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가금의 인 및 칼슘 대사에 관한 연구 ; 제5보 : 초생추 각장기에 있어서 이중투여에 의한 P32 및 Ca45 분포에 관한 실험적 연구
이용빈 ( Yong Bin Lee ),정영채 ( Yang Chai Chung ),김영묵 ( Yong Mook Kim ),성재기 ( Jae Ki Sung ),권종국 ( Jong Kuk Kwun ) 한국축산학회 1967 한국축산학회지 Vol.9 No.1
The distribution of double labeled P^(32) and Ca^(45) in the various tissues were investigated in fourty chicken. The results obtained were as follows: 1) The highest differences were observed in the thyroid gland between the sex in 24 hrs. after double administration of P^(32) and Ca^(45). 2) Generally, the distribution of double administrated P^(32) and Ca^(45) were higher in 24 hours than in one hour. 3) The sequence of the distribution of P^(32) and Ca^(45) in Various tissue were, thyroid gland, femur, adrenal, genital organs, pituitary, kidney, liver, spleen, brain, and G-muscle.
초생추 각장기에 있어서 이중투여에 의한 P32 및 Ca45 분포에 관한 실험적 연구
이용빈 ( Yong Bin Lee ),정영채 ( Yang Chai Chung ),김영묵 ( Yong Mook Kim ),성재기 ( Jae Ki Sung ),권종국 ( Jong Kuk Kwun ) 한국축산학회 1967 한국축산학회지 Vol.9 No.1
The distribution of double labeled P^(32) and Ca^(45) in the various tissues were investigated in fourty chicken. The results obtained were as follows: 1) The highest differences were observed in the thyroid gland between the sex in 24 hrs. after double administration of P^(32) and Ca^(45). 2) Generally, the distribution of double administrated P^(32) and Ca^(45) were higher in 24 hours than in one hour. 3) The sequence of the distribution of P^(32) and Ca^(45) in Various tissue were, thyroid gland, femur, adrenal, genital organs, pituitary, kidney, liver, spleen, brain, and G-muscle.
생쥐에 있어서 투명대의 용해성과 분리할구의 발생 가능성에 관한 연구
양보석,임경순,이용빈 ( B . S . Yang,K . S . Im,Y . B . Lee ) 한국축산학회 1985 한국축산학회지 Vol.27 No.11
The present study was carried out to investigate the cloning of mouse embroys. Female ICR micx were superovulated by intraperitonial injection of PMSG and HCG. Two- and four-cell embryos were digested by proteolytic enzymes; pronase, trypsin, protease and pepsin, and lipase, and separated in vitro by fine glass needles with hand and isolated blastomeres were cultured in vitro for 72 hours. The results obtained were summarized as follow; 1. The proteolytic enzymes had been shown to cause dissolution of zona pellucida, but lipase didn`t. 2. In two-cell mouse embryos, the rate of dichotomy was 89.7% and 97.6 k in zona softened and removed, respectively. Among the dichotomized two-cell mouse embryos, one half and both halves undamaged blastomeres were 30.8% and 57.9% in zone softened and 12.2% and 85.4% in zona removed, respectively. 3. In four-cell mouse embryos, the rate of dichotomy was 85.3% and 96.0% in zona softened and removed. The rate of 4:0, 3:1, 2:2 and 1:3 (undamaged blastomeres vs. damaged blastomeres) were 8.8%, 15.7%, 42.2% and 18.6% in zona softened and 16.8%, 30.9%, 35.6% and 12.8% in zona removed, respectively. 4. The developmental rate of zona softened and removed single blastomeres was 68.5% and 69.0% in 2cell embryo, and 46.7% and 38.5% in 4-cell embryo, respectively. And the developmental rate of zona softened and removed single blastomeres was 10.5% and 6.9% in 2~ell embryo, and 0% and 0% in 4-cell embryo, respectively. The blastomeres isolated from 2-cell embryo developed into blastocyst, but that from 4-cell embryo developed into 8-cell stage, respectively.