방사선에 의한 간독성에 있어서 홍삼과 복합 비타민이 방호효과에 미치는 영향

안승주 ( Seung-ju Ahn ) 한국고등직업교육학회 2013 한국고등직업교육학회논문집 Vol.14 No.2

When organisms exposed to the radiation, macromolecules and water molecules that make up cells by the activeoxygen act physically and then free radical is generated. Finally biological effects of the acute and chronic disorder is known to appear. This study was conducted in order to compare the differences of hepatotoxic protective effect of red ginseng that is in the limelight for its radiation protection effect and multivitamin that has antioxidant effect. 20 male Spague-Dawley rats each 4 Control group, R(radiation) group, RG group(radiation+ginseng), RV group(radiation+multivitamins) , RGV group(radiation+Red ginseng+multivitamins) in group 5 divided into normal control group except for the 4 groups before the experimental 6Mev 0.5Gy 1 times irradiated. Red ginseng administered orally every 15 days by mixing with water and the 1:10, the multivitamin administered percutaneously once a week for 15 days use to 1cc syringe. CBC test in the blood test did not differ in the particular. On clinical chemistry tests, measured result of serum AST, ALT, GGT, T-CHO for liver function tests was able to confirm that relative R group(radiation) is higher than in the other groups, and RG group(radiation+ginseng), RV group(radiation+complex vitamins) was able to confirmed that the decrease. On RGV group(radiation+Ginseng+multivitamins) administered with red ginseng and vitamins, the RG group and RV group was able to confirmed relatively lower measurement than RGV group.

카드뮴 중독 흰쥐에 대한 Noninvasive Biomarker로서의 요중 Ascorbic Acid에 관한 연구

안승주(Seung-Ju Ahn),김용호(Yong-Ho Kim),류재두(Jai-Du Yoo),이용우(Yong-Woo Lee) 대한의생명과학회 1997 Biomedical Science Letters Vol.3 No.2

본 연구는 카드뮴의 이용 및 환경오염의 증가로 일어날 수 있는 카드뮴 중독에 대한 새로운 생물학적 지표로서의 요중 ascorbic acid를 평가하기 위하여 실험적으로 중독시킨 Sprague-Dawley 종의 흰쥐를 이용하여 요중 ascorbic acid 측정, 신장의 조직병리학적 조사 및 생화학적 간 기능 검사를 실시하였다. 측정된 요중 ascorbic acid 농도의 전 실험기간 (50일) 평균치는 실험군I (카드뮴 100 ppm)이 214.0 ㎎/dl 실험군Ⅱ (카드뮴 200 ppm)가 254.3 ㎎/dl로 대조군 9.0 ㎎/dl에 비해 각각 24배 및 28배의 증가를 보였다. 조직병리학적 소견으로는 신장의 근위 세뇨관에서 단백뇨의 원인으로 추정되는 호산성 초자양 물질이 관찰되어 신장의 손상이 있었고, 생화학적 분석에서 실험군I 에서 AST, ALT의 수치가 대조군의 143 ㎎/dl, 50 ㎎/dl에 비해 각각 199 ㎎/dl, 88 ㎎/dl, 실험군Ⅱ가 270 ㎎/dl, 226 ㎎/dl로 나타나 간 기능의 손상이 있었음을 알 수 있었다. 이상의 결과로 실험동물이 카드늄에 노출됨에 따라 그 아만성 독성으로서 간장 및 신장 기능의 손상이 있었고 또한 요중의 ascorbic acid가 현저하게 증가되어 요중 ascorbic acid가 실험동물의 카드늄 노출에 대한 noninvasive 진단 지표로서 활용될 수 있을 것으로 기대된다. This study was planned to evaluate the urinary ascorbic acid as a new biological marker for the intoxication of cadmium, which could possibly be driven by its increased utilization and environmental pollution. In order to meet this goal, we have performed measurement of urinary ascorbic acid concentration, histopathological examination of the kidney, and biochemical test for the liver function using cadmium-intoxicated rats by oral administration. The average concentrations of urinary ascorbic acid in the CdCl₂-treated rats were 214.0 ㎎/dl for 100 ppm group and 254.3 ㎎/dl for 200 ppm group during experimental period of 50 days. These levels are 24 and 28 times higher than one in the control group (9.0 ㎎/dl), respectively. Ultrastructural study showed the eosinophilic hyaline cast and focal effacement, fusion in the renal tubules, as well as loss of foot processes on the glomerular epithelial cells. These results suggested that cadmium may be responsible for renal glomerular injury. The blood levels of AST, ALT and LDH in the treated groups (199 IU/l, 88 IU/l, 1190 U/l for the 100 ppm group and 270 IU/l, 226 IU/l, 760 U/l for the 200 ppm group) were higher than ones in the control group(143 IU/l, 50 IU/l, 334 U/l). These results indicated the cadmium induced the damage of liver function. In conclusion, the administration of cadmium showed a remarkable increase of urinary ascorbic acid with renal and hepatic damage. Therefore, it is expected that measurement of urinary ascorbic acid would be an powerful method as a noninvasive biomarker for cadmium intoxication.

객담검체의 단순도말과 점액제거 도말법에 따른 세포학적 유용성에 관한 비교

안승주 ( Seung-ju Ahn ),현혜진 ( Hey-jin Hyun ) 한국고등직업교육학회 2015 한국고등직업교육학회논문집 Vol.16 No.1

To diagnose respiratory cytological analysis, sputum examination is a common method. The purpose of the sputum examination is the prediagnosis of the various respiratory system diseases, and diagnosis of lung cancer through detection of cancer cells. The purpose of this study was Saccomanno's method is more useful than the direct smear by comparing swept away against a slide for the removal of mucus and efficiency of cytologic examination from removing unnecessary mucous. Considering options are the amount of mucus, presence of macrophages, fixed state for observing the ratio of cell damage. The result of the experiment is the direct smear remains viscosity. One the contrary, Sacommano's method seems it removes viscosity well. The reagent of Sacommano's method has great efficiency of removing the viscosity and it doesn't affect any damages on colors or shapes of epithelial tissues. Also, it has more amount of macrophage. Therefore, when microscopic examination is needed, using Saccomanno's method is very useful to examine the specimen like sputum which has a lot of viscosity.

인체해부학 수업에서 뼈 조직해부학 실습의 학생선호도 조사에 관한 연구

안승주 ( Seung-ju Ahn ),정세훈 ( Se-hoon Jung ) 한국고등직업교육학회 2016 한국고등직업교육학회논문집 Vol.17 No.1

The purpose of this study was to investigate the educational efficacy of online image and a trial perfomance(OIA) on a student group who are studying bone tissue anatomy in a clinical laboratory science course. The survey was conducted on 53 clinical laboratory science students who completed human anatomy. The questionnaire asked students to compare their academic achievement with or without watching OIA before class. Large number of students answered that OIA was indeed helpful for their study understanding the three dimensional of anatomical structure and absorbing concept and knowledge. Some of students requested to extend the application of OIA in their class in the future. Based on the results, it is highly recommended to use OIA in the anatomy class.

폐플라스틱을 활용한 골재의 표면가공 효과에 대한 연구

안승주(Ahn, Seung Ju),리아나(Satlykova, Liana),노영숙(Roh, Young Sook) 한국콘크리트학회 2023 한국콘크리트학회 학술대회 논문집 Vol.35 No.1

양서류(Hynobius leechii)부화에 저선량 방사선조사가 미치는 영향

안승주 ( Seung-ju Ahn ),김구환 ( Kgu-hwan Kim ) 한국고등직업교육학회 2010 한국고등직업교육학회논문집 Vol.11 No.4

Radiation interaction pathways such as to break chemical bonds in materials or to disrupt the structure of the DNA can produce damage to the DNA of irradiated cells and the damaged cells may occur the acute radiation disorders. In this study, we identified the reason for decrease survival rates in the case group was the radiation effects, even though salamander exhibited the differences in survival and breeding rates by amount of radiation. In particular, we confirmed that even the low doses of radiation can cause severe damages to fertilized egg in early cell division.

기온변화가 망월지 두꺼비유생 집단폐사에 미치는 영향

김구환 ( Kgu Hwan Kim ),안승주 ( Seung Ju Ahn ),김수정 ( Su Jung Kim ),박희천 ( Hee Cheon Park ) 한국환경생물학회 2013 환경생물 : 환경생물학회지 Vol.31 No.4

대구의 동남부지역에 위치한 망월지에서 2007년부터 2009년 사이에 일어난 두꺼비 유생의 집단폐사에 관한 연구이다. 망월지두꺼비를 대상으로 미생물학적 조직학적 검사를 시행하여 Aeromaona hydrophila를 분리해낼수 있었다. A. hydrophila는 수계환경에 광범위하게 분포하는 기회감염균이다. 2007년~2009년 상승된 기온변화가 망월지내의 수온을 상승시킴으로서 어패류 및 양서류의 병원성 세균인 A. hydrophila의 급속한 증식이 유발되어 두꺼비유생을 감염시켜 집단폐사로 이어진 것으로 사료된다. We have confirmed the first case of mass mortality which occurred in the wild populations of larval toad, Bufo bufo gargarizans during 2007~2009 at Mangwel pond, which is a long time breeding site, and is located at the south eastern area in Daegu. We have investigated through microbiological tests the cause of mass decease of the larval toad, and detected numerous bacteria from the intestine by histological examines and cultures. The pathogen isolated from the infected larval toads has been identified as Aeromonas hydrophila, which is widely distributed in aquatic environment. However, this phenomenon of mass lethal pattern of the larval toad disappeared at the breeding pond after maintaining better water levels and low temperature in spring during breeding season in 2010. We propose that the high temperatures during the 2007~2009 breeding season resulted in lower water levels and drier conditions leading to explosive propagation of A. hydrophila in the pond which impacted the larval toad`s immune function.

임상병리과 임상실습에 대한 병원실습지도자 및 학생의 의식조사

도성탁 ( Seong-tak Doh ),권순일 ( Sun-il Kwon ),안승주 ( Seung-ju Ahn ) 한국고등직업교육학회 2008 한국고등직업교육학회논문집 Vol.9 No.4

임상병리과의 임상실습에 대한 의식조사로서 병원관계자 36명 및 대구보건대학 임상병리과 3학년 재학생 중 임상실습을 마친 148명을 대상으로 하여 2007년 5월부터 2007년 6월까지 설문지를 배부하여 조사한 결과는 다음과 같다. 임상실습기간에 대해 병원관계자의 경우 80.6%, 학생은 65.8%가 적절하다고 응답하였으나, 늘려야 한다는 의견도 각각 19.4%, 21.2%가 되었다. 실습 내용면에서 학생들의 34.5%가 실제업무위주로 이루어졌다고 한 반면, 60.8%가 단순한 보조업무였다고 답하였다. 병원 측의 30.6%가 체계적인 실습프로그램이 없다고 응답하였다. 실습지도자의 성의에 만족하는가하는 질문에 보통이상이라고 답한 학생 90.5%, 불만족이라는 학생 9.5%이었는데, 이는 성심성의껏 지도하였다는 병원 측의 94.1%와 그렇지 않다 5.9%와 대체적으로 일치하였다. 임상실습 후 실습에 대한 만족도 조사에서 91.7%가 보통이상이라고 답하였고, 실습 후 자신감을 얻었다고 75.7%가 응답하였다. 위에서 보는바와 같이 학생들은 임상실습을 통해 자신감을 쌓아가는 긍정적인 반응을 보였다. 그러나 체계적인 계획에 의거한 교육가 평가가 병원마다 차이를 보이고 있어 구체적인 실습지침의 필요하다 하겠다. Hospital practice program (HPP, Im-Sang-Sil-Seup) is an important part of current curriculum of various health- related academic majors of Korean health science colleges. This survey aims to study the satisfaction level of students who have completed HPP. The respondents to the questionnaire consist of 148 senior students who did HPP and 36 hospital staffs who actually led HPP for the students. As for the length of HPP, the 65.8% of students and 80.6% of hospital staffs said current 8 week period was appropriate to achieve the educational goal of HPP. In contrast, 21.2% of students and 19.4% of hospital staffs thought longer period was needed to learn various important topics during HPP. While 34.5% of students said their HPP in hospital was focused on valuable practical works, as much as 60.8% of students thought their role was merely simple temporary assisting work forces in hospitals. The 30.6% of respondent hospital staffs admitted that they did not have any well organized training program for HPP. With regard to satisfaction level to the sincerity of trainer of HPP in the hospitals, 90.5% of students were positioned in the middle or satisfied scale, and 9.5% of students were in dissatisfied scale. These figures are in good accordance with the answer of hospital staffs that they taught the students in all sincerity by 61.8%, and in the middle by 32.4%. The 91.7% of students were in the middle or satisfied scale in terms of the satisfaction level on whole HPP implementation. The 76.8% of students said they gained self-confidence after completing HPP. The overall assessment on HPP by students was rather positive. HPP provided with a good chance for students to learn practical clinical works and to build self-confidence for their vocational tasks. However, depending on hospital condition, training program and evaluation system was quite variable. A detailed and standardized training guide for HPP is recommended to be prepared to assure a guaranteed quality education.

생체물질의 마이크로파를 이용한 물리적 고정과 화학적 고정에 대한 비교 연구

안승주 대구보건전문대학 2007 대구보건대학 論文集 Vol.27 No.-

Heat treatment causes partial denaturation of protein, which is one of basic principles of histological fixation. Application of conventional heating method like boiling is limited to small specimens due to its poor heat conduction in biological materials. Microwave heating method could be a good alternative to the boiling method. Microwave energy can be used for biological sample fixation, and is known to be as effective as or better than chemical fixation. In this study, the optimum condition for the fixation of rat kidney with microwave was obtained at 62±2℃(in 10 ㎖ N.B.F, 13 sec irradiation with a domestic microwave). The microwaved specimens were embedded in paraffin and sectioned. The quality of the section was improved in histochemical reaction when compared with the specimens fixed with other methods. The total duration engaged in the preparation with microwavefixed specimen was 16 hours, which is shorter than that of traditional methods.

카드뮴 중독 흰쥐에 대한 Noninvasive Biomarker로서의 요중 Ascorbic Acid에 관한 연구

이용우,김용호,안승주,류재두 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 1997 Journal of biomedical laboratory sciences Vol.3 No.2

본 연구는 카드뮴의 이용 및 환경오염의 증가로 일어날 수 있는 카드뮴 중독에 대한 새로운 생물학적 지표로서의 요중 ascorbic acid를 평가하기 위하여 실험적으로 중독시킨 Sprague-Dawley종의 흰쥐를 이용하여 요중 ascorbic acid측정, 신장의 조직병리학적 조사 및 생화학적 간 기능 검사를 실시하였다. 측정된 요중 ascorbic acid농도의 전 실험기간 (50일) 평균치는 실험군Ⅰ(카드뮴 100ppm)이 214.0㎎/dl, 실험군Ⅱ(카드뮴 200ppm)가 254.3㎎/dl로 대조군 9.0㎎/dl에 비해 각각 24배 및 28배의 증가를 보였다. 조직병리학적 소견으로는 신장의 근위 세뇨관에서 단백뇨의 원인으로 추정되는 호산성 초자양 물질이 관찰되어 신장의 손상이 있었고, 생화학적 분석에서 실험군Ⅰ에서 AST, ALT의 수치가 대조군의 143㎎/dl, 50㎎/dl에 비해 각각 199㎎/dl, 88㎎/dl, 실험군Ⅱ가 270㎎/dl,226㎎/dl로 나타나 간 기능의 손상이 있었음을 알 수 있었다. 이상의 결과로 실험동물이 카드뮴에 노출됨에 따라 그 아만성 독성으로서 간장 및 신장 기능의 손상이 있었고 또한 요중의 ascorbic acid가 현저하게 증가되어 요중 ascorbic acid가 실험동물의 카드뮴 노출에 대한 noninvasive진단 지표로서 활용될 수 있을 것으로 기대된다. This study was planned to evaluate the urinary ascorbic acid as a new bilolgical marker for the intoxication of cadmium, which could possibly be driven by its increased utilization and environmental pollution. In order to meet this goal, we have performed measurement of urinary ascorbic acid concentration, histopathological examination of the kidney, and biochemical test for the liver function using cadmium-intoxicated rats by oral administration. The average concentrations of urinary ascorbic acid in the CdCl₂-treated rats were 214.0㎎/dl for 100ppm group and 254.3㎎/dl for 200ppm group during experimental period of 50 days. These levels are 24 and 28 times higher than one in the control group (9.0㎎/dl), respectively. Ultrastructural study showed the eosinophilic hyaline cast and focal effacement, fusion in the renal tubules, as well as loss of foot processes on the glomerular epithelial cells. These results suggested that cadmium may be responsible for renal glomerular injury. The blood levels of AST, ALT and LDH in the treated groups (199 IU/I, 88 IU/I, 1190 U/I for the 100 ppm group and 270 IU/I, 226 IU/I, 760 U/I for the 200 ppm group) were higher than ones in the control group(143 IU/I, 50 IU/I, 334 U/I). These results indicated the cadmium induced the damage of liver function. In conclusion, the administration of cadmium showed a remarkable increase of urinary ascorbic acid with renal and hepatic damage. Therefore, it is expected that measurement of urinary ascorbic acid would be an powerful method as a noninvasive biomarker for cadmium intoxication.