Algal Succession on Different Substrata Covering the Artificial Iron Reef atIkata in Shikoku, Japan

최창근,손철현,Choi, Chang-Geun,Ohno, Masao,Sohn, Chul-Hyun The Korean Society of Phycology 2006 ALGAE Vol.21 No.3

Succession of artificial seaweed communities in an artificial iron reef at Ikata, southern Japan was studied based on monthly or bimonthly observations from February 1999 to August 2000. Communities were associated with different substrata (40 cm x 60 cm: steel, concrete, wood and stone) as the cover on artificial iron reefs (4.5 m x 4.1 m x 2.5 m, 45.38 m3 and 3.2 ton), which were placed on a sandy substratum at 8 m depth. Within one month diatoms dominated on all substrata with cover of approximately 100%. Enteromorpha intestinalis and Colpomenia sinuosa dominated on the reef within three months after the placement in the spring. Seaweed communities on the reef decreased during the summer. In the winter, the seaweeds on the reef recovered. Sargassum spp., Ecklonia kurome and Padina arborescens dominated on each substratum after one year. Seaweed communities on the artificial reef were similar to those on the rocky substratum around the artificial reef and also similar on different substrata covering the iron artificial reef. These results indicate that seaweed succession was impacted by season and the recruitment of spores and propagules from mature algae around the artificial reefs.

Genetic Improvement for the Low Salinity-Tolerant Porphyra Sp. by Cell Culture Technique I. Tissue Culture of Porphyra yezoensis foma narawaensis

홍용기,손철현,장정원,Hong Yang Ki,Sohn Chul Hyun,Chang Jung Won 한국양식학회 1989 韓國養殖學會誌 Vol.2 No.1

Axenic tissue culture of a marine red algae Porphyra yezoensis foma narawaensis was established for the vegetative propagation of tissues as a seed stock and for the development of a low salinity-tolerant cell line. Callus tissues have been induced from the vegetative area of blade away from the hold fast when grown on PES-agar medium. The brownish red fragile callus was maintained under fluorescent light of ca. 2000 lux with 12 : 12 hr L : D at $16^{\circ}C$. Amounts of carbohydrate and protein was determined against the weight of callus. Optimum temperature of the callus growth was $14^{\circ}C\~18^{\circ}C$. Optimun concentration of sodium chloride was $2.0\%$ for the callus growth in PES-agar medium. 양식 해조류인 큰방사무늬김에 대한 조직배양을 종자 세포 보존 및 장차 세포수준의 내저 염성 품종개량을 목적으로 시도하였다. 김 엽체의 영양 성장부위를 무균적으로 PES-한천배지 상에 배양함으로서 callus의 형성을 유도하였으며 이 적갈색의 연약한 callus를 $16^{\circ}C$에서 12시간 주기로 2000 lux의 형광등 하에서 1개월마다 이식하면서 배양하였다. 총 탄수화물 및 단백질 함량을 측정하였으며, 최적 배양온도는 $14^{\circ}C\~18^{\circ}C$였다. PES-한천배지 상에서 $2.0\%$의 NaCl 농도가 callus의 성장에 가장 우수하였다.

동해안 울진 연안 조하대 저서 해조류의 군집구조

최창근,곽석남,손철현,Choi, Chang-Geun,Kwak, Seok-Nam,Sohn, Chul-Hyun 한국조류학회(藻類) 2006 ALGAE Vol.21 No.4

Studies have been made of the species composition and variation of benthic marine algae at subtidal zone of Uljin on the east coast of Korea. Destructive method was employed to measure biomass over four seasons (2000-2002). Of 87 marine algae species identified, 11 were Chlorophyta, 29 were Phaeophyta and 47 were Rhodophyta. Dominant species in biomass were Ulva pertusa, Undaria pinnatifida and Sargassum piluliferum in summer, Laminaria religiosa, Sargassum fulvellum and Gigartina tenella in autumn, Codium fragile, Undaria pinnatifida, Sargassum horneri, S. piluliferum and S. ringgoldianum in winter, and Undaria pinnatifida, Dictyopteris divaricata, Sargassum confusum and S. horneri in spring. In general, green algae (Ulva pertusa, Codium fragile) and brown algae (Undaria pinnatifida, Sargassum fulvellum, S. horneri, S. piluliferum) appeared predominantly in the 3, 6 m depths and red algae (Gelidium amansii, Plocamium telfairiae) in the 9, 12 m depths. The barren ground of the rocky shore might provide the decrease of benthic marine algae biomass and species.

RAPD 기법을 이용한 갈파래목 해조류의 유전 변이 분석

조용철(Yong Chul Cho),박지원(Ji Won Park),진형주(Hyung Joo Jin),남보혜(Bo Hye Nam),손철현(Chul Hyun Sohn),홍용기(Yong Ki Hong) 한국수산과학회 1997 한국수산과학회지 Vol.30 No.3

The random amplified polymorphic DNAs (RAPD) technique was used to characterize seven isolates of the green seaweed Ulvales collected from Songjeng, Haeundae, Jumunjin, Dadaepo and Wando in Korea. Total DNA was extracted by the LiCI extraction method from thalli of green seaweed. The extracted DNA (3 ng) in 25 ㎕ reaction volume was amplified by 45 cycles of the polymerase chain reaction with arbitrary primers. Thirty-four primers resulted in 1227 PCR products ranged 240 bp to 1.5 kb of both conserved and polymorphic bands. Genetic similarities of the seven isolates calculated by Jaccard`s equation were ranged from 7% to 36%. Monostroma nitidum (Wando) was shown to be most distantly related with the others based on genetic similarity and did not produce the amplified band of 630 bp, common in Ulvales using primer OPB-01 (CATCCCCCTG).

同一養殖場에서 成長시킨 미역의 品種間 形態的特性과 養殖效果

이금열(Kum Yeol LEE),손철현(Chul Hyun SOHN) 한국수산과학회 양식분과 1993 韓國養殖學會誌 Vol.6 No.2

양식 톳 표복지의 재활용에 관한 연구

황은경(Eun Kyoung Hwang),조용철(Yong Chul Cho),손철현(Chul Hyun Sohn) 한국수산과학회 1999 한국수산과학회지 Vol.32 No.1

In the previous Hizikia cultivation, holdfasts were threw into the sea after harvesting in May; the young thalli (5∼10 ㎝ in length) of Hizikia are annually collected from natural bed by seed collectors for the cultivation, resulting in a ruined natural populations. Therefore, the reuse method of holdfasts by regeneration capability of Hizikia fusiformis, was investigated. The effects of emergence on the growth of regenerated thalli from holdfasts over 6 months of outdoor culture from May to November, 1995. The vegetative growth from the holdfasts was good under the emergence of 3 hrs/day on the air than 0, 1 and 2 hr/day. The regeneration of holdfast was determined by measuring total length, number of stipe and weight. The growth was facilitated under the exposure condition of 1∼3 hrs/day on the air. Outdoor cultivation for the compadson of to artificial natural seeds were conducted from December 1995 to May 1996. There was no significant differences (0.05<P) between the two kinds of seeds. Therefore, artificial seed maybe used as a replacement for the natural seed in Hizikia cultivation. From the results, an useful method was established to obtain young fronds for the cultivation using the reuse method of holdfast, to conserve the natural population of Hizikia.

청각 , Codium fragile ( Suringar ) Hariot 의 포양의 형태 및 성숙시기에 관하여

김남길(Nam Gil Kim),원용인(Yong In Won),손철현(Chul Hyun Sohn) 경상대학교 해양산업연구소 1990 수산과학연구소보고 Vol.2 No.-

홍조류 무절석회조 , 납작돌잎 ( Lithophyllum yessoense ) 사분포자체의 포자방출과 생장

황은경(Eun Kyoung Hwang),김은진(Eun Jin Kim),김형근(Hyung Geun Kim),손철현(Chul Hyun Sohn) 한국수산과학회 2002 한국수산과학회지 Vol.35 No.3

부산의 기장군 지역 조하대에 폭넓게 분포하는 무절석회조류의 한 종인 납작돌잎 (Litbophyllum yessoense)을 대상으로 하여 사분포자의 배양환경에 따른 생장과 사분포자 방출량을 정량화 함으로써 포자방출 양상을 구명하고자 하였다. 포자의 배양실험은 2000년 3월에 채집한 납작돌잎 사분포자체로부터 사분포자를 받아 수행하였다. 배양조건은 온도는 5조건 (5, 10, 15, 20, 25℃), 광량은 4조건 (0, 20, 50, 100 μmol·m^(-2)·s^(-1))과 염분농도는 6조건 (0, 9, 11, 17, 34, 43 ppt) 하에서 각각 포자의 상대생장률을 측정하였다. 월별 포자방출의 정량화는 2000년 8월부터 2001년 7월까지 매월 시료를 채집하여 50μmol·m^(-2)·s^(-1), 15℃과 16:8h (L:D) 조건하에서 48시간 동안 방출된 포자의 총 수를 계수하였다. 배우체의 최적 생장 조건은 20℃, 20 μmol·m^(-2)·s^(-1)과 34ppt조건이었으며, 최대 상대생장률은 20℃ 조건에서 0.1232였다. 포자의 방출량은 9월에 사분포자체 단위면적 (㎠) 당 266개로 최고치를 보였고, 연중 1월부터 3월까지의 시기를 제외한 모든 시기에 포자가 방출되는 것으로 나타났다. Growth and tetraspore release pattern of Litbophyllum yessoense (Rhodophyta, Corallinaceae) were investigated from March 2000 to July 2001. Pinkish tetraspores were 40.2 ± 0.4 ㎛ in diameter. After release, tetraspores attached on substrate shortly. Culture conditions were five temperatures (5, 10, 15, 20, 25℃), four irradiances (0, 20, 50, 100 μmol·m^(-2)·s^(-1)) and six salinities (0, 9, 17, 25, 34, 43ppt). Maximum growth of gametophyte was occurred at 20℃, 20μmol·m^(-2)·s^(-1), 16:8h (L:D) and 34ppt. Maximum relative growth rate was 0.1232 at 20℃. The amount of tetraspore release showed maximum at September as 266 cells per crust area (㎠), and tetraspores did not release from January to March.

톳(Hizikia fusiformis)의 초기생장에 대한 배양조건

황은경(Eun Kyoung Hwang),박찬선(Chan Sun Park),손철현(Chul Hyun Sohn) 한국수산과학회 양식분과 1997 韓國養殖學會誌 Vol.10 No.2

순간접착제를 이용한 곰피 (Ecklonia stolonifera Okamura) 의 이식효과

최창근 ( Chang Geun Choi ),김형근 ( Hyung Geun Kim ),손철현 ( Chul Hyun Sohn ) 한국수산과학회 2002 한국수산과학회지 Vol.35 No.6

순간접착제를 이용하여 곰피를 인공 기질에 부착시킨 후, 수중에 이식하여 인공 해중림 조성의 방안을 마련하기 위한 연구를 실시하였다. 계절별로 채집 동정된 해조류는 녹조류 8종 (16.7%), 갈조류 12종 (25.0%) 및 홍조류 28종 (58.3%)으로 총 48종이었다. 곰피는 2000년 11월 순간접착제로 건축용 타일 (10×10 cm)에 부착한 후, 이 타일을 수중 자연암반에 고정시켜 이식실험을 실시하였다. 이식 1개월 후 타일은 성공적으로 암반에 고정되어 75.0%의 부착률을 보였다. 타일에는 66.7%의 높은 착생율로 22개체의 곰피가 착생되어 생장하였다. 2개월 후, 타일에 착생한 곰피는 유실된 개체로 인해 6개체가 기질에 착생하여 생장하였지만, 착생초기에 비해 새로운 포복지가 많이 재생되었으며 엽체의 생육상태도 좋았다. 이식 7개월 후인 2001년 8월, 6개체였던 곰피는 포복지가 재생되어 총 71개체로 증가하였다. 곰피는 초기 이식한 개체수가 파도에 의한 유실이나 섭식에 의한 탈락이 많았지만, 건강하게 자란 개체들의 활발한 포복지 재생에 의해서 많은 개체수로 증가하였다. 이는 낮은 착생율을 보일지라도 건강하게 자란 엽체가 있을 경우, 이들 개체로부터 많은 재생이 일어날 수 있음을 시사하였다. Experimental transplantation of Ecklonia stolonifera to natural rock was carried out using adhesive glue. A plant was attached to an architecture tile (10×10 cm) with the glue, and then the tile was attached to natural rock with underwater glue. Within one month, the tile were fixed to natural rock with an attachment rate of 75%, and E. stolonifea grew up with an insertion rate of 66.7% on the tile. After two months, number of E. stolonifera on the tile decreased. However, the attached plants were regenerated by new stolons, maintaining good growing condition. After seven months, E. stolonifera increased 6 to 71 individuals. These results indicate that regeneration of plants was mainly affected by the recruitment of stolon around the plant.