부식된 하수관에서 분리한 곰팡이 OMSOf1 의 황화수소 제거 특성

조경숙,박상진 ( Kyung Suk Cho,Sang Jin Park ) 한국환경생물학회 1997 환경생물 : 환경생물학회지 Vol.15 No.2

A sulfide-oxidizing fungus was isolated from corroded concrete sewer pipes. From the physiological and morphological properties such as growth characteristics on diverse media, reproduction pattern, and size and shape of spores, the isolated fungus was considered as Sporormia sp. The fungus OMSOf1 could oxidize hydrogen sulfide (H_2S) to thiosulfate due to intracellular enzyme systems. The fungus was immobilized in polyurethane carriers which contained super absorbent polymer (sodium polyacrylic acid) permeated with mineral medium, and polyurethane. When 28ppm of H_2S was supplied to the biofilter packed with the carriers at 50㎖/min, more than 95% removal ratio of H_2S was obtained for 15 days without supply of water. The reduction of H_2S removal ratio by drying of the carriers could be recovered by supply of water.

Alcaligenes latus 의 배양방법에 따른 생분해성 플라스틱의 생산특성

조경숙,최희식,류희욱,조강현,박성연 ( Kyung Suk Cho,Hee Sik Choi,Hee Wook Ryu,Kang Hyun Cho,Seong Yeon Park ) 한국환경생물학회 1998 환경생물 : 환경생물학회지 Vol.16 No.1

Optimum conditions for cell growth and poly (3-hydroxybutyrate, PHB) biosynthesis of Alcaligenes latus. which can accumulate PHB simultaneously in associated with cell growth and grows well on a cheap carbon source such as sucrose, were investigated. The effect of initial sucrose concentration, ranged from 10 to 50g/L, on the cell growth and PHB accumulation were studied by a batch culture in a 5 L fermentor. PHB productivity was increased from 0.11 to 0. 36 g-PHB/L·h with increasing sucrose concentration up to 30g/L, and it was fairly constant between concentrations of 30g/L and 50g/L. The maximum specific growth rate, 0.44h^-1, was obtained at 30 g-sucrose/L, and the growth was inhibited at concentrations of sucrose over 30g/L. A. latus was also cultivated to attain high PHB productivity by fed-batch or semicontinuous fed-batch culture techniques. PHB productivity by a fed-batch culture under phosphate limitation (1.14g-PHB/L·h) were smaller than that without nutrient limitation (2.10g-PHB/L·h), but there is no significant difference of PHB content (55-60%) obtained in both culture. By a semicontinuous fed-batch culture without nutrient limitation, high PHB productivity could be obtained as 4.5∼4.8 g-PHB/L·h. The average PHB content, the yield of dry cell weight, and the yield of per consumed sucrose were 60%, 0.48, and 0.32, respectively. These results suggest that the semi continuous fed-batch culture technique is one of promising methods to achieve high PHB productivity by A. latus.

매립지의 메탄 배출 저감을 위한 생물공학기술

조경숙,류희욱,Cho, Kyung-Suk,Ryu, Hee-Wook 한국미생물·생명공학회 2009 한국미생물·생명공학회지 Vol.25 No.4

메탄은 온실효과가 이산화탄소 보다 20배 이상인 대표적인 non-$CO_2$ 온실가스이다. 매립지는 주요 인위적 메탄 발생원으로, 매립지의 메탄 발생량은 연간 35~73 Tg(tera gram)으로 추정된다. 바이오커버(개방형 시스템)과 바이오필터(폐쇄형 시스템)을 이용하는 생물학적 방법은 메탄을 회수하여 자원화하기에는 메탄 농도가 너무 낮거나 가스 포집정이 설치되어 있지 않는 노후화된 매립지나 소규모 매립지로부터 메탄 배출을 저감할 수 있는 유용한 방법이다. 메탄을 유일탄소원과 에너지원으로 활용하는 메탄산화세균은 이러한 생물학적 방법에 있어 메탄을 산화시켜 제거하는데 매우 중요한 역할을 담당한다. 토양, compost, 지렁이 분변토 등과 같은 다양한 충전재를 이용하여 실험실 규모의 바이오커버/바이오필터의 메탄산화효율에 관한 많은 연구가 진행되었다. 이 중에서 compost는 가장 많이 이용되고 있는 충전재이고, compost를 이용한 바이오커버/바이오필터의 메탄산화속도는 50에서 $700\;g-CH_4\;m^{-2}\;d^{-1}$로 보고되고 있다. 또한, 실제 매립지에 파일럿 규모의 바이오커버/바이오필터를 설치하여 메탄 배출 저감 효과에 관한 연구도 진행되고 있다. 매립지의 메탄 배출 저감은 탄소배출권 거래와 연관될 수 있으므로, 바이오커버/바이오필터에 의한 메탄 저감량을 정확하게 평가하는 것이 매우 중요하다. 그러므로, 매립지 현장에 설치된 바이오커버/바이오필터의 성능을 평가하는 방법은 표준화되어야 하며, 메탄 저감량을 정확하게 정량화할 수 있는 방법 개발이 필요하다. Methane, as a greenhouse gas, is some 21~25 times more detrimental to the environmental than carbon dioxide. Landfills generally constitute the most important anthropogenic source, and methane emission from landfill was estimated as 35~73 Tg per year. Biological approaches using biocover (open system) and biofilter (closed system) can be a promising solution for older and/or smaller landfills where the methane production is too low for energy recovery or flaring and installation of a gas extraction system is inefficient. Methanotrophic bacteria, utilizing methane as a sole carbon and energy source, are responsible for the aerobic degradation (oxidation) of methane in the biological systems. Many bench-scale studies have demonstrated a high oxidation capacity in diverse filter bed materials such as soil, compost, earthworm cast and etc. Compost had been most often employed in the biological systems, and the methane oxidation rates in compost biocovers/boifilters ranged from 50 to $700\;g-CH_4\;m^{-2}\;d^{-1}$. Some preliminary field trials have showed the suitability of biocovers/biofilters for practical application and their satisfactory performance in mitigation methane emissions. Since the reduction of landfill methane emissions has been linked to carbon credits and trading schemes, the verified quantification of mitigated emissions through biocovers/biofilters is very important. Therefore, the assessment of in situ biocovers/biofilters performance should be standardized, and the reliable quantification methods of methane reduction is necessary.

친환경 황토 보드를 이용한 경량 칸막이 벽체의 세대간 경계벽 적용을 위한 내화 및 차음 성능 연구

조경숙(Cho, Kyung-Suk),여인환(Yeo, In-Hwan) 대한건축학회 2013 大韓建築學會論文集 : 構造系 Vol.29 No.12

This study tested, in Long-Life Housing, partition wall between housing units which composite with middle lintel and Loess board, whether corresponded with requirements for performance criteria related with codes. It was get a performance level of Loess board that compared with gypsum board which used to partition wall components generally. It was tested requirements for fire resistance and sound insulation performance of partition wall in Korea Building Code. The results are, a insulation of the wall with gypsum board was better than Loess board, integrity of wall with loess board was better than gypsum board in fire. The sound insulation was, Loess board was better gypsum board due to porosity of loess and rice straw. Therefore, the partition wall between building units which composite with gypsum board on inside layer and Loess board on outside layer, with glass wool for insulation, is prefer to apply.

Gordona sp. MS6 균주의 dibenzothiophene 탈황능에 미치는 분해산물의 영향

조경숙(Kyung Suk Cho) 한국환경생물학회 2000 환경생물 : 환경생물학회지 Vol.18 No.1

Gordona sp. MS6, desulfurizing petroleum, can convert dibenzothiophene(DBT) to 2-hydroxybiphenyl(2-HBP) and sulfate. In this study, the effect of DBT degradation product on DBT desulfurization activity was investigated. When Na_2SO_4 and DBT were simultaneously added in the medium sulfur sources, specific growth rate of strain MS6 was doubled compared to not adding Na_2SO_4. But, sulfate inhibited DBT desulfurization rate, furthermore, when 0.05g/L Na_2SO_4 was supplied DBT desulfurization rate decreased down to 40%. When 2-HBP and its derivative, 2, 2´-dihydroxybiphenyl (DHBP) were not added, DBT desulfurization rate was 5.0 μmol·L^-1·h^-1. With the increase of 2-HBP and DHBP concentration, DBT desulfurization rate was decreased. No DBT desulfurization activity of Gordona sp. MS6 was observed when initial concentration was over 0.15 mM 2-HBP and 0.8 mM DHBP. [Gordona sp., Desulfurization, Dibenzothiophene, 2-Hydroxybiphenyl, Sulfate].

Fiber-cocktail을 혼입한 90MPa 강도 기둥의 강섬유 영향인자에 관한 실험적 연구

조경숙(Cho, Kyung Suk),김흥열(Kim, Heung Ryul),김형준(Kim, Hyung Jun),박경훈(Park, Kyung Hoon) 한국콘크리트학회 2010 한국콘크리트학회 학술대회 논문집 Vol.2010 No.5

Methanotrophs을 이용한 메탄 저감 기술 최신 동향

조경숙 ( Kyung-suk Cho ),정혜경 ( Hyekyeng Jung ) 한국미생물생명공학회 2017 한국미생물·생명공학회지 Vol.45 No.3

Methane, which is emitted from natural and anthropogenic sources, is a representative greenhouse gas for global warming. Methanotrophs are widespread in the environment and play an important role in the biological oxidation of methane via methane monooxygenases (MMOs), key enzymes for methane oxidation with broad substrate specificity. Methanotrophs have attracted attention as multifunctional bacteria with promising applications in biological methane mitigation technology and environmental bioremediation. In this review, we have summarized current knowledge regarding the biodiversity of methanotrophs, catalytic properties of MMOs, and high-cell density cultivation technology. In addition, we have reviewed the recent advances in biological methane mitigation technologies using methanotrophs in field-scale systems as well as in lab-scale bioreactors. We have also surveyed information on the dynamics of the methanotrophic community in biological systems and discussed the various challenges pertaining to methanotroph- related biotechnological innovation, such as identification of suitable methanotrophic strains with better and/or novel metabolic activity, development of high-cell density mass cultivation technology, and the microbial consortium (methanotrophs and non-methanotrophs consortium) design and control technology.

선형조인트 내화충전구조 시스템 내화물(암면)의 압축 및 보온재 여부에 따라 내화성능에 미치는 영향에 관한 실험적 연구

조경숙(Cho, Kyung-Suk),여인환(Yeo, In-Hwan),전기수(Jeon, Ki-Soo),최동호(Choi, Dong-Ho) 대한건축학회 2013 대한건축학회 학술발표대회 논문집 Vol.33 No.2

도심 학교 토양의 메탄 산화 및 생성 잠재력 평가

조경숙 ( Kyung Suk Cho ),류희욱 ( Hee Wook Ryu ),김태관 ( Tae Gwan Kim ),이윤영 ( Yun Yeong Lee ) 한국미생물생명공학회 2014 한국미생물·생명공학회지 Vol.42 No.1

Methane oxidation and the production potentials of ground soil (soil A) and garden soil (soil B, C, & D) in an urban school were evaluated, and the methanotrophic and methanogen communities in the soil samples were quantified using quantitative realtime PCR. The methanotrophic community in the raw soil A sample possessed a 6.1×103 gene copy number/g dry weight soil, whereas those in the raw soils B~D samples were 1.6-1.9 × 105 gene copy numbers/g dry weight soil. Serum bottles added with the soil samples were enriched with methane gas, and then evaluated for their methane oxidation potential. The soil A sample had a longer induction phase for methane oxidation than the other soils. However, soil A showed a similar methane oxidation potential with soils B~D after the induction phase. The methanotrophic community in the enriched soil A sample was increased by up to 2.3 × 107 gene copy numbers/g dry weight soil, which had no significantly difference compared with those in soils B~D (1.2-2.8 × 108 gene copy numbers/g dry weight soil). Methane production showed a similar tendency to methane oxidation. The methanogens community in raw soil A (1.7 × 105 gene copy number/g dry weight soil) was much less than those in raw soils B~D (1.3-3.4 × 107 gene copy numbers/g dry weight soil). However, after methane gas was produced by adding starch to the soils, soil samples A~D showed 107 gene copy numbers/g dry weight soil in methanogens communities. The results indicate that methanotrophic and methanogenic bacteria have coexisted in this urban school`s soils. Moreover, under appropriate conditions for methane oxidation and production, methanotrophic bacteria and methanogens are increased and they have the potential for methane oxidation and production.

성능기반설계도입을 위한 실물스케일 화재안전 성능평가 사례 연구

조경숙(Cho Kyung-Suk),김흥열(Kim Heung-Youl),김형준(Kim Hyung-Jun) 대한건축학회 2010 대한건축학회 학술발표대회 논문집 Vol.30 No.1