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Cloing and Expression of Bdi Ⅰ Methylase Gene in E . coli
노현모,김용석,최경래 한국유전학회 1984 Genes & Genomics Vol.6 No.3
The gene for the Bdi I modification enzyme, which is a counterpart of Bdi I restriction endonuclease, from Brevibacterium divaricatum FERM 5948 was cloned and expressed in E. coli. First, we have isolated Bdi I restriction endonuclease by cleavage assay in vitro with three column steps and have proved that Bdi I restriction enzyme is an isoschizomer of Cla I whose recognition sequence is 5' ATCGAT 3'. For cloning of Bdi I methylase gene, we have initially used three cloning sites (Sal I, Bam HI and Eco RI) of pBR 322 and adopted the retransformation method after Bdi I restriction endonuclease cleavage. Selection of transformants carrying the gene was based on the resistance of the modified plasmid encoding the enzyme to cleavage by Bdi I restriction enzyme. Among colonies after retransformation, the recombinant plasmid, pBDIM 116 containing 5.2 Kb EcoRI insert was proved to carry the gene. Using the restriction map constructed, we inserted λ-Sal I fragment containing another Bdi I site into pBDIM 116 and subcloned various DNA fragment for Bdi I methylase gene mapping. The expression of Bdi I methylase gene in E. coli and the relationship between Bdi I restriction-modification genes will be also discussed.
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대장균 내에서의 Bdi I Methylase 유전자의 클로닝과 발현
전희숙,김용석,최경래,노현모 한국미생물학회 1987 미생물학회지 Vol.25 No.1
B Brevibacterium divaricatum FERM 5948 균주로부터 Bdi I RIM 체계에 속하는 BdiI methylase 유천자를 클로닝하여 발현을 조사하였다. Bdi I methylase 유전자의 클로닝을 위해 pBR 322의 EcoRI, BamHl, Sal I 3 군데의 클로닝 site를 이 용했고 1 차 형질전환후 나온 플라스미드를 BdiI으로 자른 뒤 ligation 시키지 않고 형질전환시키는 방법을 이용하였다. 유전 자을 가지는 행질전환체의 선별은 Bdi I methylase에 의해 수정된 채조합 플라스미드는 BdiI 제한효소에 방호된다는 것에 기 초하여 선별하였는데 5.6kb의 EcoRI insert DNA를 가지는 pBDIM 116이 Bdil methylase 유전자플 가지는 것으로 판명 되었다. pBDIM 11&을 가지는 숙주셰포에서 추출한 추출용액에는 S-adenosylmethionine이 있으면 BdiI의 인지부위인 A ATCGAT에만 특정한 methylase 활성이 측정되였다. 11개의 제한효소를 이용하이 제한효소지도를 작성하였고, BdiI r restriction -modification 체계에 관해서 도 논의하였다. The gene for the Bdi I modification enzyme, which is one of Bdi I restriction-modification system, fromBrevibacterium divaricatum FERM 5948 was cloned and expressed in E. coli. For cloning of the Bdi I methylase gene, we have initially used three cloning site(EcoRI, BamHI and Sal I) of plasmid vector pBR 322 and adopted the retransformation method after Bdi I restriction endonuclease cleavage. Selection of transformants carrying the gene was based on the resistance of the modified plasmid encoding the enzyme to cleavage by Bdi I restriction enzyme, and the recombinant plasmid pBDIM 116 containing 5.6kb EcoRI insery was proved to carry the gene. Crude cell extracts prepared from strains carrying the plasmid pBDIM 116 contained an S-adenosylmethionine-dependent methyltransferase activity specific for the Bdi I recognition site, ATCGAT. The restriction map was constructed with 11 restriction enzyme, and the Bdi I restriction-modification system was also discussed.
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인청동 스위칭 모듈을 이용한 전력계통 및 전자기기 내부회로의 MOV 열폭주 방지와 안전성 개선
김주철(Ju-Chul Kim),최경래(Gyung-Ray Choi),이상중(Sang-Joong Lee) 한국조명·전기설비학회 2011 조명·전기설비학회논문지 Vol.25 No.9
The MOV(Metal Oxide Varistor), a voltage limiting element, has been installed in the SPD(Surge Protective Device) or inside the internal circuit of an electronic appliance for protection of the electric power system and electronic device against electrical surge. Such an MOV is exposed, however, to the risk of the thermal runaway resulting from excessive voltage and deterioration. In this paper, a reciprocal action has been tested and analyzed using a phosphorus bronze switching module and the low-temperature solder. And a short current break characteristic test linked with the circuit breaker has been performed to limit the inrush current when the MOV breaks down. It has been proven that the phosphorus bronze switching module installed inside the internal circuit can improve the safety of the power line system and the electronic device.
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내장 순시 트립장치를 이용한 MOV의 열폭주 보호와 SPD의 안전성 개선
김주철(Ju-Chul Kim),전주술(Joo-Sool Jeon),기채옥(Che-Ouk Ki),최경래(Gyung-Ray Choi),이상중(Sang-Joong Lee) 한국조명·전기설비학회 2011 조명·전기설비학회논문지 Vol.25 No.2
SPDs are increasingly being used against lightning and switching surge according to the applicable revised standard and equipotential grounding system. SPDs are equipped usually with a MOV voltage regulating element. The MOV, however, always is exposed to the danger of thermal runaway resulting from inrushing temporary overvoltage and deterioration. In this paper, the authors made two prototype SPDs built-in Instantaneous trip device and analyzed their limiting voltage through test of the MOV breakdown. As the result of the analysis, the SPDs built-in Instantaneous trip device was proven to be effective for protecting MOV against thermal runaway and Instantaneous trip device react for limiting voltage is considered that is applicable to SPD.
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