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- 저자 : 유향숙 ( Hyang Sook Yoo ) (검색결과 7 건)
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대장균에서 발현된 효모 Tryptophan Synthetase 의 생화학 및 면역학적 성질에 대한 연구
유향숙,John A . Carbon ( Hyang Sook Yoo,John A . Carbon ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.2
The yeast tryptophan synthetase gene (TRP5) cloned in E. coli is functionally expressed and can complement trpAB deletion mutation in E. coli. We have partially purified the tryptophan synthetase from the yeast, Saccharomyces cerevisiae and trpAB, deleted E. coli containing the yeast recombinant plasmid pYe(trp5)2-11 and examined their biochemical and immunological characteristics. Sephadex G-200 gel filtration of the partially purified enzymes show that the molecular weight of the enzyme produced in E. coli by the pYe(trp5)2-11 is nearly the same (160,000) as that of authentic yeast tryptophan synthetase (155,000). SDS-acrylamide gel analysis of the proteins produced from the E. coli minicells containing yeast TRP5 recombinant plasmid show that the subunit size of yeast tryptophan synthetase produced in E. coli is 72,500, approximately same as the subunit from the native yeast enzyme. Antibody raised against partially purified yeast tryptophan synthetase inactivates the enzyme activity produced in trpAB deleted E. coli by the yeast recombinant plasmids pYe(trp 5). This indicates that tryptophan synthetase produced from the yeast TRP5 gene cloned in E. coli is very similar or identical to the native yeast tryptophan synthetase in its biochemical and immunological characteristics and is different from E. coli tryptophan synthetase. This suggests that large mRNAs must be synthesized from the eucaryotic DNA by the E. coli RNA polymerase and the yeast translational signals are recognized by the E. coli ribosomes, initiation and termination components.
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초등학생들의 정신건강, 학업적 자기효능감, 학교생활적응이 휴대전화 중독에 미치는 영향
유향숙 ( Hyang Sook Yoo ),민경옥 ( Kyoung Ok Min ) 한국아동교육학회 2015 아동교육 Vol.24 No.1
이 연구에서는 초등학생의 정신건강, 학업적 자기효능감, 학교생활적응이 휴대전화 중독 간의 관계를 살펴보았다. 본 연구는 초등학교에 재학중인 5학년과 6학년 349명을 대상으로 SPSS 18.0 프로그램을 이용하여 자료를 분석하였다. 연구결과에 따르면, 성별에서는 내성 및 의존이, 그리고 학년에서는 생활장애가 차이가 있는 것으로 나타났다. 둘째, 상관계수에서는 휴대전화 중독의 하위영역인 강박과 학교생활적응의 하위영역인 담임적응이, 그리고 교우적응은 내성·의존, 생활장애, 강박에서는 유의한 차이가 없는 것으로 나타났다. 셋째, 중다회귀분석에서는 휴대전화 중독에 가장 많은 영향을 미치는 요인은 자기조절효능감, 우울, 적대감, 그리고 규칙 및 학교행사가 영향을 주는 변수로 나타났다. 따라서 이러한 결과에 대해 본 연구의 시사점 및 향후 연구방향에 대해 논의하였다. The purpose of the study is to examine the effect of mental health, academic self efficacy, school life adjustment on cellular phone addiction of elementary. 349 elementary students in Daegu and Seoul province were participated in the survey thru convenient sampling and t-test, conducted by correlation analysis and multiple regression. The results are as follows: First, First, the gender tolerance and dependence, and the grades showed that the difference between the living impaired. Second, the correlation coefficient of the child``s adaptation of compulsion and school adjustment of cell phone addiction, tolerance and friendship adaptation. Dependence, life disability, whereas appeared to be no significant difference in the obsessive, the other variables were found to be significantly correlated. Third, the multiple regression analysis, factors that most influence on cellular phone addiction was found to be self-regulating efficacy, depression, hostility, and the rules and variables that affect school activities. Therefore, we discuss the implications of this study and future research directions for these results.
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Corynebacterium glutamicum의 argEC genes의 분자적 클로닝과 Escherichia coli 내에서의 발현
한영수,유향숙,민경희 숙명여자대학교 자연과학연구소 1993 자연과학논문집 Vol.- No.4
Corynebacterium glutamicum의 DNA를 Sau3AI과 BamHI으로 pBR322를 이용하여 유전자 library를 만든 다음, Escherichia coli의 arg auxotroph에 상보성 실험을 통하여 arginine 합성 유전자인 argEC 유전자를 클로닝하였다. 이 재조합 DNA pARGE2는 4.9 kb로 이 유전자의 제한효소 지도를 Sau3AI, EcoRI, ClaI, BamHI, HindIII, XmnI등을 이용하여 작성하였으며, 재조합 DNA를 deletion analysis를 통하여 argE 유전자의 바로 다음에 argC 유전자가 연결되어 있음을 알 수 있었다. pARGE2를 포함한 형질전환주의 argE의 유전자 산물인 N-acetylglutamate-acetylornithine acetyltransferase (N-acetylornithinase)의 효소활성은 숙주세포인 E. coli JJC16 보다 약 10배나 높게 나타났다. Gene library from Corynebacterium glutamicum chromosomal DNA was constructed with Sau3AI and BamHI using pBR322. argEC gene cluster was cloned by complementation test with E. coli arginine auxotrophs. Recombinant DNA pARGE2 containing 4.9kb was mapped with restriction enzymes. Sau3AI, EcoRI, ClaI, BamHI, HindIII, and XmnI. By deletion analysis of recombinant DNA pARGE2, argE gene was directly linked with argC gene. Transformation containing pARGE2 showed about 10 times higher enzyme activity of N-acetylglutamate-acetylornithine acetyltransferase, argE gene product, compared with that of E. coli JJC16 strain.
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PCR 증폭에 의한 ILPR 신규 Class I Allele 동정
김용성,이대실,유향숙 한국유전학회 1997 Genes & Genomics Vol.19 No.2
The insulin-linked polymorphic region (ILPR) located on the 5' flanking region of the human insulin gene (INS), is considered to be an important transcriptional regulatory region or the susceptibility region of insulin-dependent diabetes mellitus (IDDM). The polymorphism is generated by variation in the number of repeat units within a given ILPR, which determines its overall length, and by minor nucleotide sequence heterogeneity within the individual repeats. Because of high G+C content or highly repetitive sequences in the ILPR, it makes them less amenable to PCR analysis. In this study, we amplified in vitro the ILPR using the PCR mixture containing 40% and 60% 7-deaza-dGTP, a nucleotide analog of dGTP, in stead of the standard PCR mix. The amplified DNA contained an ILPR of 572 bp, indicating a typical class I allele that contains 40 directly repeated oligonucleotides and revealed high G+C content of 72. 4%. The arrangement of the repeat units of the ILPR was different from the six ILPR alleles reported so far. Thus, this DNA sequence can be considered as 7th ILPR allele or 5th class I allele. Therefore, a modified PCR procedure using 7-deaza-dGTP permits to amplify easily or to characterize the class I allele linked to IDDM susceptibility.
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