옻나무 목부로부터 분리한 Favonoids가 Paraquat 독성의 경감기전 검색

김정수,조희숙,강혜옥,한갑이,정민화,최종원 한국생명과학회 2003 생명과학회지 Vol.13 No.6

옻나무(Rhus verniciflua Stokes)로부터 제초제인 paraquat(PQ)의 독성 경감기전을 추구할 목적으로 ethylacetate 분획에서 분리한 fustin 및 sulfuretin을 실험동물에 투여하고서 혈액학적 변화 및 간장 중 활성산소에 미치는 영향 검토한 결과 혈액 생화학적 인자 분석을 종합해 보면 옻나무의 메탄을 엑스(250 mg/kg), 에틸아세테이트 엑스(250 mg/kg), fustin(10 mg/kg) 및 sulfuretin(10 mg/kg)을 2주간 각각 경구투여 하고서 PQ를 투여하므로서 PQ의 독성이 유의성 있게 억제되었다. in vivo에서 옻나무의 분획 및 성분의 투여는 혈액생화학적 검사에서 PQ에 의해 유도된 간독성 지표인 s-GPT, s-GOT치, 신장독성지표인 BUN, creatinine치 및 조직손상의 지표인 ALP, MDA치를 억제하였다 폐조직중의 MDA함량, ALP활성 및 collagen함량도 억제하였다. 이로써 옻나무에는 PQ의 폐독성 및 각 장기의 독성을 효과적으로 경감시켜 줄 수 있는 물질이 함유되어 있는 것으로 사료된다. In this study, we investigated to elevate the modulatory effect of flavonoid(fustin, sulfuretin, 10 mg/kg) which was isolated from Rhus verniciflua Stokes(RVS) in male Sprague-Dawley rats for 2 weeks on the toxicity of paraquat. In the flavonoids pretreated groups, glutamic oxaloacetic transaminase, glutamic pyruvic transaminase, blood urea nitrogen, creatinine, malondialdehyde and alkaline phosphatase activity in serum and malondialdehyde, alkaline phosphatase activity and collagen in lung tissue which was induced paraquat toxicity were slightly decrease compared to the normal group. In the lung tissue of flavonoids pretreated groups, malodialdehyde value, G-6-phosphatase activity and collagen synthesis were recovered to tile normal values and alkaline phosphatase activity was increased. From these results, we concluded that flavonoids which were isolated from RVS is an effective agent to inhibit the pulmonary and internal toxicities and hence we concluded that acitive components of fustin and sulfuretin which were isolated from RVS might be removed free radicals induced by paraquat.

간섬유화 동물에서 옻나무 목부로부터 분리한 flavonoids의 독성 경감기전

최종원,박희준,이경태,박건영,한갑이,정민화 한국생명과학회 2002 생명과학회지 Vol.12 No.3

옻나무(Rhus verniciflua Stokes)로부터 간섬유화에 따른 간독성의 경감기전을 추구할 목적으로 ethyl acetate 분획에서 분리한 fustin 및 sulfuretin을 실험동물에 투여하고서 혈액학적 변화 및 간장 중 활성산소에 미치는 영향 검토한 결과 담도결찰하여 간섬유화를 유도한 군에서는 AST, ALT, SDH, ${\gamma}$-GT활성 및 total bilirubin의 양이 현저히 증가되던 것이 옻나무의 메탄을 엑스(250 mg/kg), 에틸아세테이트 엑스(250 mg/kg), fustin(10 mg/kg) 및 sulfuretin(10 mg/kg)을 2주간 각각 경구투여 하므로서 유의성 있게 억제되었다. Hydroxyproline양 및 MDA 농도에서도 간섬유화를 유도하므로서 현저히 증가되던 것이 옻나무의 분획 및 sulfuretin과 fustin의 투여로서 각각 약 60% 및 47%정도 감소되었다. 간섬유화를 유도한 cytosolic계 효소인 xanthine oxidase 및 aldehyde oxidase의 활성이 현저히 증가되었으며 한편 superoxide dismutase, glutathione peroxidase 및 catalase은 간섬유화의 유도로 감소되었으나 옻나무의 분획(메탄올, 에틸아세테이트) 및 성분(fustin, sulfuretin)의 투여로서 조절되었다. The pathogenesis of cholestatic liver injury as well as the modulation of hepatic fibrogenesis is causally associated with involvement of reactive oxygen species and free radical reactions. In this study, we investigated whether flavonoids (fustin, sulfuretin) which were isolated from Rhus verniciflua Stokes (RCS) have antioxidant and antihepatotoxicity effect under the biliary liver fibrosis condition. After surgery (control) and posttreated RCS methanol extract (250mg/kg), ethyl acetate extract (250mg/kg) and flavonoids were administered p.o. 10mg/kg/day in two weeks for control groups. The concentration of clinical parameters and product of hepatic lipid peroxidation and the hydroxyproline content were significantly increased in liver fibrosis developed rats. Among the clinical parameters of serum, value of ALT, AST, SDH, total bilirubin and ${\gamma}$ -GT in posttreated RCS components-group showed significantly lower than in control-group. The content of hydroxyproline in posttreated RCS components-group showed lower than in control group and then the value of MDA in posttreated RCS components-group was also significantly reduced to 40~60% of that in control group. The hepatic xanthine oxidase and aldehyde oxidate activities were posttreated RCS components-group showed significantly lower than in control-group. The hepatic SOD and glutathione peroxidase activities were posttreated RCS components-group showed significantly higher than in control-group. Hence we concluded that active components of fustin and sulfuretin which were isolated from R. verniciflua Stokes were hepatoprotective effect in experimental liver fibrosis.

오존처리에 의한 Bromate의 생성이 고환독성에 미치는 영향

이무강,정운용,정민화,한갑이,조희숙,최종원 慶星大學校 環境問題硏究所 2002 環境硏報 Vol.12 No.1

Bromate is a chemical oxidizing agent that has been used in analytical chemistry, in four milling, in beer malting, in cheese making, as a component of home and salon permanent hair-wave kits. In this study the objective was to investigate the effects of testis toxicity due to bromate in rats. On the biological study, the body weight changes, bromate(0, 0.1, 0.2, 0.4g/L) treated group showed decreased in the body weight and testis compared to the normal group. But kidney weight was increased. Hepatic and spleen weight were not effected compared to the normal group. Lipid peroxide content in the testis was increased by bromate which was concentrated 0.4g/L for 24 weeks. Testis cytosolic enzyme system(xanthine oxidase, aldehyde oxidase) of bromate group were significantly increased in comparison with those of normal group and microsomal enzyme system were not effected. And blood testosterone concentration was significantly decreased in comparison with those of group given bromate.

식이성 고지혈증에 미치는 숙지황의 영향 및 항산화활성에 관한 연구

이용범,조희숙,박정관,한갑이,신명희,최종원 慶星大學校 環境問題硏究所 2001 環境硏報 Vol.11 No.1

Shug Di Huang, Rehmanniae Radix, is the fresh or dry root of Rehmannia glutinosa Libosch.(Scrophulariaceae). In this study, we investigated the effect of Rehmanniae Radix Preparata on the dietary hyperlipidemia, blood coagulation system and antioxidant effect. In the dietary hyperlipidemia induced rat, it was observed that serum triglyceride, phospholipid, total, VLDL, LDL-cholesterol level and atherosclerotic index were higher, while the HDL-cholesterol level was lower when compared to the normal group. However, these changes were prevented by Shug Di Huang treatment. Activity of hepatic HMG-CoA reductase was not changed in dietary hyperlipidemia induced rats. In dietary hyperlipidemia caused, the decreased of bleeding time and increased of tissue factor activity were regluated by Shug Di Huang treatment. In the dietary hyperlipidemia induced group, the serum superoxide dismutase activity decreased and formation of hydroxyl radical increased when compared to normal, but these were controlled by Shug Di Huang treatment. In dietary hyperlipidemia caused, the increased of hepatic lipid peroxide content was inhibited by treatment with Shug Di Huang and dietary hyperlipidemia decreased the hepatic glutathione compared to normal group, but these were inhibited by Shug Di Huang treatment. There were a significant rise in hepatic microsomal aminopyrine N-demethylase and cytosolic xanthine oxidase activities, but these were controlled by Shug Di Huang treatment. Hepatic superoxide dismutase, catalase and glutathione peroxidase were significantly rised by treatment with Shug Di Huang when compare hyperlipidemic rats. But glutathione reductase and γ -glutamylcystein synthetase were not changes in dietary hyperlipidemic rats. Therefore, it can be concluded that Shug Di Huang treatment suppresses dietaty hyperlipidemia induced changes in serum lipid composition, hepatic metabolizing system and it has anti-oxidant effects.