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      • SCOPUSKCI등재
      • KCI등재

        서울지역 교육시설 및 공원 내 토양 중 분원성대장균군 오염실태

        김혜라 ( Hye Ra Kim ),김능희 ( Neung Hee Kim ),박형숙 ( Hyong Suk Park ),김두환 ( Doo Hwan Kim ),이주형 ( Ju Hyung Lee ) 한국동물위생학회 2014 韓國家畜衛生學會誌 Vol.37 No.4

        Fecal coliform bacteria are indicators of fecal contamination and of the potential presence of pathogens associated with animal excreta, wastewater, sludge. This study examined the distribution of fecal coliform in sand of the playground at educational facilities and parks in Seoul. Fecal coliform were detected from 61 (22.1%) places out of 276 playgrounds. Escherichia (E) coli (75.4%) was found most frequently, followed by Cronobacter (Cro) sakazakii (8.7%), Klebsiella (K) pneumoniae (7.2%) and Actinobacter baumannii (4.3%). We also tested the bacteria for their susceptibility to 16 antibiotics using disk diffusion method. All the isolates were highly susceptible to cefepime, gentamicin, tetracycline, ciprofloxacin, enrofloxacin, nalidixic acid and sulfamethoxazole/ trimethoprim, but resistant to apramycin and cephalothin. E. coli and Cro. sakazakii were highly resistant to apramycin (100% and 83.3%), followed by cephalothin (51.9% and 50%) and ampicillin (19.2% and 16.7%). K. pneumoniae was highly resistant to ampicillin and apramycin (100%, respectively) but the remaining antibiotics were susceptible.

      • KCI등재

        서울지역 동물보호소 내 유기견의 개 심장사상충 감염 실태조사

        김능희 ( Neung Hee Kim ),곽정연 ( Jeong Yeon Kwak ),김혜라 ( Hye Ra Kim ),박형숙 ( Hyeong Suk Park ),김두환 ( Doo Hwan Kim ),이주형 ( Ju Hyung Lee ) 한국동물위생학회 2014 韓國家畜衛生學會誌 Vol.37 No.3

        Dirofilaria immitis infection is one of the most important parasitic diseases in dogs and public health. It often elicits nodules in the pulmonary parenchyma and in the subcutaneous tissues of human. In this study, we investigated the prevalence of D. immitis infection among 754 dogs in Seoul area from April to December 2012. The infection rate of 754 dogs was 9.8% (74/754) by the antigen test (canine SNAP 4Dx). The infection rates of D. immitis in dogs at the age of <2, 2∼4, 5∼7, 8∼10, and >10 years were 3.7% (7/189), 12.9% (30/233), 15.0% (19/127), 9.8% (18/184), and 0% (0/21), respectively. The infection rates in the female and male dogs were 9.7% (33/340) and 9.9% (41/414), respectively. The regional infection rates in northern and southern Han river region of Seoul were 10.7% (44/410) and 8.7% (30/344), respectively. In order to confirm D. immitis infection, we performed PCR on serum samples of 74 dogs which tested positive for D. immitis antigen by the antigen test and we detected a specific gene ITS-2 in 59 serum samples. In conclusion, this study suggests that treatment and preventive care on the D. immitis infection should be considered in dogs of Seoul area.

      • KCI등재

        서울지역 사육 소의 큐열 및 톡소포자충 항체 보유율 조사

        김능희 ( Neung Hee Kim ),김혜라 ( Hye Ra Kim ),박형숙 ( Hyung Suk Park ),김영섭 ( Young Sub Kim ),이주형 ( Ju Hyung Lee ) 한국동물위생학회 2015 韓國家畜衛生學會誌 Vol.38 No.4

        Both Q-fever and Toxoplasmosis are zoonosis. Q-fever occurs due to intracellular bacteria, while Toxoplasmosis is created by protozoan. Both of them have a wide range of host including livestock and wild animals and occur sporadically all over in the world. In this study, seroprevalence of Q-fever and Toxoplasmosis was investigated on cows bred in the area of Seoul where there was a fairly high possibility to occur, while vaccine was not used in Korea. As for experiment materials, cattle blood collected from 190 cows from February to September in 2014 was used ELISA. According to the result, there was a positive reaction on Q-fever from 18 cows out of total 190 cows (9.5%) and on Toxoplasmosis from 32 cows (16.8%). Seroprevalence of both diseases per age was turned out to be negative for those aged less than 2. In addition, it was shown to be positive on 4 cows out of 87 (4.6%) cows aged from 3 to 5, on 7 cows out of 30 cows (23.3%) aged from 6 to 7. Finally, it was shown to be positive on 7 cows out of 17 cows (41.2%) aged 8 or above. Toxoplasmosis was turned out to be positive on 1 cow out of 56 cows (1.8%) aged less than 2, on 6 cows out of 87 cows (6.9%) aged from 3 to 5, on 17 cows out of 30 cows (56.7%) aged from 6 to 7. In addition, it was turned out to be 8 cows out of 17 cows (47.1%) aged 8 or above. Seroprevalence of both diseases was turned out to be higher as age increased. Therefore, it seems that a wide range of investigation on the entire disease spreaders as well as livestock is required since infection of Q-fever and Toxoplasmosis, types of zoonosis, has continuously occurred, and the number of insects, wild animals, and stray animals serving as a role of spreading diseases by changes in seasons and environments has been gradually increasing in Korea.

      • KCI등재

        초등학교 아동의 비만지수와 신체활동에 관한 조사연구

        신재신,김명희,박형숙,송미경 韓國學校保健學會 2000 韓國學校保健學會誌 Vol.13 No.1

        This study researches an obesity inde( and the reaiities of physical activity of etementary school childrnl and pvide basic data to instruct correct physical activity for low weight md obese childraL The subject was 813 Elementary School Chiliherl of grade 4-6 in Krimgiu and data were collected with a Physical Activir Questionnaire from fTs, USA The collected data ware an3ly2ed with real number, pnfentagf avErge and standard deviation techniques. The rarlge was by SPSS WIN. Both of them were in the normal range, between -38.3 and 77.7, between low weight and obesity The results are as follows : 1. An Obesity index according to grade and sex: the average obesity index of 423 boys was 7.56 ± 15.7, for girls it was 2.56± 14.3. Both of them were in the nrnmal range, between -38.3 and 77.7. There were low weight(-38.85±0.9) students as well as obese, including extremely low weight children (-38.85 ±0.9) and extremely obese children. 2. On the whole, in the Freouency of the 25 item physical activities breakdown, children reported to watch TV 1.(2.7±0.6), or do homework(2.1 ±0.9), more than three-fourths of the week, male children, however, played more football(2.0 ±0.9). As male obese children do less MET5, MET8(highly active activity) than male normal children, it was also discovered that low weight children do mnre severe activity in MET8(high activity). In female cases, it was revealed that neither do highly intense activity. 3. In physical activitiy, male children received higher points than fonale children with 92.94±37.95 to 74.46 ±29.50. On the whole, male and female obese children had lower score than normal children. 4. As for attitude toward physical activiv, male children(31.9614.37) had a positive attitude than female children (29.57 ±3.89), but obese male children had a negative attitude regarding activity viewed as normal. ThaefgrE in peventing obesity in elementary school childrerl the treating of under-weight childrern. should be included in the obesity Fgram so as to Pevent mal-nutrition or denciency. It is needed to instruct obese childrul to carry out interlsive w[rk-outs at least 3 times a weft through systematic grams. It is also needed that parents and teachers educate elementary school chil(hell from an early age with iDcimation on the frequency and interlsity of work-outs to prevent obesity. It is also desirable that children be eddcated to cauy out physical activities that address thetr specific needs. As a result of this study, obesif management must be started from elementary school and children should be instructed to act in hiRh-interlsity actirities when they spend leisure time.

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