http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
고초균으로부터 β - glucanase 유전자의 분리 및 대장균에서의 발현
서연수,이영호,백운화 ( Yeon Soo Seo,Young Ho Lee,Un Hua Pek ) 생화학분자생물학회 1985 BMB Reports Vol.18 No.4
A gene coding for β-glucanase which can specifically hydorlyze barley β-glucan and lichenan was isolated from Bacillus subtilis ATCC6633 by molecular cloning using a plasmid vector pBR325. 2.1 Kb BamHI-HindIII DNA fragment conferred β-glucanase activity on Escherichia coli HB101 and 1.7 Kb PstI DNA fragment included in 2.1 Kb fragment gave rise to β-glucanase producing colonies, while its expression depended upon its inserting orientation in pUC9. This gene directed efficiently the synthesis of β-glucanase in E. coli. Up to 2496 of total activity was found outside of the cells. Fine restriction map was also constructed for further sequencing analysis. The transcriptional direction of cloned β-glucanase gene was determined and its putative functional unit could be deducted from the molecular weight of β-glucanase and by deletion mapping.