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비천연 아미노산이 도입된 재조합 요산분해효소의 생산을 위한 배양 최적화
조정행(Jeong-Haeng Cho),박성주(Seongju Park),최종일(Jong-il Choi) 한국생물공학회 2020 KSBB Journal Vol.35 No.1
In this study, the medium composition, carbon source, non-natural amino acid concentration, and induction condition were optimized for the production of recombinant urate oxidase with non-natural amino acids. Recombinant Escherichia coli strain was first cultured in various media including LB, 2 × YT and B, and cell growth and urate oxidase expression were compared. Based on the selected B medium, the concentrations of each component in medium were optimized through response surface methodology. At the optimal medium compositions, urate oxidase with non-natural amino acids was produced with 64.92 U/mg specific protein activity in flask culture. Among various carbon sources, the highest urate oxidase activity was obtained at the glucose concentration of 5 g/L. The optimal inducer concentrations were 1 mM of IPTG. 1 mM of non-natural amino acids was efficient for urate oxidase production. This results will be useful for the production of therapeutic proteins with prolonged activity.