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신장 허혈 재관류 손상에 미치는 Ethyl Pyruvate의 효과
정구용(Ku-Yong Chung),정규영(Gyu Young Jeong),안형준(Hyung Joon Ahn),주만기(Man Ki Ju),장혜경(Hye Kyung Chang),이우정(Woo Jung Lee),한평림(Pyung-Lim Han),김유선(Yu Seun Kim) 대한외과학회 2007 Annals of Surgical Treatment and Research(ASRT) Vol.72 No.5
Purpose: Reactive oxygen species (ROS) significantly contribute to ischemia-reperfusion injury, and are also associated with the gradual loss of renal function and renal failure following renal transplantation. Pyruvate is an endogenous antioxidant, but its use as a therapeutic agent for treating conditions mediated by oxidative stress is limited due to its poor stability in solution. However, ethyl pyruvate (EP), a soluble pyruvate derivative, has far greater stability than pyruvate; thus, may serve as a practical pyruvate precursor. Therefore, the ability of EP in the prevention of renal ischemia- reperfusion injury was assessed. Methods: Sprague-Dawley rats (n=54) were subjected to 40 minutes of renal warm ischemia. The animals were divided into three groups: the sham group without warm ischemia (n=18), the EP group (n=18, EP given before ischemia), and the ischemic control (n=18). The serum levels of creatinine and TNF-α were measured 1, 3 and 5 days after induction of ischemia. The expression of high mobility group box-1 (HMGB-1), a delayed inflammatory mediator, was also assessed by Western blot of renal specimens. Results: In the EP group, late improvements in the serum levels of creatinine and TNF-α were observed in comparison with the ischemic control. Based on this delayed effect, the expression of HMGB-1 was assessed in renal tissue. The HMGB-1 expression increased over time during the ischemia process, but EP suppressed this expression 3 and 5 days after renal ischemia-reperfusion injury. Conclusion: These results have demonstrated, for the first time, that EP ameliorates renal ischemia-reperfusion injury. EP attenuates the renal ischemia-reperfusion injury, at least in part, by suppressing the expression of HMGB-1, a late mediator of delayed inflammation.
Mycophenolic Acid가 올레산으로 유도한 흰쥐 혈관 평활근 세포의 증식에 미치는 영향
안형준(Hyung Joon Ahn),박제현(Jehyun Park),송재숙(Jae Sook Song),주만기(Man Ki Ju),김명수(Myoung Soo Kim),하헌주(Hunjoo Ha),송기호(Ki Ho Song),김유선(Yu Seun Kim) 대한외과학회 2007 Annals of Surgical Treatment and Research(ASRT) Vol.72 No.3
Purpose: Vascular smooth muscle cell (VSMC) proliferation plays an important role in the development and progression of chronic allograft vasculopathy. Mycophenolic acid (MPA) inhibits various mesenchymal cell proliferation, and reactive oxygen species (ROS) are involved in the anti-pro-liferative effect of MPA. In this study, we investigated the effects of MPA on oleic acid (OA)-induced VSMC proliferation and also the role of ROS in these processes. Methods: Primary cultured rat VSMCs from Sprague-Dawley were stimulated with OA 100μM. MPA 0.1∼10μM and N-acetylcystein (NAC) 5 mM were administered 1 hour before adding the OA. Cell proliferation was measured by Methylthiazoletetrazolium (MTT) assay, proliferating cell nuclear antigen (PCNA) expression by Western blot analysis, and dichlorofluorescein (DCF)-sensitive cellular ROS by flow cytometry. Results: OA at 100μM significantly increased MTT level by 1.6-fold as well as PCNA expression at 48 hours in rat VSMCs. OA also induced DCF-sensitive cellular ROS by 1.6-fold at 5 minutes and the increment of cellular ROS remained for up to 1 hour. MPA at above 1μM inhibited OA- induced VSMC proliferation and cellular ROS in a dosedependent manner. NAC 5 mM also inhibited OA-induced rat VSMC activation. Conclusion: These results suggest that MPA inhibits OA-induced VSMC proliferation partially through the inhibition of cellular ROS.
수상 후 발생한 골반강내 출혈에 대한 혈관조영 동맥색전술 치료효과
강성준,김영주,배금석,주만기 대한외상학회 2001 大韓外傷學會誌 Vol.14 No.2
Purpose: For pelvic bone fractures accompanying pelvic cavity hemorrhages in multiple trauma patients, surgical therapy and adjuvant therapy do not have sufficient effect on hemostasis. We studied the therapeutic effects of arterial embolization in patients with pelvic cavity hemorrhages. Method: The study was performed on eight male patients who had traffic accidents and who were treated with arterial embolization from March to July in 1999 at Wonju Christian Hospital based on abdominal-pelvic CT by using the Seldinger method and angiographies. After that, as a first choice internal iliac arterial embolization was performed on the vessels suggesting hemorrhage then the area was embolized with Gelfoam (1×1mm), Vasopressin infusion or stainless coils. For judging the therapeutic effects of arterial embolization, we use the shock index (S.I: heart rate/day) and the injected concentrated RBC amount. Result: Five of the eight patients had organ injuries in the abdominal cavity and received operations immediately; they had no evidence of hemorrhage outside the pelvic cavity. Three had no accompanying injuries. Gelfoam was used a five of the eight, A stainless coil was used with two patients for embolization infusion with one the patients' shock index and injected concentrated RBC amount after the procedure were 0.68±0.30 and 0.63±0.74, respectively statistically showing a significant reduction compared with the data before the procedure of 1.11±0.15 and 4.5±1.3 (p$lt;0.005), respectively. Conclusion: Pelvic bone injuries accompanying pelvic cavity hemorrhages can be treated effectively by arterial embolization through angiography or arterial Vasopressin infusion.