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사염화탄소와 에탄올로 유발된 간손상에 대한 GODEX<SUP>Ⓡ</SUP>의 치료효과 및 투여 중단에 따른 효소 역전 현상의 비교 평가
신지순(Ji Soon Sin),정은용(Eun Yong Jung),채희열(Hee-Youl Chai),권운(Woon Kwon),최은경(Ehn Kyoung Choi),조영민(Young Min Cho),황석연(Seok-Yeon Hwang),이민호(Min Ho Lee),김윤배(Yun-Bae Kim),강종구(Jong-Koo Kang) 한국실험동물학회 2003 Laboratory Animal Research Vol.19 No.4
Cephalosporin계 항생제 IDC7181에 대한 항원성시험
신지순(Ji Soon Sin),장호송(Hu-Song Zhang),최은경(Ehn-Kyoung Choi),권운(Woon Kwon),채희열(Hee-Youl Chai),조영민(Young Min Cho),김윤배(Yun-Bae Kim),강종구(Jong-Koo Kang) 한국독성학회 2003 Toxicological Research Vol.19 No.4
Antigenic potential of a novel cephalosporin, IDC7181 was examined a active systemic anaphylaxis (ASA) and passive cutaneous anaphylaxis (PCA) test, in guinea pig and mouse-rat models, respectively. In ASA test, IDC7181 induced the signs of restlessness in a few animals immunized with a high dose (100 mg/kg) of IDC7181 alone or in combination with Freund's complete adjuvant (FCA). In PCA test, only one of ten sera from the animals immunized with a high dose (100 mg/kg) of IDC7181 in the absence or presence of FCA showed positive reaction. The positive reaction,<br/> induced by IDC7181, which may be due to β-lactam ring, in ASA and PCA tests were negligible in comparison with those of traditional cephalosporins. Taken together, it is suggested that IDC7181 do<br/> not cause immunological problems in clinical dosage.
사염화탄소로 유발된 간손상에 대한 구기자 추출물의 예방효과
조정희(Jung-Hee Cho),신지순(Ji Soon Sin),김은주(Eun Jun Kim),신선희(Sunhee Shin),장자영(Ja Young Jang),신경섭(Kyeong Seob Shin),김윤배(Yun-Bae Kim),강종구(Jong-Koo Kang),황석연(Seock-Yeon Hwang) 한국실험동물학회 2004 Laboratory Animal Research Vol.20 No.2
The protective effects of the extract of Lycii fructus against hepatotoxicity of carbon tetrachloride (CCl₄) were studied in rats. The rats were orally treated with CCl₄ (50% in com oil) at initial dose of 1 ㎖/㎏ followed by 0.5 ㎖/㎏ four times during 2-week period. The extract of L. fructus (50, 100 or 200 ㎖/㎏) or its vehicle was administered everyday from 1 week before the start of CCl₄ injection for five weeks. Exposure to CCl₄ reduced red blood cells (RBC) and hemoglobin, whereas it markedly enhanced white blood cells (WBC). In addition, CCl₄ induced hepatocelluar degeneration and necrosis, leading to a great increase in serum aspartate aminotransferase (AST), alanine aminotrasferase (ALT) and alkaline phosphatase (ALP) levels. In biochemical analyses, thiobarbituric acid-reactive substances (TBARS) and physphatidylcholine hydroperoxide (PCOOH) in hepatic tissues were remarkably increased by CCl₄ treatment, resulting in the induction of antioxidant enzymes such as catalase and superoxide dismutase (SOD). Noteworthy, such changes in RBC, WBC and hemoglobin were recovered by treatment with the extract of L. fructrus to the control levels. In addition, not only increases in serum AST, ALT and ALP, but also induction of lipid peroxidation and antioxidant enzymes in hepatic tissues caused by CCl₄ were significantly attenuated by the L. fructrus extract in a dose-dependent manner. Such hepato-protective effects of L. fructrus extract were confirmed by histopathological examinations, wherein only mild hepatocytic vacuolations were observed in the liver of rats treated with a high dose (200 ㎎/㎏) of L. fructrus extract, in contrast to severe hepatocytic degenerations in rats administered with CCl₄ alone. Taken together, it is suggested that the extract of Lycii fructus could be a promising candidate for the protection of liver injury, based on the preventive effects against morphological cellular injuries, lipid peroxidation and serum biochemical parameters.
황석연(Seock-Yeon Hwang),신지순(Ji Soon Sin),권운(Woon Kwon),채희열(Hee-Youl Chai),조정희(Jung-Hee Cho),이남진(Nam Jin Lee),박종배(Jong Bae Park),김익수(Iksoo Kim),류강선(Kang Sun Ryu),윤치영(Chi-Young Yun),강종구(Jong-Koo Kang),김윤배 한국실험동물학회 2004 Laboratory Animal Research Vol.20 No.2
The purpose of this study was to confirm the safety of the extract of cricket, Gryllus bimaculatus. Male Sprague-Dawley rats were divided into 5 groups, 10 animals in each group, and orally administered with the cricket extract at doses of 0 (vehicle), 25, 50, 100 or 200 ㎎/㎏ for 2 weeks. In the results, there were no significant differences in the body weight gain between vehicle control and cricket-treated groups. Also, significant changes in daily food intake and water consumption were not observed through tout the experimental period. In addition, hematological parameters did not show any significant or dose-dependent alterations. Serum biochemical parameters including aspartate transaminase, alkaline phosphatase, creatine kinase, cholesterol, triglyceride, lactate dehydrogenase, pancreatic amylase, iron and magnesium decreased at high doses, in contrast to increases in glucose and potassium levels, although such changes were in normal ranges. In gross findings, no remarkable lesions were seen, except a light increase in only absolute weight, but not relative weight, of submaxillary glands at 25 ㎎/㎏ of cricket. In addition, no abnormal histopathological findings were observed. Taken together, it is suggested that repeated treatment with the extract of cricket may not exert considerable side effects.
이종권 ( Jong Kwon Lee ),신지순 ( Ji Soon Sin ),김진호 ( Jin Ho Kim ),엄준호 ( Jun Ho Eom ),김형수 ( Hyung Soo Kim ),박귀례 ( Kui Lea Park ) 대한화장품학회 2005 대한화장품학회지 Vol.31 No.3
화장품은 의약품과 달리 남녀노소를 불문하고 거의 평생에 걸쳐 사용하는 제품이므로 피부 및 인체의 안전성 확보는 중요한 문제라고 할 수 있다. 화장품의 안전성 평가는 화장품법에 식품의약품안전청장이 고시하도록 되어있어 현재 식약청고시 “기능성화장품등의 심사에 관한 규정”에서 정하고 있다. 자외선에 의한 피부독성은 피부노화, 피부홍반, 피부손상, 피부암 유발 면역계장애 등이 있으며, IARC (International agency for research on cancer)에서는 자외선을 사람에게 암을 유발하는 물질로 분류하고 있다. 자외선 노출에 의하여 설치류에 대한 피부암 유발보고는 많이 있고, 현재 전 세계적으로 자외선에 의한 피부 암화 과정에 대하여 활발한 연구가 진행되고 있다. 화장품이 광에 의한 영향을 평가하는 시험은 광독성시험으로, 국내에서 광독성에 대한 규정은 자외선에서 흡수가 없음을 증명하는 흡광도 시험자료를 제출하는 경우는 면제이고 그 외의 경우는 상기 고시에 의한 광독성 시험과 광감작성 시험을 실시하도록 되어 있다. 이 시험은 일반적으로 기니픽 또는 토끼를 포함한 적절한 동물로 실시하도록 규정되어 있다. 화장품 안전성 평가에 있어서 동물대체시험법의 요구는 3R (replacement, refinement, reduction) 운동으로 유럽을 중심으로 시작하여 이제는 전세계로 확대되어 있으며, 화장품 안전성심사에 있어 중요한 이슈로 대두되고 있다 화장품의 광독성 평가에 대한 대체시험법으로 3T3 NRU(neutral red uptake) 광독성시험이 2004년 4월 OECD 독성시험 기준으로 채택되었다. In vitro 광독성 시험법은 마우스 유래의 섬유아세포인 3T3 cell을 이용하여 광조사한 것과 광조사하지 않은 세포와의 세포독성을 NRU 시험을 이용하여 그 정도를 비교하여 그 차이(PIF, photoirritation factor)가 5배 이상이 되면 광독성 물질로 분류하는 평가방법이다. Safety is one of the key issue in the regulation of cosmetics. Cosmetic Act deals with it in Korea. The guidance for the testing cosmetic ingredients and their safety evaluation are prepared by Korea Food and Drug Administration. Ultraviolet radiation could Induce skin damage, edema, erythema, photoaging, immune dysfunction and skin cancer. Ultraviolet radiation is classified as Group 2A(probably carcinogenic to humans) by International Agenry for Reaserch on Cancer(IARC). The in vitro methodologies for evaluating the toxic potential of ingredients reported in the literature have not yet been sufficiently validated for use in areas other than the study for mutagenicity/genotoxicity, for pre-screening for severe irritancy, for screening of phototoxicity and for evaluating the percutaneous absorption. The 3T3 neutral red uptake photoxicity test (3T3 NRU PT) was accepted as OECD toxicity guideline in 2002. The 3T3 NRU PT is an in vitro method based on a comparison of the cytotoxicitv of a chemical when tested in the presence and in the absence of exposure to a non-cytotoxic dose of UVA/visible light.
고지혈증 랫드에서 구기자 추출물이 혈중 지질에 미치는 영향
조정희 ( Jung Hee Cho ),신지순 ( Ji Soon Sin ),배형준 ( Hyung Joon Bae ),김윤배 ( Yun Bae Kim ),강종구 ( Jong Koo Kang ),황석연 ( Seock Yeon Hwang ) 대한임상검사과학회 2004 대한임상검사과학회지(KJCLS) Vol.36 No.1
The purpose of this study was to investigate the effects of Lycii fructus extract on the serum lipids in hyperlipemia rats. The rats were divided five groups including negative control, positive control and treated L. fructus extract at dose of 50, 100, 200 mg/kg. To induce the hyperlipemia, 4 groups of rats, not including the negative control group, were fed experimental diets consisting of cholesterol, lard, corn oil, and normal diet for experiment period. As a result, the L. fructus extract decreased total cholesterol(T. Cho), trigryceride(TG), low density lipoprotein cholesterol(LDL-C), and free fatty acid(FFA) significantly compared with positive control. However, no significant difference was found in high density lipoprotein(HDL-C) and phospholipid(PPL) level. Otherwise, in serum lipid level including, T-Cho, TG, LDL-C, FFA and PPL was decreased dose dependently by treatment of L. fructus extract. But HDL-C was increased by L. fructus extract too. Thereby having beneficial effect on hyperlipermia by influencing the serum lipid change, it is expected that Lycii fructus extract can suppress arteriosclerosis and obesity.
콜레스테롤 함유 식이 랫드에서 감귤껍질추출물 BNs-3 및 BNs-7의 항비만 효과
채희열(Hee-Youl Chai),신지순(Ji-Soon Sin),권운(Woon Kwon),최은경(Ehn-Kyoung Choi),조영민(Young Min Cho),장호송(Hu-Song Zhang),황석연(Seock-Yeon Hwang),복성해(Song-Hae Bok),김윤배(Yun-Bae Kim),강종구(Jong-Koo Kang) 한국독성학회 2003 Toxicological Research Vol.19 No.3
The protective effects of BNs-3 and BNs-7, extracts of citrous orange peel, on the obesity induced by ad libitum feeding a cholesterol-containing diet to rats were investigated. The animals<br/> were fed on the diet including cholesterol (0.5%) with or without the citrous orange peel extracts BNs-3 (5%) and/or BNs-7 (0.1%) for 8 weeks. The ad libitum feeding a diet containing cholesterol to<br/> rats from 6 weeks of age increased the body weight gain compared with that of rats fed on a normal diet. Such an increase in body weights was markedly attenuated by the addition of BNs-3 or BNs-7 to the diet. Especially, a combinational feeding on BNs-3 and BNs-7 significantly reduced the body weight gain below that of normal diet-fed animals. Interestingly, the weights of abdominal adipose tissues surrounding epididymides were greatly reduced by the citrous orange peel extracts, in parallel with the decrease in body weights. In addition, blood concentrations of lipids including cholesterol were also lowered by the combinational treatment with BNs-3 and BNs-7. Taken together, it is suggested that the obesity and overweight produced by unrestricted overfeeding on diet with cholesterol may be partially due to the accumulation of abdominal adipose tissues, around the epididymides in rats, and that citrous orange peel extracts might exert antiobese activities by reducing the adipose tissues as well as blood lipid concentrations.
사염화탄소로 유발된 간손상에 대한 구기자 추출물의 치료효과
조정희 ( Jung Hee Cho ),신지순 ( Ji Soon Sin ),이광주 ( Kwang Joo Lee ),김윤배 ( Yun Bae Kim ),강종구 ( Jong Koo Kang ),황석연 ( Seock Yeon Hwang ) 대한임상검사과학회 2004 대한임상검사과학회지(KJCLS) Vol.36 No.1
The hepatotherapeutic effect of the extract of Lycii fructus has been studied in rats against CCl4 induced liver toxicity. The rats were orally treated with CCl4 (corn oil/CCl4 1:1, 1㎖/㎏) and then CCl4 (0.5㎖/㎏) administered four times for 2 weeks. The extracts of L. fructus have been administered every day for 2 weeks after the last CCl4 injection. The experimental groups consisted of negative control (G1), positive control (CCl4 alone; G2), extract of L. fructus (50 ㎎/㎏; G3, 100 ㎎/㎏; G4, 200 ㎎/㎏; G5), respectively. There was a significant decrement to G2 on the serum level of aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase in G5. Also, the content of thiobarbituric acid reactive substance, and phosphatidylcholine hydroperxidase, a marker of lipid peroxidation, in the liver were decreased significantly G5 and G4 compared with G2. Although, catalase or superoxide dismutase, antioxidant enzyme, in the liver were decreased significantly too, it would not be a good sign for the liver. In histopathological findings, such a hepatocellular vacuolar degeneration, lobular restructure, cellular infiltration, necrosis, and so on were shown severely in G2. However, G4 and G5 was shown a mild cytoplasmic vacuolation and inflammatory cell. In conclusion, as a protection against cell damage, lipid peroxidation and serum level, it suggested that the extract of Lycii fructus would have been a therapeutic effect of liver injury directly.