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함준수(Joon Soo Hahm),박경근(Kyung Geun Park),박준용(Joon Yong Park),한동수(Dong Soo Han),이민호(Min Ho Lee),기춘석(Choon Suhk Kee),박경남(Kyung Nam Park),이광수(Kwang Soo Lee),최은아(Eun A Choi),이명규(Myung Kyo Lee),함경수(Kyung So 대한소화기학회 1996 대한소화기학회지 Vol.28 No.1
N/A Background/Aims: Biliary proteins have been suggested to play an important role in nucleation and gallstone formation. However, the exact roles and characteristics have not been completely documented. The aim of the present study is to isolate and characterize the nucleating protein extracted from gallstones. Methods: We tried to extract, isolate and characterize proteins in patients with gallstones. Twenty-two gallstones were obtained(12 cholesterol, 10 pigment) at cholecystec- tomy and extracted with ethanol/ether mixture. Then, isoelectric focusing was performed and gallstone proteins were analysed by SDS-polyacrylamide gel electrophoresis. The amino acids were also analysis by the autoamimo acid analyzer system. Results: The mean amount of gallstone protein was 4.15 mg/(g stone) in cholesterol and 16.15 mg/(g stone) in pigment stone. The proteins from both cholesterol and pigment stones showed major bands at low pH on isoelectric focusing. On SDS-PAGE, low molecular protein bands were noted, mainly below 45KD in both cholesterol and pigment stones. The composition of aspartate and glutamate was 21.6% in cholesterol stones and 22.7% in pigment stones. Conclusions: The proteins in cholesterol and pigment gallstones are low molecular weight acidic proteins, and these acidic proteins seem to play an important role in the pathogenesis of gall stones. However, it remains to be determined whether these proteins differ in functional roles from different gallstones. (Korean J Gastroenterol 1996;28: 92 - 100)