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엄기범,김선남,우종표 명지대학교 대학원 1997 대학원논문집 Vol.1 No.-
To prepare monodispersed polymeric hollow particles, seeded multi-stage emulsion polymerization and power-feeding method were used in this study. Methyl methacrylate and methacrylic acid were used as core monomers and styrene as shell monomer with potassium persulfate and sodium dodecylbenzene sulfonate(SDBS) as an initiator and emulsifier, respectively. The morphology of the latex particles was observed by scanning electron microscopy(SEM). It showed that the latices prepared had core/shell structure with hollow. The polymeric hollow particles with size of ca. 550 ∼ 600㎚ and hollow size of ca. 300 ∼ 400㎚ can be manufactured using multi-stage emulsion polymerization.
단량체 투입 방법이 PBA-PMMA 코아/쉘 복합 라텍스 입자의 구조 및 특성에 미치는 영향
엄기범,정재식,권기섭,우종표 명지대학교 대학원 1999 대학원논문집 Vol.3 No.-
Composite latex particles which consist of poly(butyl acrylate) as core polymer and poly(methyl methacrylate) as shell polymer with different morphologies were synthesized by seeded multi-stage emulsion polymerization. Three types of polymers were obtained by various monomer feeling methods: random copolymer, core/shell type copolymer, gradient core shell type copolymer. Particle size was controlled by adjusting the amount of emulsifier. The morphology and particle size were observed by scanning electron microscopy(SEM) and particle analyzer, respectively. Employing these composite latex as an impact modifier of PMMA, mechanical properties including impact strength and tensile strength were examined.
바닐로이드 수용체 TRPV1의 막수송과정에서의 Rab11의 역할
엄기범(Um, Ki-Bum),이순열(Lee, Soon-Youl) 한국산학기술학회 2011 한국산학기술학회논문지 Vol.12 No.7
바닐로이드 수용체 TRPV1(transient receptor potential vanilloid 1)은 캡사이신, pH, 열 등의 통증 유발물질에 의해 활성화되는 비특이적 양이온 채널로서 통증발현에 핵심적인 막 단백질이다. TRPV1의 막 수송에 관한 연구가 미미한 가운데 FIP3(family of Rab11 interacting protein 3)가 TRPV1 채널과 결합하여 막수송에 관여한다고 보고되었 다. FIP3는 Rab11과 결합하는 단백질인데 최근 Rab11 단백질이 여러 채널 단백질의 막수송에 직접적으로 또는 간접 적으로 중요하다고 보고되었다. 그러므로 본 연구에서는 Rab11이 TRPV1의 막 수송에서의 역할을 알아보기 위하여 세포 생물학적, 생화학적으로 알아보았다. 공촛점 현미경을 통하여 확인한 결과 Rab11은 실제로 세포내에서 TRPV1 과 동일한 위치에서 발현되어 있음을 확인하였다. 하지만 생화학적인 방법인 GST-pulldown을 실시하였을 때 TRPV1 과 Rab11간에는 서로 직접적인 결합은 하지 않는 것으로 나타났다. 비록 직접적인 결합은 하지 않지만 Rab11이 TRPV1의 막 수송에 관여한다고 가정하고 Rab11의 TRPV1의 막수송에서의 역할을 더 자세히 알아보기 위하여 세포 내 Rab11a의 발현을 siRNA를 사용하여 Rab11a의 발현을 50%수준으로 저해한 후 TRPV1의 세포막으로의 이동을 알 아본 결과 Rab11 발현 저해 시 세포막에 이동된 TRPV1이 현저히 감소함을 확인할 수 있었다. 이 결과로 부터 Rab11이 아마도 FIP3을 포함하는 방법으로 TRPV1의 막 수송에 영향을 주는 것으로 결론지을 수 있다. Vanilloid receptor, TRPV1 (transient receptor potential vanilloid 1) is a non-selective cation channel that responds to a variety of pain-eliciting material including capsaicin, pH, heat. Although, membrane trafficking of TRPV1 was not much known so far, TRPV1 was reported to interact with FIP3 (family of Rab11 interacting protein 3). FIP3 was identified as one of Rab11 interacting proteins that is recently reported important in membrane trafficking of several channel proteins directly or indirectly. Therefore, in this study, we examined the role of Rab11 in the membrane trafficking of TRPV1 using cell biological and biochemical techniques. Rab11 was found really colocalized with TRPV1 based on the result of confocal microscopy. However, GST-pulldown assay, one of biochemical technique, found that Rab11 did not interact with TRPV1. Although Rab11 does not interact with TRPV1 directly, we hypothesized that Rab11 is indeed involved in the membrane trafficking of TRPV1. In order to examine further the role of Rab11 in the membrane trafficking of TRPV1, the expression of TRPV1 on the membrane was examined when the expression of Rab11 was decreased down to about 50% by siRNA technique and found decreased significantly. From this result, we can conclude that Rab11 is involved in the membrane trafficking of TRPV1 in a way of including FIP3.
다단계 유화중합을 통한 중공고분자 입자 제조시 중합변수가 입자구조에 미치는 영향
엄기범,권기섭,정재식,우종표 明知大學校 産業技術硏究所 1999 産業技術硏究所論文集 Vol.18 No.-
The polymeric hollow particles with size of ca. 570∼580㎚ and hollow size of ca. 300㎚ can be manufactured using multi-stage emulsion polymerization with methyl methacrylate and methacrylic acid as core monomers and styrene as shell monomer. The morphology of the composite latex particles is dependent on the various polymerization conditions. The viscosity of polymerization loci and the difference of hydrophilicity were found to play an important role in controlling the final morphology. There was no significant difference between power feeding method and step-wise feeding method on the morphology of void latex. However mixture feeding method resulted in no apparent void in swelled latex. The result of opacifying test showed that microvoid film manufactured by using power feeding method and step-wise feeding method had higher hiding power than mixture feeding method. The hiding power of microvoids was increased with increasing pigment weight concentration in water-based paint.
유화중합을 이용한 폴리스티렌 미세다공체의 제조에 관한 연구
김선남,엄기범,우종표 명지대학교 대학원 1997 대학원논문집 Vol.1 No.-
A low density microcellular foam was prepared using inverse emulsion polymerization method. First radically polymerizable styrene monomer and water with nonionic surfactants formed inverse emulsion, then this system was polymerized. The microcellular foam was obtained by removing water phase. The morphology of emulsion foams with system variables was examined in terms of pore size, cell structure, bulk density, and specific surface area. The emulsion system variables including monomer feeding methods, oil phase/water phase ratio, surfactant/water ratio, and mechanical stirring conditions were consider in this study. The ratio of surfactant/styrene rather than that of styrene/water shows dominant effect on the pore size of emulsion foams. While the open-cell/closed-cell morphology was mainly determined by the ratio of styrene/water. Mechanical stirring also has an effect on the pore size. Sorbitan monooleate among various nonionic surfactants seems to be the best surface active agent in styrene/water inverse emulsion system.