalim was abolished by pre- or post-treatment of glipizide. It is suggested that glipizide is the potent inhibitor of cromakalim, K+ channel opener, in the rats.

Dopamine이 토끼 유두근의 수축력과 활동전압에 미치는 영향

허인회(In Hoi Huh),박종완(Jong Wan Park) 대한약학회 1988 약학회지 Vol.32 No.6

In order to clarify the receptor types and mechanisms underlying the positive inotropic effect of dopamine on the mammalian ventricular myocardium, the action potential, its first derivatives and isometric contraction of the rabbit papillary muscle were recorded using a force transducer and glass capillary microclectrodes filled with 3M KCl. The results were as follows; (1) In normal Tyrode solution, the contractile force was increased and duration of action potential was shortened with increments of dopamine concentration (10-6-10-4M). (2) The dose-response curve was markedly shifted to the right by pretreatment with reserpine (5mg/kg i.p., 24 hrs prior to the experiment). (3) In 19mM K+-Tyrode solution, the duration of action potential, maximum rate of rise (Vmax) of action potential and overshoot were significantly increased with increments of dopamine concentration (10-6-10-4M). (4) The inotropic effect of dopamine on the rabbit papillary muscle pretreated with reserpine was antagonized by atenolol (10-6M), but not by phentolamine (3 X 10-6M). (5) In rabbit papillary muscle partially depolarized by 19mM K+-Tyrode solution, slow electrical response (calcium mediated action potential) as well as contraction were restored by dopamine (10-4M); this restoration was blocked by calcium antagonists (3 X 10-5M LaCl3.6H20, 3 X 10-6M diltiazem) or beta-adrenoceptor antagonist (3 X 10-6M atenolol), but not affected by alpha-adrenoceptor antagonist (10-5M phentolamine, 3 X 10-6M yohimbine) or vascular dopaminergic receptor antagonist (10-5M haloperidol). The above results may be interpreted as that the positive inotropic effect of dopamine through both direct and indirect action are caused by increase in slow inward current (Ca2+ influx into themyocardial cell), and the direct action is mainly due to the stimulation of beta-adrenoceptors in the rabbit papillary muscle.

l-Muscone 의 심혈관계에 관한 약리연구

조태순(Tai Soon Cho),김낙두(Nak Doo Kim),허인회(In Hoi Huh),권광일(Kwang Il Kwon),박석기(Seok Ki Park),심상호(Sang Ho Shim),신대희(Dae Hee Shin),박대규(Dai Kyu Park) 한국응용약물학회 1997 Biomolecules & Therapeutics(구 응용약물학회지) Vol.5 No.3

In order to investigate the pharmacological properties of l-muscone, effects of l-muscone and musk were studied on the cardiovascular system with various experimental models. In isolated rat aorta, l-muscone and musk made the relaxation of blood vessels in maximum contractile response to phenylephrine (10^(-6) M) in endothelium-containing rings of the rat aorta, but not in endothelium-denuded rings. However, l-muscone and musk in the presence of the inhibitor of NO synthase and guanylate cyclase did not make the relaxation of blood vessels. In spontaneously hypertensive rats (SHRs), l-muscone and musk slightly reduced blood pressure but significantly decreased heart rate. In the isolated perfused rat hearts, l-muscone and musk did not affect significantly on LVDP, contractile force, coronary flow and (-dp/dt)/(+dp/dt). These results suggest that l-muscone and musk have weak cardiovascular effects with relaxation of blood vessel and decrease of heart rate, but without significant cardiac functions.

Etomidoline과 Nefopam. HCl 병용투여시의 진경 및 진통 효과에 관한 연구

허인회(In Hoi Huh),안형수(Hyung Soo Ann) 대한약학회 1981 약학회지 Vol.25 No.1

Etomidoline is a new synthetic atropine-like drug. The present investigation aimed to study the combined effects of etomidoline and neofopam hydrochloride which has an analgesic and muscle relaxant activity, compared with the effects of each drugs. Acute toxicities (ID50) in mouse were 132mg/Kg (p.o.)and 49mg/Kg (i.p.) when combination ratio of etomidoline and neofopam was 1:5 and 103mg/Kg (p.o.) and 30mg/Kg (i.p.) with the ratio of 1:10. Etomidoline showed more potent anticholinergic effects than neofopam in the isolated rat intestine. Whereas, antibarium effects were twice as active with neofopam than with etomidoline. When etomidoline and neofopam were added in combination ratio of 1:5, papaverine-like activity was increased, but no changes of anticholinergic effect were observed. Analgesic effect was measured by the anti-writhing method of Whittle in mice. Both of the concurrent and single administration of etomidoline and nefopam reduced significantly the writhing number and the effect of the concurrent administration was more active than that of single, and 1:5 combination was slightly more potent than 1:10. Each drug or the combined drug was administered to mice and observed the change of the pupil size. Pupil sizes were increased with each drug and with combined drug, although there were no significant differences between the each group of drugs. However, those effects were less than that of atropine sulfate.

흰쥐의 뮤시몰투여에 의한 배뇨반사억제효과에 대한 벤조디아제핀 수용체의 영향

허인회(In Hoi Huh),오호정(Ho Jung Oh) 大韓藥學會 1992 약학회지 Vol.36 No.5

The correlation between GABA receptors(GABAA and GABAB receptor) and benzodiazepine receptor on the saline infusion-induced micturition reflex contraction was studied in the female rat. To investigate the effect of gammar-aminobutyric acid(GABA) on the micturition reflex, exogenous GABA(10mg/kg) and GABA transaminase inhibitor(aminooxyacetic acid; AOAA 1mcg) were administered intravenously(i.v.) and intracerebroventriculary(i.c.v.), respectively. In result, both GABA and AOAA inhibited the saline induced micturition reflex contraction. This AOAA induced inhibition of micturition reflex was blocked by both bicuculine, GABAA receptor antagonist, and Ro 15-1788, benzodiazepine receptor antagonist. Muscimol, GABAA receptor antagonist(1mcg i.c.v., 3mcg intrathecal; i.t., 1mg/kg i.v.) and baclofen, GABAB receptor agonist(0.1mcgi.c.v., 3mcg i.t., 1mg/kg i.v.) also inhibited the bladder contraction. Pretreatment of bicuculline(1mcg i.c.v.), but not of 5-aminovaleric acid(AVA, 1mcg i.c.v.), GABAB receptor antagonist blocked the central inhibition of muscimol. These inhibitory effects were reversed by Rol5-1788 but were potentiated by flurazepam, benzodiazepine receptor antagonist. On the other hand, the inhibitory effects of baclofen were not affected by Ro 15-1788. Diazepam and flurazepam also inhibited the micturition reflex contraction when they were administered 3mcg i.c.v., 10mcg i.t., 10mcM, 30mcM transurethrally, respectively. In conclusion, these results suggest that the micturition reflex is mediated by GABAA, GABAB receptor and benzodiazepine receptor. The bezodiazepines increase the receptor binding of GABA to the GABAA receptor, so that the benzodiiazepines show the synergistic effect on the inhibition of the micturition reflex contraction, but not to the GABAB receptor.

신우황청심원액의 심혈관계에 관한 약효연구

이선미(Sun Mee Lee),조태순(Tai Soon Cho),안형수(Hyung Soo Ann),허인회(In Hoi Huh),심상호(Sang Ho Shim),박석기(Seok Ki Park),신대희(Dae Hee Shin),박대규(Dai Kyu Park),김낙두(Nak Doo Kim),권광일(Kwang Il Kwon) 한국응용약물학회 1997 Biomolecules & Therapeutics(구 응용약물학회지) Vol.5 No.4

In order to investigate the pharmacological properties of New Woohwangchungsimwon Liquid (NCL), effects of Woohwangchungsimwon Liquid (CL) and NCL were compared. In isolated rat aorta, NCL and CL showed the relaxation of blood vessels in maximum contractile response to phenylephrine (10^(-6) M) without regard to intact endothelium or denuded rings of the rat aorta. Furthermore, the presences of the inhibitor of NO synthase and guanylate cyclase did not affect the relaxation of NCL and CL. NCL and CL inhibited the vascular contractions induced by acetylcholine, prostaglandin endoperoxide or peroxide in a dose-dependent manner. In conscious spontaneously hypertensive rats (SHRs), NCL and CL significantly decreased heart rate. NCL and CL, at high doses, had a negative inotropic effect that was a decrease of LVDP and (-dp/ dt)/(+dp/dt) in the isolated perfused rat hearts, and also decreased the contractile force and heart rate in the isolated rat right atria. In excised guinea-pig papillary muscle, NCL and CL had no effects on parameters of action potential at low doses, whereas inhibited the cardiac contractility at high doses. These results suggested that NCL and CL have weak cardiovascular effects with relaxation of blood vessels and decrease of heart rate, and that this effect is no significant differences between two preparations.

쥐에서 비스테로이드성 항염증제의 투여후 항염효과와 혈청 및 조직내 구리함량변화에 관한 연구

허인회(In Hoi Huh),임철빈(Churl Bin Yim) 대한약학회 1982 약학회지 Vol.26 No.2

This paper presents anti-inflammatory effects of nonsteroidal anti-inflammatory drugs and their copper complexes, and the change of content of copper in serum, liver, brain and edema foot induced by 1% carrageenan in rats, and also investigation of stomach hemorrhage. The results were as follows. 1. The content of copper decreased in liver and brain, however, the concentration of copper significantly increased in serum and edema site after carrageenan injection in rats. 2. The content of copper in serum and edema site was decreased after administration of anti-inflaminatory drugs. 3. Edema inhibition rate of aspirin was, higher than that of copper (II) aspirinate, but edema inhibition rate of copper complex of naproxen was markedly higher than that of naproxen. 4. Hemorrhage of stomach of copper salicylate was higher than that of sodium salicylate, but hemorrhage of stomach of sodium naproxen was higher than that of copper naproxen.

신우황청심원의 심혈관계에 대한 약효

조태순(Tai Soon Cho),이선미(Sun Mee Lee),허인회(In Hoi Huh),안형수(Hyung Soo Ann),권광일(Kwang Il Kwon),박석기(Seok Ki Park),심상호(Sang Ho Shim),신대희(Dae Hee Shin),박대규(Dai Kyu Park),김낙두(Nak Doo Kim) 대한약학회 1997 약학회지 Vol.41 No.6

In order to investigate the pharmacological properties of New Woohwangehungsimwon Pill (NWCH). Effects of Woohwangehungsimwon Pill (WCH) and NWCH were compared using various experimental models. In isolated rat aorta, NWCH and WCH showed the relaxation of blood vessels in maximum contractile response to phenylephrine (10-6M) without regard to endothelium containing or denuded rings of the rat aorta. Furthermore, the presence of the inhibitors of NO synthase and guanylate cyclase did not affect significantly the relaxative effects of NWCH and WCH. NWCH and WCH inhibited the vascular contractions induced by acethylcholine, prostaglandin endoperoxide or peroxide in a dose-dependent manner. In conscious spontaneously hypertensive rats(SHRs), NWCH and WCH decreased significantly heart rate. These, at high doses, had a negative inotropic effect that was a decrease of LVDP and (-dp/dt)/(+dp/dt) in the isolated perfused rat hearts, and also decreased the contractile force and heart rate in the isolated rat right atria. In excised guinea-pig papillary muscle, these had no effects on parameters of action potential at low doses, whereas inhibited the cardiac, contractility at high doses. Furthermore, these had a significant inhibitory effects on heart acceleration in normotensive rats and SHRs. These results suggested that NWCH and WCH have weak cardiovascular effects, and that there is no significant differences between two preparations.

신원방우황청심원액의 심혈관계에 관한 약효

조태순(Tai Soon Cho),이선미(Sun Mee Lee),김낙두(Nak Doo Kim),허인회(In Hoi Huh),안형수(Hyung Soo Ann),권광일(Kwang Il Kwon),박석기(Seok Ki Park),심상호(Sang Ho Shim),신대희(Dae Hee Shin),박대규(Dai Kyu Park) 한국응용약물학회 1999 Biomolecules & Therapeutics(구 응용약물학회지) Vol.7 No.1

In order to investigate the pharmacological properties of New Wonbang Woohwangchungsimwon Liquid (NSCL), effects of Wonbang Woohwangchungsimwon Liquid (SCL) and NSCL were compared. In isolated rat aorta, NSCL and SCL showed the relaxation of blood vessels in maximum contractile response to phenylephrine (10^(-6) M) regardless to intact endothelium or denuded rings of the rat aorta. Furthermore, the presences of the inhibitor of NO synthase and guanylate cyclase did not affect the relaxing effect of NSCL and SCL. NSCL and SCL inhibited the vascular contractions induced by acetylcholine, prostaglandin endoperoxide or peroxide in a dose-dependent manner. In conscious spontaneously hypertensive rats (SHRs), NSCL and SCL significantly decreased heart rate. NSCL and SCL, at high doses, had a negative inotropic effect that was a decrease of left ventricular developed pressure and (-dp/dt)/(+dp/dt) in the isolated perfused rat hearts, and also decreased the contractile force and heart rate in the isolated rat right atria. In excised guinea-pig papillary muscle, NSCL and SCL had no effects on parameters of action potential such as resting membrane potential, action potential amplitude, APD_(90) and V_(max) at low doses, whereas inhibited the cardiac contractility at high doses. These results suggested that NSCL and SCL have weak cardiovascular effects with relaxation of blood vessels and decrease of heart rate, and that this effect is no significant differences between cardiovascular effects of two preparations.