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금은화의 type I interferon 억제효과 및 기전에 관한 연구
강용구,유익한,김송백,최창민,서윤정,조한백,Kang, Yong-Goo,Ryu, Ik-Han,Kim, Song-Baek,Choi, Chang-Min,Seo, Yun-Jung,Cho, Han-Baek 대한한방부인과학회 2013 大韓韓方婦人科學會誌 Vol.26 No.2
Objectives: The purpose of this study was to investigate whether Lonicera japonica(LJ) could inhibit LPS-induced type I IFN production. Methods: To evaluate inhibitory effect of LJ on type I IFN, we examined type I IFN, IRF-1, 7 and IL-10 production on LPS-induced macrophages using real time RT-PCR. Next, we observed the interaction of type I IFN, IRF-1, 7 and IL-10 using IL-10 neutralizing antibody. Finally we examined the activation of STAT-1, 3 using western blot. Results: LJ inhibited Type I IFN expression of mRNA and increased IL-10 expression of mRNA. Also LJ inhibited the level of IRF-1, 7 mRNA and the nuclear translocation of IRF-3. Further more, LJ reduced the activation of STAT-1, 3 which are involved in continuous secretion of immune cytokines. Blockade of IL-10 action caused a significant reduction of type I IFN and IRF-1, 7 than LPS-induced LJ pretreatment. Conclusions: LJ inhibits LPS-induced production of type I IFN by IL-10. This study may provide a clinical basis for anti-inflammatory properties of LJ.
금은화의 type Ⅰ interferon 억제효과 및 기전에 관한 연구
강용구 ( Yong Goo Kang ),유익한 ( Ik Han Ryu ),김송백 ( Song Baek Kim ),최창민 ( Chang Min Choi ),서윤정 ( Yun Jung Seo ),조한백 ( Han Baek Cho ) 대한한방부인과학회 2013 大韓韓方婦人科學會誌 Vol.26 No.2
Objectives: The purpose of this study was to investigate whether Lonicera japonica(LJ) could inhibit LPS-induced type Ⅰ IFN production. Methods: To evaluate inhibitory effect of LJ on type Ⅰ IFN, we examined type Ⅰ IFN, IRF-1, 7 and IL-10 production on LPS-induced macrophages using real time RT-PCR. Next, we observed the interaction of type Ⅰ IFN, IRF-1, 7 and IL-10 using IL-10 neutralizing antibody. Finally we examined the activation of STAT-1, 3 using western blot. Results: LJ inhibited Type Ⅰ IFN expression of mRNA and increased IL-10 expression of mRNA. Also LJ inhibited the level of IRF-1, 7 mRNA and the nuclear translocation of IRF-3. Further more, LJ reduced the activation of STAT-1, 3 which are involved in continuous secretion of immune cytokines. Blockade of IL-10 action caused a significant reduction of type Ⅰ IFN and IRF-1, 7 than LPS-induced LJ pretreatment. Conclusions: LJ inhibits LPS-induced production of type Ⅰ IFN by IL-10. This study may provide a clinical basis for anti-inflammatory properties of LJ.