大豆花粉의 貯藏에 關한 硏究

S.H. KWON(權臣漢),J.H. OH(吳正行),H.S. SONG(宋禧燮) 한국육종학회 1977 한국육종학회지 Vol.9 No.3

The experiment was carried out to determine the germination ability of soybean pollen with different storage conditions. Sucrose-agar media was succeseful for germination of soybean pollen and the germination was hardly affected by the ingredient amounts of agar and sucrose in artificial media. Maximum germination of fresh pollen look place in 80 min. after inoculation of the pollen on 2% agar plate containing 20% sucrose and on difference in germination percent was found between two varieties examined. When soybean pollen was kept at room conditions for 3 hours, the germination averaged less than 10%. However, it was found to be kept higher germination ability of the pollen by storage in brown desiccator being controlled humidity and light. Morever germination could be remarkably improved with storing at the condition of lower temperature and humidity, and the influence of humidity was more conspicuous with long storage hours.

감마線處理에 依한 大豆의 可視的 形態突然變異의 出現率

S.H. KWON(權臣漢),J.L. WON(元鐘樂),H.S. SONG(宋禧燮) 한국육종학회 1981 한국육종학회지 Vol.13 No.2

In order to investigate the frequency of chlorophyll and visible-macro mutations in M₂ population, the air-dried seeds were irradiated with 30kR of gamma-rays. The frequency of chlorophyll-deficient mutation of leaf was about 11% on M₁ plant basis and 1.8% in M₂ seedlings. The rate of visible-macro mutations, such as early and late flowering, shortness, erectoid, rosette, rugose, flower and pubscence color, leaf specks and yellowish-leaf type was about 15.430 and 3.7% on M1 and Ma plant basis, respectively, and the majority of visible macro-mutation types was shortness, early and late flowering, flower color, narrow leaf and rugose types. The mutation rate of two and three characters occurred coincidently in a M₂ plant was about 17.4 based on M₁ mutation plant. Segregation rate of mutants in M₂ averaged about 15% in chlorophyll mutations and 32% in visible-macro mutation types.

감마線을 處理한 大豆 第二世代에서의 突然變異出現率

S.H. KWON(權臣漢),J.L. WON(元鍾樂),H.S. SONG(宋禧燮) 한국육종학회 1981 한국육종학회지 Vol.13 No.2

In order to investigate the radiosensitivity in M₁ and frequency of mutants at seedling stage in M₂ population, the air dried seeds of three varieties were irradiated with gamma-ray doses ranged 10~50kR. The fifty percent decrease due to the radiation effects in plant height at 14 days, survival rate at 40 days after seeding, and number of pods per plant at harvest was ranged from 20 to 30 kR and their varietal differences were not found. Frequency of chlorophyll deficient mutation of leaf was 4~7% in M₁ plant bais and 2~4% in M₂ seedling basis within 20~30kR, and significant differences were not, found among the varieties. Frequency of stem color mutation in Korean native line, KAS 552-4 was higher as compared to that of chlorophyll deficient mutation and the rate was 25% and 7% in M₁ and M₂ basis, respectively. The purpose of experiment was to investigate the radiosensitivity in M₁ generation and frequency of mutants at seedling stage in M₂ population of soybean. The air dried seeds of three varieties, Chungbuk-Baik, KAS 552-4 and CB-27 were irradiated with gamma-ray doses of 10, 20, 30, 40 and 50kR. The results obtained are summarized as follows: 1. The fifty percent decrease due to the radiation effects in plant height at 14 days after seeding, survival rate at 40 days after seeding, and number of pods per plant at harvest was ranged from 20 to 30kR and their varietal differences were not found. 2. Mutant types at M₂ seedling stage were classified into chlorophyll deficiency of leaf, stem color at cotyledon stage, rosette type, twin stem, narrow leaf and multi-leaflets types. 3. Frequency of chlorophyll deficient mutation of leaf with xantha, chlorina, chlorotica and maculata type was 4~7% in M₁ plant basis and 2~4% in M₂ seedling basis within 20~30kR, and significant difference were not found among the varieties employed 4. Frequency of green stem color mutation in Korean native line, KAS 552-4 with purple stem at cotyledon stage was higher as compared to that of chlorophyll deficient mutation and the rate was about 25% and 7% in M₁ and M₂ basis, respectively.

在來栽培種 大豆의 形質變異와 形質間의 相關

S. H. Kwon(權臣漢),K. H. Im(任建爀),J. R. Kim(金在利),H. S. Song(宋禧燮) 한국육종학회 1972 한국육종학회지 Vol.4 No.2

The soybeans, currently grown by Korean farmers, have been grown for many centuries without artificial improvement and it may result an accumulation in abundance of variances. In order to collect the gene sources and to select superior lines from the land races, a continuous collection was made in all part of the country and planted on line bases to study the character and interrelationships among the characters. The average flowering dates were 67 days in the collected population which is earlier than the one studied in the named local varieties. High correlation coefficient was observed between flowering and maturity. Negative correlations between seed yield and maturity or flowering were found, which is contradictory to other reports. The maturity of this population was ranged from 106 days to 151 days and highly correlated with plant height, number of nodes per plant and seed weight. Average plant height in the collected population was small as compare to the average height of named local varieties. Variance for lodging and disease were relatively large. Consequently, there are some possibilities to obtain useful gene sources for resistant to lodging and diseases. Protein content showed negative correlations with oil content, seed yield, flowering and seed weight, whereas positive correlations with plant height and maturity were observed. Since the small plot size for yield estimate the result was not fully reliable, but the relative seed yield among the collected lines ranged from 60 to 3,484 ㎏/㏊ and it is meaningful in breeding for high yielding varieties.

在來種大豆의 主要 形質特性

S.H. Kwon(權臣漢),J.R. Kim,(金在利)H.S. Song(宋禧燮),K.H. Im(任建爀) 한국육종학회 1974 한국육종학회지 Vol.6 No.1

782 local soybean lines currently grown by Korean farmers were collected in the Korean peninsula and studied their important agronomic traits for two years grown at the Experimental Farm of the Korea Atomic Energy Research Institute in Seoul. About 13 percent of these collected lines have produced seeds over 1,000㎏ per hectar and some of them wee apparently superior to the leading varieties grown in the identical conditions. Maximum seed size within this collected population was 42.4g per 100 seeds and 17 percent of this population posesses of seed size over .30 g/100seed. 66 percent of the population was belong to the maturity group IV and V and maturity group 00 and over VII were not found from the collection. Plant height was ranged from 126㎝ to 45㎝ and frequency distribution for plant height shows normal curve. Seed yield possitively correlated with seed size, while negatively associated with flowering, maturity, and plant height.

우리나라 在來種 蒐集 大豆의 蛋白質 및 脂肪含量에 關한 硏究 Ⅱ

S. H. Kwon(權臣漢),J. H. Oh(吳正行),J. R. Kim(金在利),H. S. Song(宋禧燮),B. W. Kim(金炳友) 한국육종학회 1975 한국육종학회지 Vol.7 No.1

A collection of 840 native soybean lines currently grown by farmers was made from the southern part of Korean peninsula. The variability of chemical contents in seeds and relationships between chemical contents and important agronomic traits were studied. 1. The variability for both protein and oil contents are 33.2%-49.8% and 11.2%-23.4%, respectively. The population consisted with over 60 percent of lines containing more than 41% of protein, while approximately 85% of lines belong to low oil category ranged from 15 to 19 percent of oil. 2. It was discouraged in finding marker genes for high protein and high oil content in this investigation. Therefore, appropriate methods of analysis for protein and oil are urgently needed to develop for screening large number of strains in early stages. 3. Sixty six high protein lines (over 45 percent and eight high oil content lines (over 21 percent) were found from the collection. After advanced test, the selected lines will be decided to utilize breeding materials for high protein and high oil.

在來栽培種 大豆의 成熟群別 形質間의 相關

S. H. Kwon(權臣漢),H. S. Song(宋禧燮),H. W. Kim(金昊元),K. H. Lee(李庚熙) 한국육종학회 1974 한국육종학회지 Vol.6 No.2

Eight hundred fourty native soybean lines, collected throughout the Korean peninsula, were grown a field in the vicinity of Seoul City, and studied their maturity and relationshipes among the important agronmic characters within the maturity group. 1. The collected lines were classified according to the maturity. The distribution of maturity groups were 0.1% for group 0, 0.6% for group I, 8.9% for group II, 20.1% for group III, 24.5% for group IV, 34.4% for group V, and 11.4% for group VI. These results indicats that majority of Korean native soybeans are belong to maturity groups III and IV. 2. The majoity of the collected lines from the Gyeonggi Province were earlier in maturity as compare to the lines collected from the other provinces. The collected lines from the provinces of the lower latitude tend to late in maturity. 3. The maturity appeared in large deviation ranging from 112 clays to 160 days. However, either extremely early or late maturity lines from this population were found in a small number. Correlation between maturity and seed yield was shown in negative.

12. 收穫後 Calcium 浸漬方法이 사과 果实의 部位別 Calcium含量에 미치는 영향

임열재(Y.J.Yim),최종승(J.S.Choi),송희섭(H.S.Song) 한국원예학회 1988 한국원예학회 학술발표요지 Vol.6 No.2

한국산 재래콩 및 야생콩 유전 변이

송희섭,강정훈,김남수,최익영 한국콩연구회 1999 韓國콩硏究會誌 Vol.16 No.1

Genetic variations among the Korean soybean germplasm were surveyed using several molelcuar markers. In the studies of 38 different soybean genotypes including 18 Glycine max and 20 Glycine soja, the overall genetic variation among the wild soybean was greater and wider than that of cultivating soybeans. In the cluster analysis, wild germplasm was separated from cultivating soybeans at 83% similarity level. Morphologically intermediate typed soybeans, Glycine gracilis, between wild soybeans and cultivating soybeans were also clustered between these two ecotype soybeans. Genetic diversity and differentiation among the 57 wild soybean accessions collected from riversides of five major rivers showed significantly higher generic diversity and differentiation than those of cultivated soybeans by SSR (simple sequence repeat) variation. There was no specific SSR alleles to the populations from any riversides, which might indicate the present genetic variation among the wild soybeans could predate the dispersal of wild soybeans along the rivers. While the wild soybeans from Nakdong river showed highest level of genetic diversity, the soybeans from Sumjin river showed the highest genetic differentiation value in the comparison of genetic diversities among the populations from each riverside.

大豆 交配后代에 있어서 몇가지 形質의 遺傳的分離

李榮日,宋禧燮,申仁澈,權臣漢 경희대학교 부설 식량자원개발연구소 1986 硏究論文集 Vol.7 No.-

Genetic studies of several traits in the crosses of soybean cultivars were conducted to determine the performance of F₁ hybrids and the segregation pattern in F₂ generation for improvement of vegetable soybean cultivars. Plant height and number of pods per F₁ hybrid increased in comparison with their parents, while flowering of F₁ hybrid was intermediate between early and late flowering time of their parents. Stem and flower color of F₁ hybrid were purple and pubescence color was tawny. In F₂ generation, purple stem against green in the crosses showed 3:l ratio segregation. However, purple flower and tawny pubescence were not fitted to 3:l ratio. Flowering time of F₂ generation in two crosses were segregated with 3:l ratio to early and late flowering time. However, the occurrence of earliness was a deficience to lateness. Numbers of nodes and pods per F₂ plants were distributed between those of their two parents.