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        Microdevice for Separation of Circulating Tumor Cells Using Embedded Magnetophoresis with V-shaped Ni-Co Nanowires and Immuno-nanomagnetic Beads

        박정원,이내림,조성목,정문윤,임춘화,이대식 한국전자통신연구원 2015 ETRI Journal Vol.37 No.2

        The novelty of this study resides in a 6”-wafer-level microfabrication protocol for a microdevice with a fluidic control system for the separation of circulating tumor cells (CTCs) from human whole blood cells. The microdevice utilizes a lateral magnetophoresis method based on immunomagnetic nanobeads with anti-epithelial cell adhesive molecule antibodies that selectively bind to epithelial cancer cells. The device consists of a top polydimethylsiloxane substrate for microfluidic control and a bottom substrate for lateral magnetophoretic force generation with embedded v-shaped soft magnetic microwires. The microdevice can isolate about 93% of the spiked cancer cells (MCF-7, a breast cancer cell line) at a flow rate of 40/100 mL/min with respect to a whole human blood/buffer solution. For all isolation, it takes only 10 min to process 400 mL of whole human blood. The fabrication method is sufficiently simple and easy, allowing the microdevice to be a mass-producible clinical tool for cancer diagnosis, prognosis, and personalized medicine.

      • Investigation of Microchannel Wettability on the Formation of Droplets and Efficient Mixing in Microfluidic Devices

        최창형,Naveen Prasad,이내림,이창수 한국바이오칩학회 2008 BioChip Journal Vol.2 No.1

        This study is an experimental investigation of a microfluidic method for the formation of droplets and mixing efficiency in continuous microfluidic channels. Droplet size strongly depends on the total aqueous flow rate under a fixed oil flow rate. In the case of different wetting properties, a hydrophobically homogeneous microfluidic channel produces smaller droplets than that of a heterogeneous microfluidic channel b ecause of the different wettability of the formed aqueous droplets. However, high mixing efficiency in the two types of microfluidic channel is achieved by droplet recirculation. Although an increase in droplet size demands a longer mixing time, the microfluidic approach provides rapid mixing and no reagent dispersion. This mixing method in microfluidics can be applied to a variety of chemical syntheses or biochemical reactions at the nanoliter scale. This study is an experimental investigation of a microfluidic method for the formation of droplets and mixing efficiency in continuous microfluidic channels. Droplet size strongly depends on the total aqueous flow rate under a fixed oil flow rate. In the case of different wetting properties, a hydrophobically homogeneous microfluidic channel produces smaller droplets than that of a heterogeneous microfluidic channel b ecause of the different wettability of the formed aqueous droplets. However, high mixing efficiency in the two types of microfluidic channel is achieved by droplet recirculation. Although an increase in droplet size demands a longer mixing time, the microfluidic approach provides rapid mixing and no reagent dispersion. This mixing method in microfluidics can be applied to a variety of chemical syntheses or biochemical reactions at the nanoliter scale.

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