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Seedcoat removal and seed germination in Helianthus tuberosus L.
Lim, K.B.,Lee, H.J.,Lee, S.R.,Lee, J.I.,Ahn, S.D. 순천대학교 기초과학연구소 1990 基礎科學硏究誌 Vol.1 No.-
植物學的 種子 (botanical seed)에 의한 돼지감자의 實生繁殖을 시도하였다. J-42等 5種의 돼지감자 花器에서 種子를 채취하여 室內에 보관하여 오면서 發芽를 誘導하였으나 저장期間의 4個月 되는 時期까지 發芽시킬 수 없었다. 그러나 種子의 果皮(pericarp)와 種皮(seedcoat)를 모두 除去하는 處理에서 完全한 發芽誘導가 可能하였는데 特히 種子의 果皮除去만으로는 發芽誘導效果를 볼 수 없었다. 이러한 結果로부터 돼지 감자의 植物學的 種子의 경우 種子의 種皮가 種子의 不發芽에 깊이 관련되어 있음을 확인 할 수 있었다. 또한 돼지감자 種子의 種皮除去에 의한發芽誘導方法은 수집種에 따른 차이 없이 어느 種에서나 發芽誘導에 매우 效果的이었다. Pericarp and seedcoat removal treatments were tested to determine their effectiveness in the hard-to-germinate botanical seeds of Jerusalem artichoke. Fresh seed of five Helianthus tuberosus L. varieties were (A) untreated (B) water soaked overnight (C) soaked overnight followed by removal of pericarp or (D) soaked overnight followed by removal of pericarp and seedcoat. The results indicate that treatments which removed the pericarp and seedcoat were the very effective, giving germination of over 90%. A considerable increase in germination did not follow only the pericarp removal treatments. The factors inhibitory to germination of fresh Jerusalem artichoke seed is associated with the seedcoat. The removal treatment of pericarp and seedcost id recommended despite its complexity because it gives a high germination percentage and varies least from variety to variety. Helianthus tuberosus L., a biomass potential crop, is a member of the family compositae. The genus Helianthus has provided man with two food plants, the sunflower (H. annus)and the jersalem artichoke or topinambour (H. tuberosus). (3) The jersalem artichoke grown for its tubers, has always been an extremely minor crop, but it is still grown in many places as a food for man or livestock and propagation Jerusalem artichoke also flower and pruduce seed in head-like in florescences. Folwers are developed acropetally on flattened receptacles such that outermost flowers deffernt in genotype from others. But the variable response of varieties to the pericarp and seedcoat removal treatment lead us to this treatment as the best method for breaking hard-to-germinate trait in the botanical seed of H. tuberosus. Results obtained suggest that the factors inhibitory to germination is located in the seedcoat. These data also indicate inhibition may be physical rather than chemical since increased seed germination observed following pericarp removal could probably be attributer to damaged seedcoats, even thogh seedcoats appeared to be intact. However, it should be noted that this experiment was not designed to exclude chemical inhibitors as possible retardant to germination of freshly harvested seeds.