Aspergillus flavus 에 의한 Aflatoxin 생산능에 관한 연구 - 균류 , 버섯 , 아미노산 및 비타민이 미치는 영향 -

서명자(Myung Ja Suh) 경희대학교 동서약학연구소 1981 경희약대 논문집 Vol.9 No.-

N/A

韓國産 Comfrey의 食品學的 價値에 關한 硏究

서명자(Myung-Ja Suh) 한국식품영양과학회 1978 한국식품영양과학회지 Vol.7 No.2

General components of comfrey collected during the period of July-September 1978 in Pusan area were investigated with a view to determine its possible value as a foodstuff by means of titrimetry, chromometric analysis and fluorometric analysis. As a result, the following data were obtained.<br/> 1) The contents of moisture, crude lipid, crude protein and carbohydrates of the comfrey were similar to those of other green vegetables such as spinach, crowndaisy, lettuce, leek and mallow.<br/> 2) The inorganic Ca, P and Fe of the comfrey were fairly greater in contents than those of other green vegetables. Compared with spinach, for instance, there were 4 times as much Ca and about 2 times as much Fe in it, respectively.<br/> 3) The contents of vitamins of the comfrey were also found in an amount much greater than those of other green vegetables. V-A of the comfrey, for instance, were 6 times greater in content than that of lettuce, and about 2 times greater than that of crowndaisy, leek and mallow, respectively. It also showed a greater content of V-A than even that of spinach, known as a vegetable most rich in V-A.<br/> V-B of the comfrey showed a little lower level of content than that of spinach but showed about the same level of content as that of leek and lettuce. V-B of the comfrey was 6 to 8 times greater in content than that of lettuce and leek. The content of niacin of the comfrey was also found much greater than those of other foodttuffs. V-C of the comfrey compared 3.5 times greater with that of lettuce, 2 times greater with that of mallow, and generally a higher level with those of other green vegetables.<br/> The above findings seem to firmly establish the food scientific value of the comfrey. Furthermore, the vegetable is very prolific and grows easily. If the general public is made aware of these facts, the vegetable is thought to contribute not a little to their dietary life as a nutritious foodstuff.

Purification and Characterization of the Red Carotenoprotein from the Skin of Ascidian, Halocynthia roretzi 2. Chemical Structure of Carotenoid

강옥주,서명자,이안종,김세권,Kang, Ok-Ju,Suh, Myung-Ja,Lee, An-Jong,Kim, Se-Kwon Korean Society of Life Science 1995 생명과학회지 Vol.5 No.4

Carotenoids liberated from the red carotenoprotein from the skin of ascidian(Halocynthia roretzi)were developed on TLC, and two bands were obtained with different Rfvalues. Their chemical reactivities and spectroscopic properties were studied and elucidated as astaxanthin diester and astaxanthin monoester. After saponification of carotenoid ester, GC analysis was performed. The carotenoid ester contained oleic acid, palmitic acid and palmitoleic acids as 50% of total amount, and had higher content unsaturated fatty acid including eicosapentanoic acid than saturated fatty acid.

Purification and Characterization of the Red Carotenoprotein from the Skin of Ascidian, Halocynthia roretzi 1. Purification and Characterization of the Caritenopritein

강옥주,서명자,이안종,김세권,Kang, Ok-Ju,Suh, Myung-Ja,Lee, An-Jong,Kim, Se-Kwon Korean Society of Life Science 1995 생명과학회지 Vol.5 No.4

A carotennnoprotein from the skin of Ascidian(Halocynthia roretzi) was extracted by Triton X-100 and purified by ammonium sulfate fraction, SephadexG-200 charomatography and DEAE-cellulose ion exchange chromatography. The carotenoprotein was redwith broad $\lambda$$_{max}$ between 495, 467 and 318nm. The red carotenoprotein had an approximate molecular weight of 326KDa(gel filtration). SDS-PAGE indicated the presence of two polypeptodes of 84.1KDa and 74.4KDa, with different mobility in polyacrylamide gel electrophoresis. In the presence of denaturing agents such as organic solvent aand extreme pH, the red complex readily disociates to liberate the yellow carotenoid($\lambda$$_{max}$ 452nm) and a colourless apoprotein. The amino acid composition of carotenoprotein were mainly threonine(15.2%), aspartic acid(12.2%), glutamic acid(11.9%) and serine(9.6%), while proline was not found. The carotenoprotein consisted of lipids as structure units. Its major fatty acids composion were C$_{18:1}$, C$_{16:1}$, and C$_{16:0}$. The monounsaturated fatty acids(41.5%) contained abundant content compared to other fatty aacids(polyunsaturated fatty acids 37.4%, saturated fatty acids 20.6%).

1,25(OH)₂-23ene-D₃ : in vitro에서 U937 세포의 증식과 분화 및 in vivo에서 쥐의 칼슘대사에 미치는 영향

정수자(Soo-Ja Jung),서명자(Myung-Ja Suh) 한국식품영양과학회 1995 한국식품영양과학회지 Vol.24 No.1

1,25(OH)₂-23ene-D₃는 23번과 24번 탄소 사이에 이중결합을 가진 새로운 비타민 D₃ 유도체로서 이 화합물의 항악성 효과를 검토하기 위하여 in vitro에서 U937 세포(human histiocytic lymphoma cell line)의 증식과 분화에 대한 효과 및 in vivo에서 쥐의 칼슘대사에 미치는 영향을 관찰하였다. 본 연구에서 1,25(OH)₂-23ene-D₃의 효과는 비타민 D₃의 자연 대사물인 1α, 25-dihy-droxycholecalciferol [1,25(OH)₂D₃]과 비교 검토하였다. 1,25(OH)₂-3ene-D₃는 1,25(OH)₂D₃에 비해 U937 세포 증식억제 및 분화촉진에 강한 효과가 있음이 확인되었고, 특히 superoxide 생성과 nonspecific esterase (NSE) 활성으로 측정한 세포의 분화촉진 효과는 1,25(OH)₂D₃에 비해 20배 이상 높은 것으로 나타났다. 또한 1,25(OH)₂-23ene-D₃로 처리된 U937 세포는 N/C비 감소, 배양말기에 부착세포수 증가 등의 현상을 나타내면서 분화에 따른 세포형태 변화를 더욱 뚜렷히 보였다. 쥐에 이들 비타만 D₃ 유도체를 투여한 결과 1,25(OH)₂-23ene-D₃는 hypercalcemia와 hypercalciuria를 야기시키는 활성이 1,25(OH)₂D₃에 비해 50배 이상 낮음이 확인되었다. 1,25(OH)₂-23ene-D₃가 칼슘대사에 미치는 활성이 낮으면서 U937 세포의 증식과 분화에 직접적인 강한 효과를 가지고 있음이 밝혀짐에 따라 앞으로 백혈병 환자나 건선(psoriasis)등의 피부성 질환을 가진 환자의 임상연구에 이용이 가능한 화합물이라 생각된다. 1,25(OH)₂-23ene-D₃ is a novel vitamin D₃ analog which has a double bond between C-23 and C-24. We describe the effects of this analog on cell differentiation and cell proliferation in vitro using the human histiocytic lymphoma cell line U937, and on calcium metabolism in rats in vivo. In the present investigation 1,25(OH)₂-23ene-D₃ was compared to the natural metabolite of vitamin D₃, 1α, 25-dihydroxycholecalciferol [1,25(OH)₂-D₃]. 1,25(OH)₂-23ene-D₃ was more potent than 1,25(OH)₂D₃ for inhibition of proliferation and induction of differentiation of U937 cells. Especially, its effect on induction of differentiation, as measured by superoxide production and nonspecific esterase(NSE) activity, was about 20-fold more potent than 1,25(OH)₂D₃. This analog morphologically and functionally differentiated U937 cells to monocyte-macrophage phenotype showing a decrease of N/C ratio in Giemsa staining and the increase of adherence ability to surface. Intraperitoneal administration of 1,25(OH)₂-23ene-D₃ to rats showed that the compound had at least 50 times less activity than 1,25(OH)₂D₃ in causing hypercalcemia and hypercalciuria. The strong direct effects of 1,25(OH)₂-23ene-D₃ on cell proliferation and cell differentiation, coupled with its decreased activity of calcium metabolism make this compound an interesting candidate for clinical studies including patients with leukemia, as well as several skin disorders, such as psoriasis.

Effects of a New 1,25(OH)₂ - Vitamin D₃ Analog on Proliferation and Differentiation of the Human Histiocytic Lymphoma Cell Line U937

정수자(Soo-Ja Jung),서명자(Myung-Ja Suh),류병호(Beung-Ho Rhu) 한국식품영양과학회 1994 한국식품영양과학회지 Vol.23 No.3

인체 histiocytic lymphoma cell line U937의 증식억제 및 분화촉진 효과에 대한 새로운 vitamin D₃ 유도체인 1,25(OH)₂-16ene-23yne-26,27-F^6-D₃의 영향을 관찰하였다. 10^(-7)M 및 10^(-8)M 농도에서 이 화합물은 U937 cells의 증식을 각각 87% 및 62%까지 억제시켰다. 분화촉진 효과에 관한 연구에서는 세포의 morphology 변화와 세포분화의 marker로써 이용되는 nonspecific esterase(NSE) activity 및 배양기 세포의 adherence abilty를 검토한 결과 이화합물은 U937 cells을 macrophage으로 분화시킴이 확인되었다. 또한 10^(-7)M 농도에서 이화합물은 U937 cells의 c-myc mRNA expression을 60% 정도 감소시킴이 확언되어 1,25(OH)₂-16ene-23yne-26,27-F^6-D₃는 U937 cells에 대해 분화를 유도하는 factor임을 본 연구 결과 알 수 있다. This study describes the effects of a novel 1,25-dihydroxyvitamin D₃ analog [1,25(OH)₂-16ene-23yne-26,27-F_(6)-D₃] on proliferation and differentiation of the human histiocytic lymphoma cell line U937 in vitro. We also examined the expression of c-myc oncogene in U937 cells. Growth of U937 cells was apparently inhibited to 62% and 87% of the control level after 4 days in the presence of 10^(-8)M and 10^(-7)M of this analog, respectively. This compound morphologically and functionally differentiated U937 cells to monocyte-macrophage phenotype showing the increase of adherence ability to surface and a decrease of N/C ratio in Giemsa staining. Especially, nonspecific esterase activity which is a marker of cell differentiation to monocyte-macrophage was positive, and production of the positive stained cells increased in a dose dependent fashion. The expression of c-myc oncogene by 1,25(OH)₂D₃ analog(10^(-7)M) was reduced by 60% at the mRNA level as determined by Northern blotting. The effects of this novel analog on cell proliferation and cell differentiation may open up new therapeutic strategies for human disorders such as psoriasis and may provide a tool to understand the mechanism of action of vitamin D₃ seco-steroids in malignancy.

수은이 Lactobacillus bulgaricus의 젖산 생성과 생육 저해작용에 대한 양파 추출물의 효과

김소희(So Hee Kim),서명자(Myung Ja Suh) 한국생약학회 1987 생약학회지 Vol.18 No.1

Onion extracts were tested for effects on the growth and lactic acid production of Lactobacillus bulgaricus in the medium containing mercury. The media containing mercury were added with onion extracts and inoculated with the bacterium and then incubated at 39 for four days. All of the onion extracts examined increased the growth and lactic acid production of the bacterium in the medium containing mercury. At the addition of the edible portion extract of onion to the medium containing 5 ppm, 10 ppm and 20 ppm mercury, the higher the concentration of the onion extract was added, the greater the increasing effect on the growth and lactic acid production. The brown peel extract of onion increased the growth and lactic acid production of the bacterium in the medium containing mercury at all concentrations. The higher the concentration of the extract added, the more effective the increasing effect. The onion solutions of edible portion and brown peel extracted at 90 to 100 showed more desirable effects than those extracted at the room temperature. Among four kinds of the onion extracts, the brown peel solution extracted at 90 to 100 was the most effective in increase of the growth and lactic acid production in the medium containing mercury.

Sodium Alginate와 Cellulose가 흰쥐의 공복 혈장 Lipoprotein 조성과 콜레스테롤 대사에 미치는 영향(Ⅰ)

강희정(Hee-Jung Kang),서명자(Myung-Ja Suh),김은희(Eun-Hee Kim),송영선(Young-Sun Song) 한국식품영양과학회 1994 한국식품영양과학회지 Vol.23 No.6

Sodium alginate와 cellulose가 흰쥐의 혈청 지단백 조성과 지질대사에 미치는 영향을 조사하였다. 각 식이섬유를 10% 수준으로 첨가한 식이로 흰쥐를 4주간 사육한 후, 혈장 지단백 조성, 간 지질 조성, microsomal HMG-CoA reductase 활성과 분변 중의 지질 조성 및 담즙산 함량을 측정하였다. 식이섬유 첨가군의 혈장 콜레스테롤 농도는 무섬유식이군에 비해 다소 감소되었으나 유의적인 차이를 보이지 않았다. Sodium alginate 첨가군의 Chylomicron / VLDL과 LDL 및 간의 콜레스테롤 농도와 혈장과 Chylomicron / VLDL 및 간의 중성지방 농도는 cellulose 첨가군과 무섬유식이군에 비해 유의적으로 감소되었다(p<0.05). Sodium alginate 첨가군의 HDL 콜레스테롤 농도는 다른 식이군에 비해 다소 증가하였다. Sodium alginate 첨가군의 분변 중 콜레스테롤, 담즙산 및 중성지방의 배설량은 다른 식이군에 비해 유의적으로 증가되었으며(p<0.05), microsomal HMG-CoA reductase 활성은 식이군간에 유의적인 차이를 보이지 않았다. 이러한 결과는 sodium alginate의 섭취가 혈장과 간의 중성지방 및 콜레스테롤 농도를 낮추는 효과 (hypotriglyceridemic and hypocholesterolemic effect)가 있음을 시사한다 하겠다. This study was carried out to determine the effects of sodium alginate and cellulose on the plasma lipoprotein composition and cholesterol metabolism in rats. Each experimental diet contained 10% sodium alginate and cellulose by weight, respectively and rats were fed for 4 weeks. The results obtained were as follows: The feeding of sodium alginate and cellulose decreased total plasma cholesterol slightly. Total cholesterol of Chylomicron / VLDL-, LDL-fraction and liver were decreased significantly in sodium alginate group. HDL-cholesterol was slightly increased in sodium alginate group. The feeding of sodium alginate significantly lowered plasma, Chylomicron / VLDL-, LDL-fraction and liver TG concentrations compared with those fed fiber-free diet. The HMG-CoA reductase activity was not different among diet groups but the lowest activity was observed in sodium alginate group. The feeding of sodium alginate significantly increased fecal cholesterol, TG, and bile acid excretion. In summary, the ingestion of sodium alginate decreased cholesterol and TG concentrations of plasma and liver. This may be explained by the facts that fecal cholesterol, bile acid and TG level were increased significantly in sodium alginate group.

감자 lipoxygenase가 밀가루반죽의 지질분포, 지방산조성, 카로테노이드 및 색도 변화에 미치는 영향

문정원(Jung-Won Moon),서명자(Myung-Ja Suh) 한국식품과학회 1995 한국식품과학회지 Vol.27 No.3

식이섬유 식이에 적응된 흰쥐에서 고지방식이가 식후 혈장지질농도와 소화효소활성에 미치는 영향

양정례(Jeong-Lye Yang),서명자(Myung-Ja Suh),송영선(Young-Sun Song) 한국식품영양과학회 1997 한국식품영양과학회지 Vol.26 No.1

식이섬유에 5주간 적응된 흰쥐(S.D., male)를 14시간 절식시킨 뒤 총 에너지의 50%가 되는 고지방식이를 급여하고 4시간 뒤의 혈장 지질 농도와 소화효소활성을 조사하여 다음과 같은 결과를 얻었다. 혈장과 지단백의 콜레스테롤 함량은 sodium alginate군에서 가장 낮았다(p<0.05). 혈장 중성지방 함량은 식이군간에 차이가 없었으나 LDL-중성지방은 sodium alginate군에서 가장 낮았다(p<0.05). HDL-중성지방 함량은 식이섬유군에서 높았다(p<0.05). 소장 apolipoprotein B는 무섬유식이군과 cellulose군에 비해 sodium alginate군에서 유의적으로 높았다(p<0.05). 소장 내용물에서의 amylase 활성은 각 식이군간에 유의적인 차이는 없었으나 식이섬유 첨가군에서 다소 높았으며, lipase 활성은 무섬유식이군과 cellulose군에 비해 sodium alginate군에서 유의적으로 높았다(p<0.05). 소장내용물에서 protease 활성과 담즙산 함량은 식이군간에 유의적인 차이가 없었으나, 단백질 함량은 무섬유식이군에 비해 식이섬유 첨가군에서 유의적으로 높았다(p<0.05). 이상의 결과를 종합해 볼 때 장기간의 식이섬유 섭취는 식이섬유가 배제된 고지방식 후에도 혈장 콜레스테롤 농도를 낮추는 효과가 있으나, 혈장 중성지방 농도는 식이섬유가 함유된 식이에 비해 영향을 받지 않는 것으로 보인다. Rats were adapted to diets containing 10% cellulose, 10% sodium alginate and fiber-free diet for 5 weeks. Following a 14 hour fasting, rats were fed 5g of a test meal that provided 50% energy from fat, then killed at 4 hour postprandially. Plasma and lipoprotein fraction-cholesterol levels were lower in sodium alginate-fed animals than in rats fed other diets. Plasma TG did not differ among diet treatments. Increase in TG content of HDL fraction occurred in dietary fiber groups. Intestinal apolipoprotein B level and lipase activity were lower in sodium alginate-fed group than in other dietary groups. These results suggest that chronic consumption of sodium alginate affects plasma cholesterol level as in the case of fiber supplemetation, but is less likely to modify the acute plasma TG response to high fat meal than if a fiber supplement is incorporated into the meal.