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Isolation and Purification of Protoplasts from PorPhyra tenera Thalli
송상호,정규화,SONG Sang Ho,CHUNG Gyu Hwa 한국양식학회 1988 韓國養殖學會誌 Vol. No.
김 (Porphyra tenera)의 생물공학적인 육종법개발을 위해 그의 엽체를 효소처리하여 원형질체를 분리하고 정제하였다. 원형질체의 수율은 효소의 농도와 효소액에 의한 조직의 처리시간에 비례하였다. $12\%$의 전복 내장 건조 분말로 조제된 효소액으로 $22^{\circ}C$에서 3시간동안 처리했을때 약 $2.5\times10^6$ 개의 원형질체가 분리되었다. 원형질체들의 크기는 $9\~25\mu$m로 다양했으며 평균 14.5$\mu$m였다. Protoplasts were isolated enzymatically from thalli of Porphyra tenera in relation to its utilization in breeding. As the concentration of the enzymes and incubation period increased, so did the yield of protoplasts. When a 250 mg fresh weight of the thalli was incubated in the $12\%$ abalone digestive enzyme mixture at $22^{\circ}C$ for 3 hours, about $2.5\times10^6$ protoplasts were released. The size of protoplasts ranged between 9$\mu$m and 25$\mu$m, with an average of 14.5$\mu$m.
안창범(Chang Bum AHN),정규화(Gyu Hwa CHUNG) 전남대학교 수산과학연구소 1995 수산과학연구소논문집 Vol.4 No.-
To obtain basic data about crustacean shell, chitin and amino acid content of six species of crustacean shells(Antarctic krill, Euphausia superba; Red snow crab, Chinonecetes japonicus, Daelongsuyum shrimp, Solenocera prominetis, Lobster, Linuparus trigonus; Gasibal shrimp, Nephrops thomsoni; Blue crab, Portunus trituberculatus) were investigated. The chitin content of six species of crustacean shells extracted by Hackman method ranged from 11.2 to 22.7% and that of the leg shell of red snow crab was the highest of them, holding 22.7%. But the chitin contained a small amount of protein, in the range of 0.5 to 3.1%. Total amino acid content of the six species of decalcified shells ranged from 55856.1 mg/100g to 30807.6 mg/100g (55856.1 mg/100g in antarctic krill shell, 37983.4 mg/100g in red snow crab shell, 41059.3 mg/100g in daelongsuyum shrimp shell, 36275.2 mg/100g in lobster shell, 33114.9 mg/100g in gasibal shrimp shell, and 30807.6 mg/100g in blue crab shell). The great portion of amino acids in these decalcified shells was occupied by aspartic acid, glutamic acid, and histidine. In the case of decalcified shell of gasibal shrimp, histidine was responsible for 59.8% of the total amino acid.