소아에서 IgE 매개성과 비 IgE 매개성 아토피피부염의 특징

김형수 ( Hyung Su Kim ),정지인 ( Ji In Jung ),서선복 ( Sun Bok Suh ),정진아 ( Jin A Jung ) 대한소아알레르기호흡기학회 1991 소아알레르기 및 호흡기학회지 Vol.1 No.4

Purpose: Atopic dermatitis (AD) is a chronic inflammatory skin disorder with a association of genetic, environmental, and immunologic factors in the development of AD. And AD can be classified into IgE mediated and non-IgE mediated. We investigated a difference of clinical characteristics and immune response between the two groups. Methods: From January 2008 to December 2011, we enrolled 125 children who visited Dong-A University Medical Center and Busan Medical Center, and were diagnosed as AD with the Haniffin and Rajka`s criteria. We checked the history of combined asthma and allergic rhinitis and allergic disease of family in patients. We measured serum total IgE, specific IgE by ImmunoCAP or skin prick test. We measured serum interleukin (IL) 4 (IL-4), interferon-γ (IFN-γ), IL-10, and IL-17, which are associated with chronic inflammatory disorder by flow cytometry method (Luminex). Results: Eighty (64%) were included in the IgE mediated group, while forty-five (36%) were included in the non-IgE mediated group. The frequency of combined allergic disorder and serum total eosinophil count were relatively higher in IgE mediated group (P=0.023, P=0.032). The incidence of a family history in allergic disease and the mean of SCORing Atopic Dermatitis index had no difference between the two groups. Serum IL-4, IFN-γ, IL-10, IL-17 were higher in the IgE mediated group, but there were no statistically significant differences between two groups (P>0.05). Conclusion: IgE mediated AD showed higher total eosinophil count and higher incidence of bronchial asthma and allergic rhinitis than non IgE mediated AD. (Allergy Asthma Respir Dis 2013;1:339-343)

소아에서 IgE 매개성과 비 IgE 매개성 아토피피부염의 특징

김형수 ( Hyung Su Kim ),정지인 ( Ji In Jung ),서선복 ( Sun Bok Suh ),정진아 ( Jin A Jung ) 대한천식알레르기학회 2013 Allergy Asthma & Respiratory Disease Vol.1 No.4

Purpose: Atopic dermatitis (AD) is a chronic inflammatory skin disorder with a association of genetic, environmental, and immunologic factors in the development of AD. And AD can be classified into IgE mediated and non-IgE mediated. We investigated a difference of clinical characteristics and immune response between the two groups. Methods: From January 2008 to December 2011, we enrolled 125 children who visited Dong-A University Medical Center and Busan Medical Center, and were diagnosed as AD with the Haniffin and Rajka`s criteria. We checked the history of combined asthma and allergic rhinitis and allergic disease of family in patients. We measured serum total IgE, specific IgE by ImmunoCAP or skin prick test. We measured serum interleukin (IL) 4 (IL-4), interferon-γ (IFN-γ), IL-10, and IL-17, which are associated with chronic inflammatory disorder by flow cytometry method (Luminex). Results: Eighty (64%) were included in the IgE mediated group, while forty-five (36%) were included in the non-IgE mediated group. The frequency of combined allergic disorder and serum total eosinophil count were relatively higher in IgE mediated group (P=0.023, P=0.032). The incidence of a family history in allergic disease and the mean of SCORing Atopic Dermatitis index had no difference between the two groups. Serum IL-4, IFN-γ, IL-10, IL-17 were higher in the IgE mediated group, but there were no statistically significant differences between two groups (P>0.05). Conclusion: IgE mediated AD showed higher total eosinophil count and higher incidence of bronchial asthma and allergic rhinitis than non IgE mediated AD.

곁사슬형 Polyethylene Glycol을 가진 Adenosine Deaminase 거대분자 결합체의 생화학적 특성

서선복,이순용 인제대학교 1995 仁濟醫學 Vol.16 No.4

혈색소, 홀몬 및 효소들과 같은 단백질은 질병 치료에 흔히 이용되고 있으나 이들은 생체 내로 투여하였을 때 치료제로서의 안정성과 항원성이 문제가 되어 그 효능을 기대하기 힘든 경우가 적지 않다. 저자들은 이러한 결점을 개선해 보고자 그 첫 단계로 비항원성 합성 고분자 화합물인 곁사슬형 polyethylene glycol(PEG)을 단백질의 실험모델로 사용한 효소 adenosine deaminase(ADA)에 결합시켜 이 복합체(PEG-ADA)의 물리적 및 생화학적 성질에 대하여 조사하였다. 연구 결과 곁 사슬형 PEG-ADA는 PEG로 인해 ADA가 변형되었지만, 효소활성이 유지되고 있었으며 단백질 분해효소에 대해서도 안정되어 있음을 보여 주었다. The enzyme is usually limited to use as a therpeutics because of its own instability and antigenicity. Adenosine deaminase(ADA), which was selected as a model of enzymes in this study, was modified by a pendant-type polyethylene glycol(PEG) in order to reduce the nzyme's own disadvantages, and the authors studied about the biochemical characteristics of the pendant type PEG-ADA conjugate to elucidate whether this conjugate could be a more improved and effective therapeutic enzyme haying stability and non-antigenicity. Calf adenosine deaminase was modified with pendant-type polyethylene glycol using cyanuric chloride as a coupler. The covalent attachmant of PEG to ADA altered the kinetic profile of the enzyme in which the value of Km for adenosine was decreased. The Km value of the ADA and PEG-ADA10 were 30 μM, 25 μM against adenosine, respectively. But the PEG-ADA20 exhibited reasonable retention of catvlytic activity with an increased Km, which was 50 μM against adenosine. The ADA and PEG-ADA showed optimum in acidic direction. The ADA and PEG-ADA had same temperture optima of 50℃. Thermal inactivation at the temperature above optima was essentially the same between the native and modified enzymes. PEG appeared to have no effect of stabilizing enzymes on high temperature. This suggested that PEG attachment caused some destabilization of the adenosine deaminase structure. PEG-ADA also demonstrated a resistance to the proteolytic digestion by trypsin and chymotrypsin. The electrophoretic mobility in polyacrylamide gels of PEG -ADA was reduced as compared to the native enzyme. The unmodified enzymes showed single band, whereas the modified ones formed a mumber of bands throughout the gels. The decreased electrophoretic mobility of PEG-ADA might be due to a charge-shielding effect of the hydrophilic PEG shell surrounding the enzyme. In addition, the increased size of the adducts might serve to retard migration in the polyacrylmide gels. The alteration of the pH dependance and the dramatically decreased mobility in polyacrylamide gel electrophoresis of the PEG-enzyme pointed up the effect of PEG modification on the apparent charge of the enzyme molecules. It was believed to be due to a change in conformation dictated by the charge of the PEG-enzyme or imposed by the covalent attachment of PEG to the enzyme. These results showed that the biochemical properties of adenosine deaminase were altered after chemical modification by pendant type PEG. The preparation of PEG-ADA retained the enzyme activity and had resistance against proteolytic enzymes. The authors concluded that the pendant-type PEG-ADA might be available in the medical field in the future.

중격류가 형성된 심실중격결손의 임상 및 혈역학적 특성

김철호,서선복 인제대학교 1988 仁濟醫學 Vol.9 No.3

심실중격결손의 자연폐쇄기전 중 중요한 부분을 차지하는 것으로 알려진 중격류 형성에 대한 임상 및 혈역학적 특성에 대하여 분석 관찰하여 보았다. Twenty five cases of VSD with septal aneurysm which was confirmed by LV angiography and 2-D echocardiography during the period of 18 months from November 1986 to April 1988 at Pusan Paik Hospital were studied and analyzed. The summary of studies are as follows : 1) Septal aneurysm was found in 25 (36%) of 69 cases of total VSD and in 45% among membranous VSD. 2) In analyzing 25 cases with septal aneurysm, the sex ratio was not different significantly and age group of 1-5yrs was involved more predominantly than other age groups. The youngest patient was 9 month-old male. 3) Among 25 cases with septal aneurysm, 3 cases (12%) had previous heart failure. 4) In almost 80% of the patients with septal aneurysm, EKG and chest X-ray findings were non-specific or nearly normal. 5) Qp/Qs in cases with septal aneurysm was below 2.0 in 95% of the cases, and was below 1.5 in 75% of the cases. Right ventricular pressure was also within normal limits in most of cases. 6) There was no specific problems complicated by septal aneurysm. Partial obstruction of right ventricular outflow tract was observed in 1 case. 7) There was no significant difference in detection rate between 2-D echocardiography and LV angiography; which was 80%, 88% respectively.