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- 저자 : 채영아 (검색결과 3 건)
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니켈-크롬 합금 보철물 주위 치은열구 내에서 발견된 니켈 내성 균주에 관한 분자생물학적 연구
채영아,우이형,최부병,최대균,이성복,권긍록,Chae, Young-Ah,Woo, Yi-Hyung,Choi, Boo-Byung,Choi, Dae-Gyun,Lee, Sung-Bok,Kwon, Kung-Rock 대한치과보철학회 1999 대한치과보철학회지 Vol.37 No.6
As a material of metal-ceramic prosthesis, nickel as a form of Ni-Cr alloy has been used for many dental prostheses in many cases. However, several problems in use of the alloy have been revealed (ex : tissue stimulation, skin allergy, hypersensitivity, cytotoxicity and carcinogenecity). Little is known about nickel with respect to the relationship between Ni-prosthesis and gaining of Ni-resistance in oral microorganisms. The present study was undertaken to check wheather use of Ni-prosthesis leads to occurrence of Ni-resistant microorganisms. So this study may suggest the possible relationships between the oral microorganisms and nickel-resistance in oral environment. Bacteria were isolated from the gingival crevicular fluid on the pateints wearing Ni-Cr prosthesis. The isolated bacteria were tested for their Ni-resistance in nickel containing media at different concentration from 3mM to 110mM. E. coli HB101 was used as control. The Ni-resistant bacteria were isolated and biochemically identified. The Ni-resistant bacteria were tested several bio-chemical, molecular-biological tests. Performed tests were ; measuring the growth curve, antibiotic test, growth ability test in liquid media, isolation of the chromosome and plasmid, digestion of DNA by restriction enzyme, electrophoresis of chromosome and plasmid DNA, identification of Ni-resistant genes by the DNA hybridization. The results were as follows: 1) The bacteria isolated from gingival crevicular fluid on the patients wearing Ni-Cr alloy pros-thesis showed nickel-resistance. 2) The isolated microorganisms grew at nickel containing media of high concentrations (60mM-110mM). 3) Based on the biochemical tests, the isolated microorganisms were identified as Enterococcus faecalis(13 cases), Klebsiella pneumoniae(1 case) and Enterobacter gergeviae(1 case). 4) Enterococcus faecalis expressed not only nickel resistance but also the multi-drug resistance to several antibiotics ; chloramphenicol, kanamicin, streptomycin, lincomycin, clindamycin. However, all strain showed the sensitivity against the tetracycline. 5) DNA hybridization result suggest that there is no homology between the previousely known gene of nickel resistance in Klebsiella pneumoniae and chromosomal DNA of Enterococcus faecalis.
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니켈-크롬 합금 보철물 주위 치은 열구내에서 발견된 니켈 내성 균주에 관한 분자생물학적 연구
채영아,우이형,이성복,Chae Young-Ah,Woo Yi-Hyung,Kwon Kung-Rock 대한치과보철학회 2003 대한치과보철학회지 Vol.41 No.2
As a material of metal-ceramic prosthesis, nickel as a form of Ni-Cr alloy has been used for many dental prostheses in many cases. However, several problems in use of the alloy have been revealed (ex ; tissue stimulation, skin allergy, hypersensitivity cytotoxicity and carcinogenecity). Little is known about nickel with respect to the relationship between Ni-prosthesis and gaining of Niresistance in oral microorganisms. The present study was undertaken to check whether use of Ni-prosthesis leads to occurrence of Ni-resistant microorganisms. So this study may suggest the possible relationships between the oral microorganisms and nickel-resistance in oral environment. Bacteria were isolated from the gingival crevicular fluid on the patients wearing Ni-Cr prosthesis. The isolated bacteria were tested fir their Ni-resistance in nickel containing media at different concentration from 3mM to 110mM. E. coli HB101 was used as control. The Ni-resistant bacteria were isolated and biochemically identified. The Ni-resistant bacteria were tested several biochemical, molecular-biological tests. Performed tests were : measuring the growth curve, antibiotic test, growth ability test in liquid media, isolation of the chromosome and plasmid, digestion of DNA by restriction enzyme, electrophoresis of chromosome and plasmid DNA, identification of Ni-resistant genes by the DNA hybridization. The results were as follows 1) The bacteria isolated from gingival crevicular fluid on the patients wearing Ni-Cr alloy prosthesis showed nickel-resistance. 2) The isolated microorganisms grew at nickel containing media of high concentrations (60mM-110mM). 3) Based on the biochemical tests, the isolated microorganisms were identified as Enterococcus faecalis(13 cases), Klebsiella pneumoniae(1 case) and Enterobacter gergoviae(1 case). 4) Enterococcus faecalis expressed not only nickel resistance but also the multi-drug resistance to several antibiotics ; chloramphenicol, kanamicin, streptomycin, lincomycin, clindamycin, However, all strain showed the sensitivity against the tetracycline. 5) DNA hybridization result suggests that there is no homology between the previously known gene of nickel resistance in Klebsiella pneumoniae and chromosomal DNA of Enterococcus faecalis.
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T-scan을 이용한 성인정상교합자의 하악운동시 치아접촉양상에 관한 연구
채영아,박남수,최부병,Chai Young-Ah,Park Nam-Soo,Choi Boo-Byung 대한치과보철학회 1993 대한치과보철학회지 Vol.31 No.4
This study was accomplished to analyse and compare the occlusal contact patterns during eccentric mandibular movements in adult with normal occlusion. 50 subjects(male 27, female 23), who had natural occlusion and no symptom of temporomandibular disorder, were selected. Teeth contact patterns during mandibular eccentric movements were recorded and the distribution of tooth contacts in maximum intercuspation analysed by T-scan system. And then, tooth contact numbers recored by T-scan and silicone bite registration at centric occlusion were analysed and compared. The results obtained were as follows : 1. Antero-posteriorly, the qualitative center of occlusal contacts in centric occlusion were in the first molar areas, but there was a slight deviation in left-right directions. Thus, distribution of occlusal contacts were not bilaterally symmetric. 2. During the mandibular movements from centric occlusal position to right lateral and left lateral directions, the frequency that maxillary canine joined in lateral guidance was relatively high, but pure canine protected occlusion or pure group function occlusion had small frequency. 3. During mandibular protrusive movement, one or more maxillary central incisors frequently joined in protrusive guidance. 4. During mandibular eccentric movements, working and balancing side premature contact was observered in relatively high frequency. 5. In centric occlusal position, the numbers of occlusal contacts recorded on T-scan were relatively smaller than on silicone bite registration.
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