족소양담경(足少陽膽經)에서 투사(投射)되는 신경원(神經元)의 표지부위(標識部位)에 대한 연구(硏究)

육상원,이광규,이상룡,김점영,이창현,이봉희,Ryuk Sang-Won,Lee Kwang-Gyu,Lee Sang-Ryoung,Kim Jum-Young,Lee Chang-Hyun,Lee Bong-Hee 경락경혈학회 2000 대한침구의학회지 Vol.17 No.1

The purpose of this morphological studies was to investigate the relation to the meridian, acupoint and nerve. The common locations of the spinal cord and brain projecting to the the gallbladder, GB34 and common peroneal nerve were observed following injection of transsynaptic neurotropic virus, pseudorabies virus(PRV), into the gallbladder, GB34 and common peroneal nerve of the rabbit. After survival times of 96 hours following injection of PRV, the thirty rabbits were perfused, and their spinal cord and brain were frozen sectioned($30{\mu}m$). These sections were stained by PRV immunohistochemical staining method, and observed with light microscope. The results were as follows: 1. In spinal cord, PRV labeled neurons projecting to the gallbladder, GB34 and common peroneal nerve were founded in thoracic, lumbar and sacral spinal segments. Densely labeled areas of each spinal cord segment were founded in lamina V, VII, X, intermediolateral nucleus and dorsal nucleus. 2. In medulla oblongata, The PRV labeled neurons projecting to the gallbladder, GB34 and common peroneal nerve were founded in the A1 noradrenalin cells/C1 adrenalin cells/caudoventrolateral reticular nucleus, rostroventrolateral reticular nucleus, medullary reticular nucleus, dorsal motor nucleus of vagus nerve, nucleus tractus solitarius, raphe obscurus nucleus, raphe pallidus nucleus, raphe magnus nucleus, gigantocellular nucleus, lateral paragigantocellular nucleus, principal sensory trigeminal nucleus and spinal trigeminal nucleus. 3. In Pons, PRV labeled neurons were parabrachial nucleus, Kolliker-Fuse nucleus and cochlear nucleus. 4. In midbrain, PRV labeled neurons were founded in central gray matter and substantia nigra. 5. In diencephalon, PRV labeled neurons were founded in lateral hypothalamic nucleus, suprachiasmatic nucleus and paraventricular hypothalamic nucleus. 6. In cerebral cortex, PRV labeled neuron were founded in hind limb area.This results suggest that PRV labeled common areas of the spinal cord projecting to the gallbladder, GB34 and common peroneal nerve may be first-order neurons related to the somatic sensory, viscero-somatic sensory and symapathetic preganglionic neurons, and PRV labeled common area of the brain may be first, second and third-order neurons response to the movement of smooth muscle in gallbladder and blood vessels.These PRV labeled neurons may be central autonomic center related to the integration and modulation of reflex control linked to the sensory system monitoring the internal environment, including both visceral sensation and various chemical and physical qualities of the bloodstream. The present morphological results provide that gallbladder meridian and acupoint may be related to the central autonomic pathways.

족소양담경(足少陽膽經)에서 투사(投射)되는 신경원(神經元)의 표식부위(標識部位)에 대한 연구(硏究)

육상원 ( Sang Won Ryuk ),이광규 ( Kwang Gyu Lee ),이상룡 ( Sang Ryoung Lee ),김점영 ( Jum Young Kim ),이창현 ( Chang Hyun Lee ),이봉희 ( Bong Hee Lee ) 대한경락경혈학회 2000 Korean Journal of Acupuncture Vol.17 No.1

The purpose of this morphological studies was to investigate the relation to the meridian, acupoint and nerve. The common locations of the spinal cord and brain projecting to the the gallbladder, GB34 and common peroneal nerve were observed following injection of transsynaptic neurotropic virus, pseudorabies virus(PRV), into the gallbladder, GB34 and common peroneal nerve of the rabbit. After survival times of 96 hours following injection of PRV, the thirty rabbits were perfused, and their spinal cord and brain were frozen sectioned(30㎛). These sections were stained by PRV immunohistochemical staining method, and observed with light microscope The results were as follows: 1. In spinal cord, PRV labeled neurons projecting to the gallbladder, GB34 and common peroneal nerve were founded in thoracic, lumbar and sacral spinal segments. Densely labeled areas of each spinal cord segment were founded in lamina V, VII, X, intermediolateral nucleus and dorsal nucleus. 2. In medulla oblongata, The PRV labeled neurons projecting to the gallbladder, GB34 and common peroneal nerve were founded in the A1 noradrenalin cells/C1 adrenalin cells/caudoventrolateral reticular nucleus, rostroventrolateral reticular nucleus, medullary reticular nucleus, dorsal motor nucleus of vagus nerve, nucleus tractus solitarius, raphe obscurus nucleus, raphe pallidus nucleus, raphe magnus nucleus, gigantocellular nucleus, lateral paragigantocellular nucleus, principal sensory trigeminal nucleus and spinal trigeminal nucleus. 3. In Pons, PRV labeled neurons were parabrachial nucleus, Kolliker-Fuse nucleus and cochlear nucleus. 4. In midbrain, PRV labeled neurons were founded in central gray matter and substantia nigra. 5. In diencephalon, PRV labeled neurons were founded in lateral hypothalamic nucleus, suprachiasmatic nucleus and paraventricular hypothalamic nucleus. 6. In cerebral cortex, PRV labeled neuron were founded in hind limb area. This results suggest that PRV labeled common areas of the spinal cord projecting to the gallbladder, GB34 and common peroneal nerve may be first-order neurons related to the somatic sensory, viscero-somatic sensory and symapathetic preganglionic neurons, and PRV labeled common area of the brain may be first, second and third-order neurons response to the movement of smooth muscle in gallbladder and blood vessels. These PRV labeled neurons may be central autonomic center related to the integration and modulation of reflex control linked to the sensory system monitoring the internal environment, including both visceral sensation and various chemical and physical qualities of the bloodstream. The present morphological results provide that gallbladder meridian and acupoint may be related to the central autonomic pathways.

두부(頭部)의 자기장(磁氣場) 자극(刺戟)이 백서(白鼠)의 하지부(下肢部) 비복근(비腹筋)에 미치는 영향(影響)

장윤성 ( Yoon Sung Jang ),이태영 ( Tae Young Lee ),이광규 ( Kwang Gyu Lee ),이창현 ( Chang Hyun Lee ),육상원 ( Sang Won Ryuk ),이상룡 ( Sang Ryong Lee ) 대한경락경혈학회 2000 Korean Journal of Acupuncture Vol.17 No.2

The purpose of this study was, via analysis of EMG signals, to determine that the motor area of the scalp acupuncture areas is correlated with regulations of motor activity of multiple muscle in the lower limbs. Experimental groups were divided into Normal, Exp I(ipsilateral stimulation group) and Exp II(contralateral stimulation group) groups. The effects on gastrocnemius muscle of hind limb in anaesthetised rats after magnetic stimulation of the motor area of the scalp acupuncture areas were as follows: 1. The mean amplitude in Exp II group was significantly more increased than in Normal group after 30 minutes. (P<0.001) 2. The RMS(root mean square) in Exp II group was significantly more increased than in Normal group after 30 minutes. (P<0.005) 3. The magnetic stimulation of the motor area of the scalp acupuncture areas seems to be correlated with muscle contractibility in the contralateral lower limb. 4. After 30 minutes, the magnetic stimulation to the motor area had an significant effect on the contralateral lower limb.

玄蔘淸肺飮이 항염 및 항알러지 작용에 관한 연구

서정민(Jung Min Seo),김진수(Jin Su Kim),이광규(Kwang Gyu Lee),육상원(Sang Won Ryuk) 한의병리학회 2002 동의생리병리학회지 Vol.16 No.1

口瘡의 문헌연구

정한솔(Han Sol Jung),박종훈(Jong Hoon Park),육상원(Sang Won Ryuk),이광규(Kwang Gyu Lee) 한의병리학회 2002 동의생리병리학회지 Vol.16 No.1

실험적 관절염 흰쥐 모델에서 고삼추출액이 척수와 척수신경절의 CGRP 면역반응 신경원에 미치는 영향

신현종(Hyun Jong Shin),이광규(Kwang Gyu Lee),육상원(Sang Won Ryuk),이상룡(Sang Ryong Lee),고병문(Byung Moon Ko),이창현(Chang Hyun Lee) 한의병리학회 2002 동의생리병리학회지 Vol.16 No.1

淫羊藿 水抽出物이 생쥐의 면역글로불린 및 Cytokine 생성에 미치는 영향

최용덕,이창현,이상룡,육상원,김정훈,우원홍,이광규 대한동의병리학회 2001 동의생리병리학회지 Vol.15 No.2

This present study was carried out to investigate the body weight-regulatory effects of several herbs(Plantaginis Semen; PLS, Zeas Stigma; ZS, Sophorae Radix; SR) in high fat diet-induced obese rats. Control group rats were fed with high fat diet and administered normal saline for 8weeks. Experimental groups rats were fed with high fat diet and administered extract of 3 herbs each other for 8weeks. And observed that, body weight of rats and total cholesterol, triglyceride, free fatty acid, total lipid, phospholipid, high density lipoprotein(HDL)-cholesterol, low density lipoprotein(LDL)-cholesterol in serum of rats, and glucose, insulin of rats. The results were as follows: There were significant decrease of serum total cholesterol level in PLS group and ZS group. There were significant decrease of serum total lipid level in PLS group, ZS group and SR group. There were significant decrease of serum phospholipid level in ZS group. There were significant decrease of serum LDL-cholesterol level in ZS group. There were significant decrease of serum glucose level in PLS group and ZS group. According to above mentioned results, Plantaginis Semen, Zeas Stigma and Sophorae Radix were expected to be applied to the prevention or treatment of obesity and its complications. Key word : Body weight-regulation,Obesity, Plantaginis Semen(車前子), Zeas Stigma(玉米□), Sophorae Radix(苦蔘)

Bufalin이 U937세포의 Apoptosis에 미치는 영향

박민철,정한솔,이광규,이창현,이상룡,육상원 대한동의병리학회 2001 동의생리병리학회지 Vol.15 No.3

Some antitumor reagents including those derived from medicinal herbs exert their action via induction of apoptosis, a process of programmed cell death which, if selective on tumor cells, may have potential value in cancer chemotherapy. Bufalin is a major component of the traditional medicine, Bufobufo gargarizans Cantor which is known to induce typical apoptosis in human leukemia U937 cells. The purpose of this study was to investigate apoptotic change of bufalin treated U937 cell. Method 1 examined U937 cells treated with bufalin by using light microscopy, DNA gel electrophoresis and terminal deoxynucleotidyl transferase-mediated dUTP nick end-labelling(TUNEL) assay to identify apoptosis. Morphological observation and DNA analysis revealed that bufalin caused cell shrinkage with the formation of apoptotic bodies. Cell viability decreased markedly during 12∼24h, Morphological observations that treated with bufalin for 12h, 24h under a light microscope revealed apoptotic change(condensation of chromatin in nuclei and the degradation of nuclei) TUNEL-positive cells were detected in the groups treated for 24h. DNA fragmentation from bufalin-treated cells(for 24h) were observed by agarose gel electrophoresis. These findings indicate that bufalin inhibited the proliferation of U937 cells via induction of apoptosis.