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경남 한산·거제만해역에서 생산된 패류의 위생학적 안전성 평가
하광수,이가정,정연중,목종수,김풍호,김연계,이희정,김동욱,손광태,Ha, Kwang-Soo,Lee, Ka-Jeong,Jeong, Yeon-Jung,Mok, Jong-Soo,Kim, Poong-Ho,Kim, Yeon-Kye,Lee, Hee-Jung,Kim, Dong-Wook,Son, Kwang-Tae 한국식품위생안전성학회 2018 한국식품위생안전성학회지 Vol.33 No.5
본 연구는 2013년부터 2017년까지 경남 한산 거제만 해역에서 생산되는 패류에 대한 위생지표세균, 마비성 패류독소, 설사성 패류독소를 분석하여 세균학적 독물학적 안전성을 평가하였다. 굴 시료 404점에 대한 분변계대장균은 < 18~330 MPN/100 g의 범위를 나타내었으나, 대장균 230 E. coli MPN/100 g을 초과한 시료는 없었다. 굴 수확시기 동안 분석한 대장균의 기하학적 평균치는 24.3 MPN/100 g으로 매우 낮게 나타났다. 한산 거제만해역에서 생산되는 굴의 세균학적 안전성을 평가한 결과, 식품위생법의 생식용 굴에 대한 위생기준을 만족하였고, EU의 패류생산해역 A등급 기준에 부합하였다. 한산 거제만해역의 독물학적 평가를 위해 굴 532개 시료와 패류독소 지표종인 지중해담치 268개 시료에 대한 분석을 실시하였다. 마비성패류독소는 2013년 4월에 지중해담치 3개 시료에서 1.20~2.29 mg/kg 범위로 기준을 초과하여 검출되었으나, 굴시료에서는 전혀 검출되지 않았다. 총 120개 시료에서 설사성패류독소를 분석하였으나, 정량한계 이하로 매우 낮게 나타났다. 독물학적 안전성 평가결과, 굴 시료에서는 마비성 및 설사성패류독소가 전혀 검출되지 않았으나, 지표종인 지중해담치에서는 마비성패류독소 기준치를 초과한 것으로 나타나 지속적인 모니터링과 안전관리가 요구되었다. To evaluate bacteriological and toxicological safety hygienic indicator bacterium and paralytic and diarrhetic shellfish toxins in the shellfish produced in Hansan Geojeman 2013-2017 were investigated. Fecal coliforms were < 18~330 MPN/100 g in 404 oyster samples. But all samples tested, did not exceed 230 E. coli MPN/100 g. Geometric mean of E. coli for oyster samples collected during major shellfish production period was 24.3 MPN/100 g, considerde stable results. Bacteriological quality of oysters collected from Hansan Geojeman meets the standard value based on shellfish hygiene of the Food Sanitation Act of Korea and also meets Grade A, according to classification of shellfish harvesting areas of the European Union. For toxicological evaluation of Hansan Geojeman, 532 oyster samples and 268 mussel samples as an indicator, were analyzed. Paralytic shellfish toxins were detected in the range of 0.42~2.29 mg/kg in eight mussel samples, and exceeded criteria in three samples from early to late April 2013. Diarrhetic shellfish toxin was detected in three of 120 samples, but it was revealed to be under regulation value (0.16 mg Okadaic Acid equ./kg). As a result of toxicological evaluation, paralytic and diarrhetic shellfish toxins were not detected in oyster samples, but it was found that mussel as an indicator species, exceeded the threshold value of paralytic shellfish toxin. Accordingly, sanitary surveys were continuously requested for food safety management of shellfish.
창선 해역의 지중해담치(Mytilus galloprovincialis) 양식장 및 육상오염원에서 분리한 대장균(Escherichia coli)의 항생제 내성
권순재 ( Soon Jae Kwon ),이가정 ( Ka Jeong Lee ),정연중 ( Yeoun Joong Jung ),박상기 ( Sang Gi Park ),고경리 ( Kyeong Ri Go ),양지혜 ( Ji Hye Yang ),목종수 ( Jong Soo Mok ) 한국수산과학회(구 한국수산학회) 2016 한국수산과학회지 Vol.49 No.5
In this study, we isolated and characterized Escherichia coli from mussels and inland pollution sources in or in proximity to the Changseon area on the southern coast of Korea in 2014. A total of 147 strains of E. coli were isolated from 54 mussels and 32 pollution-source samples. The susceptibility of the isolates to 24 antimicrobial agents was analyzed. The resistance of E. coli isolates to rifampin was highest at 100%, followed by cephalothin (98.6%), tetracycline (91.8%), amikacin (81.0%), ampicillin (79.6%), cefazolin (79.6%), streptomycin (73.5%), piperacillin (70.7%), gentamicin (37.4%), cefoxitin (35.4%), cefamandole (34.7%), tobramycin (29.9%), amoxicillin/clavulanic acid (24.5%), nalidixic acid (21.8%), trimethoprim (19.0%), chloramphenicol (17.7%), cefotaxime (12.9%), trime-thoprim (10.9%), ceftazidime (10.2%), aztreonam (7.5%), imipenem (2.7%), cefepime (2.0%), and cefotetan (0.0%). In addition, the antimicrobial resistance of E. coli isolates from inland pollution sources was slightly greater than or similar to that of isolates from mussels.
2014-2016년 경남 용남·광도해역 해수 및 굴(Crassostrea gigas)의 세균학적 위생평가
윤현진 ( Hyun Jin Yoon ),권지영 ( Ji Young Kwon ),이가정 ( Ka Jeong Lee ),권순재 ( Soon Jae Kwon ),목종수 ( Jong Soo Mok ),김풍호 ( Poong Ho Kim ),정연중 ( Yeoun Joong Jung ) 한국수산과학회(구 한국수산학회) 2018 한국수산과학회지 Vol.51 No.5
This study evaluated the sanitary state of seawater and shellfish in Yongnam-Gwangdo area from January 2014 to December 2016. The sampling stations for sanitary survey in Yongnam-Gwangdo area were composed of 41 seawater stations and 5 oyster Crassostrea gigas stations. The samples were collected monthly at each station. Shellfish-borne disease is associated with bacteria and viruses in the presence of fecal coliforms. Bacteriological pollution levels of shellfish increase with seawater quality. Therefore, fecal coliforms are very important criteria for evaluating the safety of fisheries in coastal areas. The geometric mean and the estimated 90th percentile ranges of total and fecal coliforms for seawater were 1.8-11.7, 2.8-233.6, <1.8-6.7 and 1.8-100.2 MPN/100 mL, respectively. The range of E. coli levels for oysters was <20-1,300 MPN/100 g. Based on various standards, the sanitary condition in Yongnam- Gwangdo area was evaluated as follows: clean area (Korean criteria), conditionally approved area (US criteria) and class B (EU criteria).
산 처리에 의한 김(Pyropia sp.)의 유해 중금속(Cd, Cr, Pb) 제거 효과
목종수 ( Jong Soo Mok ),손광태 ( Kwang Tae Son ),이태식 ( Tae Seek Lee ),이가정 ( Ka Jeong Lee ),정연중 ( Yeoun Joong Jung ),김지회 ( Ji Hoe Kim ) 한국수산과학회(구 한국수산학회) 2016 한국수산과학회지 Vol.49 No.5
We examined the removal of hazardous heavy metals (Cd, Cr, and Pb) from laver Pyropia sp. using citric, hydrochloric, and nitric acids. Under the same conditions, the quality of the laver samples was also evaluated using the variation in absorbance and major mineral levels. The heavy metals that accumulated in raw laver samples after 3 days in seawater included Pb (117.79 μg/g), Cr (33.53 μg/g), and Cd (10.54 μg/g) in descending order. The rate of heavy metal removal from laver was higher at lower pH for all acids used. However, its color changed unsatisfactorily at pH 2.0. After 10 min in seawater at pH 2.5, the heavy metals in laver were eliminated in the order Cd (68.7-81.6%), Pb (57.7-67.0%), and Cr (31.9-49.4%) using the three acids. The differences in heavy metal removal among acid types were not significant. The laver quality was not affected after 20 min at the pH range of 2.5-4.0. The maximum removal of heavy metals was from laver soaked for 10 min in seawater at pH 2.5 using the organic acid, citric acid.
불가사리(Asterias amurensis) 추출물을 첨가한 사료의 급이가 조피볼락 (Sebastes schlegeli)의 성장, 혈액성상 및 식세포 활성산소 생산에 미치는 효과
박희연 ( Hee Yeon Park ),임치원 ( Chi Won Lim ),김연계 ( Yeon Kye Kim ),최태진 ( Tae Jin Choi ),윤호동 ( Ho Dong Yoon ),이가정 ( Ka Jung Lee ),서연경 ( Yeon Kyung Seo ),김지영 ( Ji Yeong Kim ),박기의 ( Ki Eui Park ) 한국응용생명화학회 2007 Applied Biological Chemistry (Appl Biol Chem) Vol.50 No.4