미국 상위 30개 연구 중심 대학들의 실험실 환경보건안전 담당 부서 -MIT 사례를 중심으로-

안광석 ( Kwang Seog Ahn ),강동묵 ( Dong Mug Kang ),신용철 ( Yong Chul Shin ),전영희 ( Young Hee Jun ) 한국산업위생학회 2007 한국산업보건학회지 Vol.17 No.3

Objectives: The objectives of this research are to examine the missions, organization, and programs of the environmental, health and safety(EHS) offices of the major research universities in the U.S.A., particularly the case of Massachusetts Institute of Technology(MIT) EHS Office, and to suggest ways to address the EHS issues of the universities and research institutes in South Korea. Methods: The top 30 research universities in the U.S.A. were selected by the total amounts of research funds they annually received. The web sites of the 30 universities were searched to identify the names of the departments that address environment, health, and/or safety related issues, the missions of those departments, the major programs of those departments, and the number of staff in those departments. Also, a case study was conducted for the Environment Health and Safety Management System and the Environment, Health and Safety Office of the MIT, using literature and web searches and a meeting with the Director of the MIT Environment, Health and Safety Office. Results: All the top 30 research universities in the U.S.A. had designated departments that dealt with EHS issues. Most of them were by the name of or similar to environmental, health and safety. The mission statements of those departments were mostly about improving the safety and health of their community members, improving the environment, and complying to EHS regulations. Most of those departments had an environmental management program, industrial hygiene program, radiation protection program, safety program, and biosafety program as their major programs. The components of the environment, health and safety management system(EHS-MS) of the MIT were examined in detail. In contrast, not many universities in South Korea had designated departments that dealt specifically with EHS issues. Also, the number of fulltime staff for EHS was only 1-2 in most of the Korean universities and their work duties included only general safety, while neglecting other health/environment related issues. Conclusions: Well organized and functioning environmental, health and safety offices were present in all of the top 30 research universities in the U.S.A., whereas similar organizations of the universities in South Korea were virtually non-existent and/or had very limited EHS activities. Therefore, in order to reduce potential risks of accidents and health problems in the Korean universities and research institutions, well established and functioning EHS-MSs and EHS offices are warranted. The case of the EHS-MS and EHS Office in the MIT demonstrate a successful case to follow.

소음주의구역 (Noise Perimeter Zones)-고속도로 신설 공사 건설 현장에서의 소음 노출 저감을 위한 새로운 체계적인 방법

안광석 ( Kwang Seog Ahn ),( Susan Moir ) 한국산업위생학회 2006 한국산업보건학회지 Vol.16 No.2

이 연구에서는 고속도로 신설 공사 건설 현장 근로자들의 소음 노출을 저감할 수 있는 방법들을 제시하며 특히 현장에서 적용할 수 있는 새로운 체계적인 방법인 "소음 주의 구역"을 제안한다. 고속도로 건설 현장에서 소음원이 되는 장비, 기계, 도구와 근로자들의 소음 노출 특성을 알아 보았으며, 개인청력 보호구, 공학적제어수단(engineering controls), 관리적제어수단(administrative controls)을 어떻게 적용할 것인가를 제시하였다. 개인청력 보호구만으로는 소음 노출을 효율적으로 저감시킬 수 없음을 보였으며, 공학적제어수단은 기술적 및 경제적 문제로, 관리적제어수단은 공사 기간 단축과 같은 실제적인 이유로 해서 시행 되기가 어렵다. 따라서 이 연구에서는 실제적으로 적용할 수 있는 체계적인 방법으로서 "소음주의구역(Noise Perimeter Zones)"을 새롭게 제안하였다. 이 "소음주의구역"은 높은 수준의 소음원이 존재하는 고속도로 건설 공사 현장에 지정된다. 이 구역안에서는 근로자들의 출입이 통제되며 또한 개인청력 보호구, 공학적제어수단, 관리적제어수단과 같은 적절한 제어수단이 사용되어야 한다.

이용질계(二溶質系)에서 Trihalomethane 의 흡착특성(吸着特性)

정태학,안광석,Chung, Tai Hak,Ahn, Kwang Seog 대한토목학회 1987 대한토목학회논문집 Vol.7 No.2

활성탄을 이용하여 chloroform, 4 염화탄소 및 crystal violet의 흡착특성(吸着特性)을 단일용질계(單一溶質系)와 2 용질계(溶質系)에서 연구(硏究)하였다. 단일용질계(單一溶質系)에서 각 용질(溶質)의 흡착(吸着)은 Freundlich 식(式)에 의해 적절히 표현되었다. 2 용질계(溶質系)에서의 흡착(吸着)의 농도(濃度)가 1 mg/l 이하의 낮은 농도(濃度)에서도 심한 조해현상(阻害現狀)을 나타냈다. chloroform의 흡착시(吸着時) 화학구조가 유사(類似)한 4 염화탄소가 화학구조가 상이(相異)한 crystal violet 에 비해 훨씬 큰 영향을 미쳤다. 조해효과(阻害效果)는 crystal violet 흡착시(吸着時) chloroform 에 의한 것이 가장 높았으며 chloroform 과 4 염화탄소가 서로 미치는 영향은 대등하였다. 2 용질계(溶質系)는 Fritz 와 Schl${\ddot{u}}$nder 모형(模型)에 의해 적절히 표현(表現)할 수 있었고 훨씬 간단한 3 상수(常數) Freundlich 식(式)에 의해 표현가능(表現可能)하였다. Adsorption characteristics of chloroform, carbon tetrachloride, and crystal violet were investigated in single-and bi-solute systems. Single-solute adsorption for each solute was well described by Freundlich equation. Severe inhibition was recorded in bi-solute adsorption systems despite of low solute concentration of less than 1 mg/l. Inhibition of chloroform adsorption by carbon tetrachloride, similar compound in chemical structure, was much higher than by crystal violet of which chemical structure is quite different. Highest inhibition was observed at crystal violet adsorption by chloroform. While, inhibition caused by each other was almost equal between chloroform and carbon tetrachloride. Bi-solute adsorption was well described by Friz-Schl${\ddot{u}}$nder model and by much simpler 3 parameter Freundlich equation.

Symposia / S1-2 : Role of HLA-G in Protecting Human Placenta Cells from Natural Killer Cytolysis

(Young Soo Jun),(Eun Ok Kim),안광석(Kwang Seog Ahn) 한국생화학분자생물학회 (구 한국생화학회) 1999 생화학분자생물학회 춘계학술발표논문집 Vol.- No.-

박테리아의 유전적 요소가 공생주인 Amoeba proteus 의 핵의 형태 유지에 미치는 영향

안태인,안광석 한국유전학회 1987 Genes & Genomics Vol.9 No.1

XD strain of Amoeba proteus which was harvoring the symbiotic bacteria was cultured in the medium containing antibiotics to repress the bacterial gene expression or to cure the plasmids from the bacteria. Effects of these antibiotics or thermal treatment on the morphology of the host nucleoli were followed by methylgreen staining. The apparent effects of those antibiotics blocking transcription or translation of the prokaryotes or that of thermal treatment were the loss of the bacteria from the cytoplasm and formation of clumped nucleoli. By treatment with acridine orange or ethidium bromide the nucleoli also clumped to form large aggregates and the population of the symbionts reduced to the 60% level of the control. When the plasmids of the symbionts were cured the amoebae were unable to recover from the abnormality of the nucleolus morphology. In the newly infected tD strain, it took 35 days to aquire the characteristics of xD strain such as susceptibility to antibiotics and reversibility from the effects of antibiotics. These results support the view that the encoded informations on the plasmids of the symbiotic bacteria are responsible for the maintenance of normal morphology of the host nucleoli.

Amoeba proteus 공생주 xD strain 에 있어서 박테리아 유전 기구의 역할 : 항생제를 이용한 분석 an Analysis Using Antibiotics

안태인,안광석 한국유전학회 1989 Genes & Genomics Vol.11 No.2

Roles of the bacterial genetic components in the endocellular symbiosis of xD strain of A. proteus were determined by application of plasmid curing agents and antibiotics. The effects of plasmid curing or blocking prokaryotic gene expression were analysed by two dimensional gel electrophoresis, autoradiography and experimental infection. Curing plasmids of the bacteria resulted in loss of the ability to infect the new host. By selective suppression of the transcription or translation of the symbiotic amoebae it was possible to localize the gene of xD strain specific protein in the bacterial chromosome. When the gene expression of bacterial chromosome was blocked, the symbiotic vesicles underwent degradation in xD strain and the bacteria were unable to develop the symbiotic vesicles in the newly infected host. Thus the development and maintenance of the symbiotic vesicle are under the control of the bacterial genome. Plasmids of the symbionts were found to be essential for the initial step of symbiosis by enabling the bacteria to avoid digestion in phagolysosomes.