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人蔘根腐病原菌 Pseudomonas panacis 와 P. fluorescens 의 營養要求性에 관하여
李殷永,崔度鏡 全北大學校 學徒護國團 1977 全國大學生學術硏究發表論文集 Vol.1 No.-
This study was carried on to elucidate the requirement of nutrients of two strain of Pseudomonas panacis(P 11) and Pseudomonas fluorescens(P 12). 1. The optimum pH of these two strain wa 6.5. 2. In the carbon sources as a nutrients, sucrose, glucose and fructose were good growth factor in these two strain. Organic acid and poly or hexahydric alcohol were not nearly used as a nutritional sources. 3. In the effect of nitrogen sources on the growth of these two strain, asparagine as organic nitrogen was most effective, but not in methionine and isoleucine. KNO_3 as inorgamic nitrogen was useful, but not in KNO_2 and NaNo_2. 4. The optimum phosphate concentration in KH_2PO_4 and K_2HPO_4 were 0.01g/100ml of culture solution, and CaHPO_4, Na_3PO_4, CaH_4(PO_4)_2·H_2O, and FePO_4·4H_2O were 0.1g/100㎖ of culture solution. 5. Concentration of micrometallic element as growth factor of these two strain was nearby 0.1mg/100㎖ of culture solution, and among the elements the effective sources were FeCl_3, FeSO_4, MnSO_4 and MgCl_2. 6. New composition medium for the optimum growth of P Ⅱ strain based on the above results was as follow; asparagine 1.0g, KH_2PO_4, 0.1g, sucrose 30.0g, MgSo_4 0.5g, FeCl_3 1mg, thiamine HCl 200r and distilled water 1,000㎖, and P 12 strain was same with P Ⅱ strain except for sucrose substitute by glucose 30.0g.