Association between Genetic Variants in Pre-microRNAs and Survival of Early Stage Non-small Cell Lung Cancer

이용훈,( Mi Jeong Hong ),( Shin Yup Lee ),( Seung Soo Yoo ),( Jae Hee Lee ),( Seung Ick Cha ),( Chang Ho Kim ),( Eung Bae Lee ),( Hyo Sung Jeon ),( Ji Woong Son ),( Jae Yong Park ) 대한결핵 및 호흡기학회 2012 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.114 No.-

Introduction: Altered miRNA expression plays an important role in the initiation and progression of lung cancer. Single nucleotide polymorphisms (SNPs) in pre-miRNAs could alter miRNAs processing, or expression, and hence, could influence the prognosis of lung cancer. We evaluated the effects of four SNPs in pre-miRNAs (pre-miR-146a rs2910164, pre-miR-149 rs2292832, pre-miR-196a rs11614913, and pre-miR-499 rs3746444) on the survival outcomes in early-stage NSCLC. Methods: Surgically resected 363 NSCLC patients were enrolled. The four SNPs were genotyped using a PCR-RFLP assay. The genotype associations with overall survival (OS) and disease free survival (DFS) were analyzed. Results: The pre-miR-149 rs2292832T>C and pre-miR-196a rs11614913C>T were found to be significantly associated with OS and DFS. The rs2292832 TC or CC genotype exhibited a significantly better OS and DFS compared to the rs2292832 TT genotype (adjusted hazard ratio [aHR] for OS=0.66, p=0.01; and aHR for DFS=0.64, p=0.004). For the pre-miR-196a rs11614913C>T, patients with the CT or TT genotype had a significantly better OS and DFS than those with the CC genotype (aHR for OS=0.70, p=0.05; and aHR for DFS=0.66, p=0.01). When the two SNPs were combined, OS and DFS improved in a dose-dependent manner as the number of good genotypes increased (p=0.002; p=0.0001). Conclusions: These results suggest that miR-149 and miR-196a may be involved in the pathogenesis of NSCLC and that rs2292832 and rs11614913 can be used as prognostic markers for patients with surgically-resected early stage NSCLC.

Telomere Length of Tumor Tissues and Survival in Patients with Early Stage Non-Small Cell Lung Cancer

이용훈,( Hyo Sung Jeon ),( Eung Bae Lee ),( Seung Soo Yoo ),( Shin Yup Lee ),( Jaehee Lee ),( Seung Ick Cha ),( Chang Ho Kim ),( Jae Yong Park ) 대한결핵 및 호흡기학회 2012 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.114 No.0

Introduction: Telomere shortening leads to genomic instability that drives oncogenesis through the activation of telomerase and the generation of other mutations necessary for tumor progression. This study was conducted to determine the impact of telomere shortening on the survival of patients with early stage non-small cell lung cancer (NSCLC). Methods: Relative telomere length in tumor tissues was measured by quantitative polymerase chain reaction in 164 patients with surgically-resected NSCLC. The association between telomere length and overall survival (OS) and disease-free survival (DFS) was analyzed. Results: When the patients were categorized into quartiles based on telomere length, those patients with the 1st quartile (shortest) of telomere length had a significantly worse OS and DFS compared to patients with the 2nd to the 4th quartiles of telomere length (adjusted hazard ratio for OS=2.67, 95% confidence interval=1.50-4.75, p=0.001; and adjusted hazard ratio for DFS=1.92, 95% confidence interval=1.17-3.14, p=0.01). An association between telomere length and survival outcome was more pronounced in squamous cell carcinomas than adenocarcinomas (p-value of test for homogeneity for OS and DFS=0.05 and 0.02, respectively). Conclusion: Telomere length of tumor tissues is an independent prognostic factor in patients with surgically resected early stage NSCLC.

Clinical and radiological features of pulmonary tuberculosis in patients with idiopathic pulmonary fibrosis

이용훈 ( Seung-ick Cha ),( Jae-kwang Lim ),( Seung-soo Yoo ),( Shin-yup Lee ),( Jaehee Lee ),( Chang-ho Kim ),( Jae-yong Park ) 대한결핵 및 호흡기학회 2018 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.126 No.-

Background: Data regarding the radiological and clinical features of pulmonary tuberculosis (PTB) in patients with idiopathic pulmonary fibrosis (IPF) are limited. Objectives: To investigate the clinico-radiological features of PTB patients with IPF. Methods: Clinico-radiological variables were retrospectively compared between PTB patients with IPF (TB-IPF) and without IPF (TB-control), and computed tomography (CT) findings between the TB-IPF group and patients with nontuberculous mycobacterial lung disease and IPF (NTM-IPF). Results: Of 609 IPF patients, 28 (4.6%) were diagnosed with PTB. In the TB-IPF group, incidental radiological finding was the most common presenting manifestation, and the rate of treatment success was significantly lower than in the TB-control group. On CT scan, the typical locations of reactivated PTB were significantly less often involved in the TB-IPF group than in the TB-control group. The TB-IPF group exhibited a significantly lower frequency of centrilobular nodules and a higher rate of consolidation-predominant pattern than the TB-control group. CT findings in the TB-IPF group were similar to those in the NTM-IPF group. Conclusions: Incidental radiological finding was the most common presenting manifestation in patients with TB-IPF, who were more likely to present with atypical PTB manifestations on CT scan and experience a poorer treatment outcome.

지역사회획득 메티실린 내성 황색포도구균 폐렴 1예

이용훈 ( Yong Hoon Lee ),차승익 ( Seung Ick Cha ),이소연 ( So Yeon Lee ),최선하 ( Sun Ha Choi ),오세림 ( Se Rim Oh ),서혜원 ( Hye Won Seo ),김정민 ( Jung Min Kim ) 대한내과학회 2013 대한내과학회지 Vol.84 No.5

입원력이나 뚜렷한 병원접촉이 없던 환자에서 발생하는 지역사회획득 메티실린 내성 황색포도구균(CA-MRSA) 감염의 중요성이 최근 대두되고 있다. CA-MRSA는 병원획득 MRSA와 다른 유전형과 항생제 감수성을 보인다. CA-MRSA는 주로 피부 및 연조직 감염의 형태로 나타나지만 점차 폐렴과 같은 중증 감염의 사례도 증가하고 있다. 그러나 국내에서 CA-MRSA 폐렴은 아직 매우 드문 실정이다. 이에 저자들은 최근 유전형을 확인한 CA-MRSA 폐렴 1예를 경험하였기에 문헌고찰과 더불어 보고하는 바이다. Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) has received considerable attention in recent years as the cause of infections in individuals in the community who do not have traditional risk factors for MRSA infection, such as hospitalization or contact with healthcare services. CA-MRSA strains have different molecular and antimicrobial susceptibilities, as compared to hospital-associated MRSA. Although CA-MRSA strains are primarily associated with skin and soft tissue infections, they can cause more invasive infections, such as severe community-acquired pneumonia. Reports on CA-MRSA pneumonia in Korea are sparse. Therefore, we report a case of CA-MRSA pneumonia with molecular typing of the MRSA isolate. (Korean J Med 2013;84:728-732)

활동성 결핵의 중증도 및 병변 부위에 따른 전혈 인터페론 감마 분비능 측정의 민감도

김이영 ( Yi Young Kim ),이재희 ( Jae Hee Lee ),이윤지 ( Yoon Jee Lee ),이소연 ( So Yeon Lee ),이용훈 ( Yong Hun Lee ),최금주 ( Keum Ju Choi ),황보엽 ( Bo Yup Hwang ),차승익 ( Seung Ick Cha ),박재용 ( Jae Yong Park ),정태훈 ( Tae H 대한결핵 및 호흡기학회 2011 Tuberculosis and Respiratory Diseases Vol.70 No.2

Background: The clinical manifestation of M. tuberculosis infection ranges from asymptomatic latent infection, to focal forms with minimal symptoms and low bacterial burdens, and finally to advanced tuberculosis (TB) with severe symptoms and high bacillary loads. We investigated the diagnostic sensitivity of the whole-blood interferon-y release assay according to the wide spectrum of clinical phenotypes. Methods: In patients diagnosed with active TB that underwent QuantiFERON(R) (QFT) testing, the QFT results were compared with patients known to be infected with pulmonary tuberculosis (P-TB) and extra-pulmonary TB (EP-TB). In addition, the results of the QFT test were further analyzed according to the radiographic extent of disease in patients with P-TB and the location of disease in patients with EP-TB. Results: There were no statistical differences in the overall distribution of QFT results between 177 patients with P-TB and 84 patients with EP-TB; the positive results of QFT test in patients with P-TB and EP-TB were 70.1% and 64.3%, respectively. Among patients with P-TB, patients with mild extents of disease showed higher frequency of positive results of QFT test than that of patients with severe form (75.2% vs. 57.1%, respectively; p=0.043) mainly due to an increase of indeterminate results in severe P-TB. Patients with TB pleurisy showed lower sensitivity by the QFT test than those with tuberculous lymphadenitis (48.8% vs. 78.8%, respectively; p=0.019). Conclusion: Although QFT test showed similar results between overall patients with P-TB and EP-TB, individual sensitivity was different according to the radiographic extent of disease in P-TB and the location of disease in EP-TB.