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반추위 총 미생물 , Bacteria 및 Protozoa 의 화학적 조성과 Pepsin 소화율
최충국,문여황 한국낙농학회 1994 韓國酪農學會誌 Vol.16 No.4
반추위 누관이 시술된 면양 2두로 부터 사료급여후 시간별로 채취, 혼합한 위액중 반추위 총미생물, bacteria 및 protozoa를 각각 분리하여 영양소 함량과 pepsin소화율을 조사한 결과는 다음과 같다. 1. 반추위 총 미생물의 건물회수량은 위액 100㎖당 0.51g이었고, bacteria와 protozoa의 건물비율은 65 : 35로서 bacteria가 많았다. 2. 조단백질 함량은 총 미생물, bacteria 및 protozoa가 각각 37.3%, 40.2% 및 30.5%로서 bacteria가 가장 높았다. 3. 조지방 함량은 각 미생물이 12.5%∼13.1% 범위로서 비슷한 수준이었다. 4. 조회분 함량은 총 미생물, bacteria 및 protozoa가 각각 15.4%, 18.9% 및 12.13%로서 bacteria가 가장 높았다. 5. 반추위 미생물 건물의 pepsin 소화율은 41.0%∼43.8%로서 비슷한 수준이었으나 조단백질 소화율은 총 미생물, bacteria 및 protozoa가 각각 88.4%, 81.3% 및 93.5%로서 protozoa가 가장 높았다. 6. 아미노산 조성은 각 미생물간에 비슷한 수준이 었으며, 필수아미노산 중 leusine과 lysine의 함량이 높았다. Methionine함량은 아미노산 100g중 1.71∼3.23g으로서 필수아미노산 중에서는 가장 낮았으나 반추위 미생물 중에서는 박테리아가 가장 함량이 높았다. This experiment was conducted to investigate the nutrient content and pepsin digestibility of ruminal total microbes, bacteria and protozoa isolated from rumen fluid of two ruminally cannulated sheep fed 50 : 50 of concentrates and rice straw with a 130% requirement for maintenance. 1. Total microbial dry matter was recovered by 0.51g per 100 ㎖ rumen fluid, and ratio of protozoa to bacteria weight was 35 : 65. 2. Crude protein contents of ruminal total microbes, bacteria and protozoa were 37.3%, 40.2% and 30.5%, respectively. 3. Crude fat contents of three ruminal microorganisms ranged from 12.5% to 13.1%. 4. Crude ash contents were 15.4% for total microbes, 18.9% for bacteria and 12.13% for protozoa. 5. Pepsin digestibility of microbial dry matter was similar (41.0∼43.8%) among three ruminal microorganisms, but those of microbial crude protein were 88.4% for total microbes, 81.3% for bacteria and 93.5% for protozoa. 6. Composition of amino acid had a similar profile among three ruminal microorganisms, and contents of leusine and lysine were highest. Content of methionine which was the lowest of rumen microbial essential amino acids was ranged from 1.71g to 3.23g per 100g amino acid.
감소된 치조골 고경을 갖는 치아와 골유착성 임프랜트에 의해 지지되는 고정성 국소의치의 유한요소법적 응력분석
최충국,계기성,조규종,Choi Choong-Kug,Kay Kee-Sung,Cho Kyu-Zong 대한치과보철학회 1994 대한치과보철학회지 Vol.32 No.2
The purpose of this study was to evaluate the mechanical effects when one implant fixture was connected to the natural teeth with reduced alveolar bone height. This study also examined the effects of increasing the number of abutment teeth and the effects of the intramobile connector and the titanium connector as they were inserted between the implant superstructure and the fixture. The distribution and concentration load was applied to the fixed partial denture(FPD) supported by implant and the natural teeth with reduced alveolar bone height. The stress and displacement of each element was observed and compared by the two-dimensional finite element method. The following results were obtained : 1. The greater the loss of alveolar bone in natural teeth area, the greater the displacement of FPD and the stress concentration in alveolar bone around implant, especially at the stress concentration in the mesial alveolar bone crest around implant fixture. 2. The displacement of FPD was increased more and that of implants fixture was decreased more when intramobile connector was used than titanium connector was used. Also the stress concentration in alveolar bone around implant fixture was greater when intramobile connector than titanium connector. One implication of this finding was that the difference in stiffness of implant and the natural teeth with reduced alveolar bone height could be partially compensated in case of the POM intramobile connector. 3. The amount and direction of displacement and the stress distribution of the 4-unit FPD was better than those of the 3-unit FPD. It implied that the difference of stiffness of implant and natural teeth with reduced alveolar bone height could be partially compensated in case of the 4 unit FPD.
젖산구균에 있어서 접합에 의한 plasmids 의 전이에 관한 연구
최충국 한국낙농학회 1989 韓國酪農學會誌 Vol.11 No.4
이 연구는 젖산구균에 있어서 plasmids의 조성, 기능 및 접합에 의한 전이 특성을 조사하기 위해 수행되었다. Curing 및 접합실험에 의해 Streptococcus lactis 527이 함유하는 32Mdal plasmic는 유당발효성에 관련된 plasmid로 밝혀졌고, S. faecalis DS5에서 유래한 plasmid pAMβ1이 젖산구균의 Plasmid를 mobilize 할 가능성이 있음을 추측할 수 있었다. This study was carried out to investigate the composition, function, and conjugal transfer of plasmid in lactic streptococci. Curing and agar surface mating revealed that a 32 Mdal plasmid of Streptococcus lactis 527 was linked to lactose fermenting ability and that the possibility of mobilizing lactic streptococcal plasmids by a streptococcal plasmid pAMβ1 may exist.
Vita Lumin Shade Guide와 Vita Shade Resin Composite 간의 색깔차이
고영무,최한철,최충국 대한치과기재학회 1996 대한치과재료학회지 Vol.23 No.2
The Purpose of this study was to evaluate the possibility of using Vita Lumin shade guide to select shades of composite resin. Vita Lumin shade guide, such as four kinds of composite resin; Z100(3M), Prisma T.P.H.(Caulk/Dentsply), Tetric(Vivadent), Dentacolor resin system(Kulzer Co.) and shade guide which is supplied by the manufacturer were used in this study. The four kinds of shade which are poplar were used; A2,A3,B3, and D3, but, in case of Prisma T.P.H. we chose A3.5 instead of A3 because it does not have A3 shade. The shade guide from the manufacturer and Vita Lumin shade guide are ready made but we made 6 samples od each composite resin which size are 10±1mm in diameter, 2.0±0.1mm in thickness, and then composite resin were light cured for 40 sec. In case of the Dentacolor resin system, we put them into the Dentacolor XS unit then light cured 180 sec. according to manufacturer's instruction. After the curing, we eliminated the samples from the molds and stored them in distilled water for 48 hours. Using the Colorimeter(Hunter Inc, USA) and examining the value of L*a*b* for three times, we calculated their mean values. The resulte were as follows; among all 4 kinds of composite resin which are produced by same shade, shade guide which are supplied by same shade vs. composite resin, there are big differences more than expected. In the view of the result of this study, there is a little problem with the use of the Vita Lumin shade guide and the shade guide which is supplied by munufacturer. Therefore, we conclude that we need more accurate the cuatomized shade guide. This study was supported by research funds from Chosun university.
Streptococcus faecalis의 細胞凝集抗原과 그 Plasmid에 關한 硏究
崔忠國,金顯旭 서울大學校 農科大學 1986 서울대농학연구지 Vol.11 No.1
Several strains of Steptococcus faecalis have been studied for the cell agglutination and their plasmids, including antibiotics resistance. The results obtained were summarized as follows. 1. Cell aggregation took place in four pairs of culture mixtures including S. faecalis DS-5 and the aggregation response was strongest between S. faecalis DS-5 and S. faecalis ATCC 29200. 2. S. faecalis DS-5 and S. faecalis ATCC 29200 were more resistant to several kinds of antibiotics than other strains tested. Antibiotes resistance of S. faecalis DS-5 was not cured by acridine orange treatment. 3. S. faecalis ATCC 19953 happened to have no plasmid and other strains of S. faecalis had one to four plasmids.