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RISS 인기검색어
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- 저자 : 최원경(Won Hyung Choi) (검색결과 4 건)
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녹차의 에탄올 추출물이 암세포주의 형태학적변화에 마치는 영향
장원태 ( Won Tae Jang ),이효권 ( Hyo Kwon Lee ),김미형 ( Mi Hyung Kim ),류인 ( Yin Liu ),최원경 ( Won Kyung Choi ),성훈기 ( Hoon Ki Sung ),박정현 ( Jeong Hyun Park ) 대한임상검사과학회 2000 대한임상검사과학회지(KJCLS) Vol.32 No.3
Green tea is one of most popular traditional teas in korea. ``The purpose of this study was comparing the effects of ethanol extracts on three kinds of cancer cell lines(HepG2, A549, EATC) according to its concentration and exposure time. For this purpose, we obseπed morphological changes under inverted and electron microscope and anallysed cytotoxicity . EATC(Ehrlich-Lettre Ascites Tumor Cell) cells were damaged by 100μg/m1 ethanol extracts treatment until 72 hours gradually. In A549 cells, ethanol extracts caused severe cellular injury abrupt1y and lasted for 72 hours. However, cellular necrosis and growth inhibition in both of them could not be observed in 1, 10μg/ml ethanol extracts administration. Whereas, the effects of that were weak in all of concentrations and exposure times in HepG2 cells. In conclusion, green tea ethan이 extracts induced strong inhibition of growth and severe cellular damages in A549(Lung Cancer) and EATC except HepG2(Hepatoblastoma). In addition, its effects were dependent on concentration and duration of ethanol extracts. Therefore, though there were some different effect according to cancer type, it suggested that green tea would be helpful to prevent carcinogenesis and treat cancer.
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열화학기상증착법에 의한 탄소나노소재의 합성 및 수소저장 특성
유형균,최원경,류호진,이병일,Yu, Hyung-Kyun,Choi, Won-Kyung,Ryu, Ho-Jin,Lee, Byung-Il 한국세라믹학회 2001 한국세라믹학회지 Vol.38 No.10
지지촉매로 Ni-graphite를 사용하고 ${C_2}{H_2}$를 이용한 열화학기상증착법에 의하여 탄소나노소재를 합성하였다. 합성된 시편은 SEM, TEM, 라만 분광법으로 분석하였으며, 수소저장특성은 전기화학적인 방법에 의하여 평가하였다. 탄소나노소재 합성시 메카노케미컬 처리과정을 거친 시편이 거치지 않은 시편에 비하여 탄소나노튜브의 순도가 우수하였다. 한편, 탄소나노소재를 정제함에 따라 수소저장특성이 크게 향상되었다. The carbon nanomaterials were prepared using the thermal chemical vapor deposition with ${C_2}{H_2}$ on the Ni-graphite mixture as a supported catalyst. The samples were identified by SEM, TEM, Raman spectroscopy, and the hydrogen storage measurement by electrochemical method was also carried out. The purity of carbon nanotube prepared using ground mixture was higher than that of unground one. Also, the amount of hydrogen storage of purified carbon nanomaterials was more than that of unpurified one.
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최원경,최병재,최형준,이종갑 大韓小兒齒科學會 1999 大韓小兒齒科學會誌 Vol.26 No.2
치아탈구 발생시 사고 현장에서 쉽게 구할 수 있는 저장용액을 이용하여 치주인대세포의 생존율을 비교해보고자 치주인대세포를 10%FBS함유 α-MEM에서 배양한 후 α-MEM에서 저장한 군을 대조군으로 하고 동일한 조건하에서 저온살균된 우유군, 멸균된 생리적 식염수군, 비자극성 타액군, 건조된 상태로 방치한 군을 실험군으로 하여 25℃ 실온에서 세포 배양 30, 60, 90, 120, 180분 후에 각 군에 대해 MTTassay를 시행하여 다음과 같은 결론을 얻었다. 1. 각 조사시점에서 실험군간의 비교에서는 모두 유의성있는 차이를 보였으며 저온살균된 우유군에서 치주인대세포의 생존율이 가장 높았고, 멸균된 생리적 식염수군, 비자극성 타액군, 건조된 상태로 방치한 군 순으로 점차 감소하였다.(P<0.05) 2. 각 실험군내에서 조사시점간의 비교에서는 치주인대세포의 생존율이 시간이 경과함에 따라 유의성있게 감소하였으나(p<0.05). 저온살균된 우유군에서 90-120분 경과 사이. 멸균된 생리적 식염수군에서 60-90분과 120-180분 경과 사이에서는 유의성있는 차이는 없었다.(P>0.05) 이상의 결론을 종합해볼 때 외상으로 인한 치아탈구 발생시 저장용액으로 HBSS가 추천되고 있으나 사고 현장에서 쉽게 구할 수 있고 치주인대세포의 생활력 보존에도 유리한 저온살균된 우유에 탈구된 치아를 담가오도록 추천할 수 있겠다. Preservation of the remaining periodontal ligament cells on an avulsed tooth is very important to the successful outcome of replantation. HBSS is recommended as the most suitable storage medium for the avulsed tooth that cannot be replanted immediately. But their availability near the site of an accident is doubtful. The purpose of this in vitro study was to compare periodontal ligament cells stored in different storage media obtained easily on th spot. Human periodontal ligament cells were collected from the premolar teeth extracted for orthodontic treatment. Cells were cultured in α-MEM culture medium containing 20% FBS, at 37℃ 100% humidity, in a 5% CO₂2 incubator. Cells were cultured in 96 well culture plate, 5×10⁴cells per well with α-MEM and incubated for 24 hours.After discarding the medium, those cells were cultured in α-MEM contained with 10% FBS, pasteurized milk, sterilized saline, unstimulated saliva and bench-dried state at 25℃ room temperature for 30, 60, 90, 120, 180 minutes respectively. And then each group was measured using MTT assay. The results were as follows. 1. Between the group of each time, there was statistically significant difference. Periodontal ligament cells viability was highest in pasteurized milk and was reduced stepwisely in sterilized saline, unstimulated saliva and bench-dried state(p<0.05) 2. between the time of each group, there was statistically significant difference (p<0.05) but was no statistically significant difference at 90 - 120 minutes in pasteurized milk and at 60 - 90 minutes and 120 - 180 minutes in sterilized saline(p>0.05). In conclusion, HBSS as storage medium of an avulsed tooth is not practical on the spot. Insteadily pasteurized milk can be recommended to maintain the periodontal ligament cells viability.
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