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Tributyltin에 의한 흰쥐 정소 내 간질세포의 지방세포 유도와 세포자연사 증가
송연화,정지은,이현아,홍지희,양현원 한국발생생물학회 2010 발생과 생식 Vol.14 No.4
Tributyltin (TBT), an endocrine disrupting chemical, has been reported to decrease testicular function by causing apoptosis in the testis, but this mechanism is not fully understood. Thus, in this study we examined whether TBT induces adipogenesis of the Leydig cells to find out the correlation between adipogenesis and apoptosis in the testis. Three week old SD male rats were orally administrated with sesame oil, 1 ㎎/㎏ of TBT, or 10 mg/㎏ of TBT daily for 1 week and weighed after administration. The testes obtained on day 8 were weighed and stained with BODIPY and TUNEL kit. Using total RNA extracted from the isolated Leydig cells, adipogenesis and apoptosis-related genes were analyzed by real-time PCR. The testicular weights of the rats treated with 10 ㎎/㎏ TBT were significantly decreased compared to those in the control rats treated with sesame oil. As a result of BODIPY staining, the number of Leydig cells stained with BODIPY was increased in the rats treated with 10 ㎎/㎏ TBT compared with the control rats. Similar to BODIPY staining results, the TUNEL assay showed that the apoptosis of Leydig cells was increased in TBT treated rats. The results of the gene expression analysis in the Leydig cells showed that the expression of adipogenesis-related genes (PPARγ, aP2, Perilipin, CD36) and apoptosis-related genes (TNFRSF1A, TNFSF10) was increased after TBT administration. The present study demonstrates that TBT induces the expression of adipogenesis-related and apoptosis-related genes in the Leydig cells leading to adipogenesis and apoptosis in the testes. These results suggest that the dysfunction of Leydig cells by TBT exposure may cause a loss in testicular function.
송연화,최우혁 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.0
Flexible and super-stretchable Gel Polymer Electrolytes (GPEs) are widely envisioned to be promising materials for the next-generation flexible or stretchable energy storage systems such as lithium ion batteries and supercapacitors. However, the GPEs have natural limitation such as poor mechanical property for structural applications. Here, the Lithium Polyacrylate (PAALi)-based GPE hydrogels are crosslinked with vinyl silica nanoparticles (VSNP), using Di-water as medium, by one step thermal curing process in nitrogen atmosphere. Lithium acrylate monomers are selected to maximize ionic conductivity, and VSNP can provide mechanical property to stand stress during stretching. We investigated the role of VSNP on ionic conductivity, mechanical property, and morphology using dielectric spectroscopy, universal testing machine, and field-emission electron microscopy, respectively.
Aggregation of Human Eyelid Adipose-derived Stem Cells by Human Body Fluids
송연화,Sujin Yun,Jin Yang,A Young Yoon,김해권 한국발생생물학회 2012 발생과 생식 Vol.16 No.4
Fetal bovine serum (FBS) is the most frequently used serum for the cultivation of mammalian cells. However,since animal-derived materials might not be appropriate due to safety issues, allogeneic human serum (HS) has been used to replace FBS, particularly for the culture of human cells. While there has been a debate about the advantages of HS, its precise effect on human adult stem cells have not been clarified. The present study aimed to investigate the effect of HS on the human eyelid adipose stem cells (HEACs) in vitro. When HEACs were cultivated in a medium containing 10% HS, many cells moved into several spots and aggregated there. The phenomenon was observed as early as 9 days following 10% HS treatment, and 12 days following 5% HS plus 5% FBS treatment. However, the aggregation was never observed when the same cells were cultivated with 10% FBS or bovine serum albumin. To examine whether cell density might affect the aggregation, cells were seeded with different densities on 12-well dish. Until the beginning of aggregation,cells seeded at low densities exhibited the longest culture period of 16 days whereas cells seeded at high densities showed the shortest period of 9 days to form aggregation. The number of cells was 15.1±0.2×104 as the least for the low density group, and 29.3±2.8×104 as the greatest for the high density group. When human cord blood serum or normal bovine serum was examined for the same effect on HEACs, interestingly, cord blood serum induced the aggregation of cells whereas bovine serum treatment has never induced. When cells were cultivated with 10% HS for 9 days, they were obtained and analyzed by RT-PCR. Compared to FBS-cultivated HEACs, HS-cultivated HEACs did not express VIM, and less expressed GATA4, PALLD. On the other hand, HS-cultivated HEACs expressed MAP2 more than FBS-cultivated HEACs. In conclusion, human adult stem cells could move and form aggregates by the treatment with human body fluids.
송연화,최우혁 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.0
For next-generation energy storage devices, mechanical robust solid polymer electrolytes (SPEs) have attracted widespread attention due to their good processability, low cost, and dimensional stability. However, they are generally appeared a trade-off characteristic between mechanical property and ionic conductivity. Herein, the cross-linked epoxy-based SPEs containing Li salt (LiTFSI) with glymes (G3 or G4) and Ionic liquid (IL), using the ring-opening polymerization. The glymes are chosen to dissociate the Li ion from LiTFSI salt, and the IL is selected to enhance ionic conductivity. Furthermore, the epoxy resin can provide high mechanical and thermal properties. The effect of ionic liquid types on mechanical and thermal properties, ionic conductivity, and morphology characterized by using DMA, DSC, TGA, dielectric spectroscopy, and Fe-SEM, respectively.