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Radical Scavenging Active Compound Analysis in Extract of Coffee Silver Skin
이경인,백지환,표병식,최철희 한국약용작물학회 2023 한국약용작물학회지 Vol.31 No.4
Background: Coffee silver skin is a by-product generated during the processing of coffee, which has recently been cultivated mainly in the southern regions of Korea. Radical scavenging active compounds were analyzed as part of a study of active compounds present in coffee silver skin. Methods and Results: Hot water extracts and ethanol extracts were prepared at varying concentra- tions (10% - 95%). Simultaneously with liquid chromatography (LC) separation, 1,1-diphenyl-2- picrylhydrazyl radical scavenging activity was measured and mass spectrometry (MS) of the iden- tified radical scavenging active compound peak was performed. The analysis results confirmed the presence of three compounds, eicosanoyl-5-hydroxytryptamide, docosanoyl-5-hydroxytryptamide, and tetracosanoyl-5-hydroxytryptamide. In the rapid LC-MS/MS analysis using the multiple reac- tion monitoring mode, each active compound showed a higher content as the ethanol concentration increased. In particular, the content of each compound was found to be significantly decreased in the extract with an ethanol concentration of 60% or less. Conclusions: The three identified alkanoyl-5-hydroxytryptamide compounds are reported to pos- sess antioxidant activity and various neurological disease-related effects. Therefore, this analysis method can be applied to research or product development using coffee silver skin
Comparison of Major Compounds of Coffee Tree Leaf Extract by Varieties Cultivated in Korea
이경인,백지환,표병식,차상화 한국약용작물학회 2022 한국약용작물학회지 Vol.30 No.6
Background: To identify suitable varieties of coffee for Korea, various varieties of coffee trees are being cultivated. In relation to this process, it is necessary to develop the analysis methods to confirm and compare the characteristics of each variety. Methods and Results: In this study, ethanol extracts of coffee tree leaves of four varieties, Coffea arabica var. bourbon (Bourbon), C. arabica var. maragogype (Maragogipe), C. arabica var. typica (Typica), and C. arabica var. geisha (Geisha), were targeted. The liquid chromatography (LC) profile of each extract was analyzed using a ultra-violet detector. Sixteen major peaks were selected from the LC profile analysis results, and mass spectrometry (MS) was performed using an LC-MS/ MS system with the same separation conditions. The MS results confirmed that the major peaks were caffeoyl quinic acid, procyanidin, dicaffeoyl quinic acid, mangiferin, cinchonain I isomer, iriflophenone 3-C-glucoside, caffeine, and theobromine. The quantities of these major compounds were compared for each variety through additional multiple reaction monitoring mode MS analysis. Conclusions: Differences in the major compound contents of the leaves of different domestic coffee tree varieties were confirmed, and LC-MS/MS analysis was found to be useful for the comparative analysis of compounds between varieties.