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1
쥐의 말초신경손상모델로서의 궁둥신경절단모델과 L5 척수신경절단모델의 비교연구

송대용(Dae- Yong Song),이지혜(Ji-Hye Lee),유하늘(Ha-Nul Yu),박채리(Chae-Ri Park),우란숙(Ran-Soak Woo),홍성엽(Sung- Yaup Hong),전영희(Young-Hee Cheon),구형남(Hyung-Nam Goo),백태경(Tai-Kyoung Baik) 대한체질인류학회 2012 해부·생물인류학 (Anat Biol Anthropol) Vol.25 No.1
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  KCI
  
  DBpia
연구자들은 L5 척수신경만을 선택적으로 절단하고 L5 척수분절을 다른 척수 부위와 구별하여 연구함으로써 기존의 궁둥신경절단 모델의 단점을 보완할 수 있는 새로운 말초신경절단 모델을 제안하고자 이 연구를 수행하였다. 300?350g Sprague Dawley계 흰쥐를 이용하여 4개의 실험군으로 나누어 연구하였다. 1. 궁둥신경 절단군: 궁둥신경이 온종아리신경과 정강신경으로 나눠지는 오금부위에서 궁둥신경을 절단한 군. 2. L5 절단군: L5 척수신경이 L5 척수사이구멍을 통과하여 출현하는 부위에서 L5 척수신경만을 선택적으로 절단한 군. 3. 접합군: L5 척수신경 절단 후 바로 봉합한 군. 4. 대조군: 시술 과정은 동일하게 수행하였으나 신경에 손상을 가하지 않은 군. 그리고 일부 실험동물의 경우에는 L5 척수분절을 확인하기 위하여 L5 척수신경을 절단한 후 FluoroGold를 주입하였다. L4와 L6의 척수신경뿌리를 기준으로 L4~L6 척수분절을 절단하고,40 ㎛의 두께로 관상절편을 제작한 결과 평균 180개 정도의 조직절편을 얻을 수 있었다. 이 중, 약 50개의 절편에서 FluoroGold에 양성반응을 보이는 척 수운동신경세포가 관찰되어 L5 척수분절범위는 50개의 절편, 즉 2mm로 하였다. L5 척수분절은 Cresyl violet 염색에서도 형태적으로 L4 및 L6 척수분절과 구별할 수 있었다. 시술 2주 및 4주 후에 동물을 희생하여 L5 척수 운동경세포의 수를 계수한 결과, 대조군과 비교하여 L5 절단군에서 약 8%의 신경세포 수 감소가 확인되었다. 접합군과 궁둥신경 절단군은 대조군과 비교하여 유의할 만한 변화를 보이지 않았다. 본 연구를 통해 제시한 L5 척수신경절단 모델은 L5 척수운동신경세포에서 기원하는 거의 대부분의 축삭을 절단하고, L5 척수분절에만 국한하여 연구를 수행한다는 점에서 기존의 궁둥신경절단 모델을 기반으로 한 연구 방법에 비해 매우 신뢰도가 높은 연구방법이라 제안하는 바이다. The aim of this study was to propose new more reliable peripheral nerve transection model to overcome the defect of the traditional sciatic axotomy model by specifically transecting L5 spinal nerve just after emerging from the intervertebral foramen and confining analysis area to the L5 spinal segment. The adult male Sprague-Dawley rats, weighing 300?350 g at the time of surgery, were used for the experiments. Four different experimental groups were used. 1. Sciatic nerve transection (Sc-Tx) group: transect the sciatic nerve in the popliteal fossa where it divided into the common peroneal nerve and tibial nerve. 2. L5 spinal nerve transection (L5-Tx) group: L5 spinal nerve was specifically transected. 3. Suture (Su) group: L5 spinal nerve was transected and immediately sutured. 4. Control group: the same surgical procedure with L5 spinal nerve transection group was performed except for the excision of L5 spinal nerve. To distinguish L5 motoneurons from the other level ones, the animals were received the retrograde tracer, FluoroGold into the axotomized proximal nerve stump. Serial coronal frozen sections at 40 ㎛ thick through the L4 to L6 spinal segment was performed and the resultant total number of sections was about 180. Approximate serial 50 sections (approximately 2 mm) could be considered as the L5 segment based on the number of the fluorescent signals (above 20). L5 spinal segment could be differentiated from L4 and L6 segment based on their morphological characteristics under Cresyl violet stain. In L5-Tx group, at 2 and 4 weeks post-transection, the number of L5 spinal motoneurons was reduced by 8%. Meanwhile, Sc-Tx and Su groups showed no statistically notable changes. In this study, the authors could propose more reliable peripheral nerve axotomy model than the conventional sciatic nerve axotomy model by specifically transecting L5 spinal nerve and confining the investigating area within the L5 spinal segment.
2
작업장에서 취급하는 CMR물질의 용량반응평가 방법 비교

이경화 ( Kyung Hwa Lee ),최한영 ( Han Young Choi ),김치년 ( Chi Nyon Kim ),노영만 ( Young Man Roh ),최희진 ( Hee Jin Choi ),박채리 ( Chae Ri Park ) 한국산업보건학회 (구 한국산업위생학회) 2018 한국산업보건학회지 Vol.28 No.1
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 ScienceON
 
 KISS
Objectives:Currently, there is only limited knowledge regarding the hazard of low-level exposure to CMR materials in workplaces. To overcome this limitation, a reference concentration for workers (RfCw) from among the risk assessment tools proposed by the US EPA is widely used to set a provisional workplace exposure level (PWEL) for CMR materials for which there are no established Korea Occupational Exposure Limits (KOELs) or subjective chemicals for work environment measurements as regulated by Korea Ministry of Employment and Labor (KMOEL). A simple European calculator of derived no effect level (SECO-DNEL) as proposed by REACH can also be used in place of RfCw to set the PWEL for chemicals. This study was performed to test the acceptability of using SECO-DNEL as an alternative to RfCw when setting a PWEL for low-level exposures. Methods: The RfCw and DNEL for the five CMR materials of dinitrogen oxide, catechol, 2-phenoxy ethanol, carbitol, and carbon black were calculated using the dose-response assessments of the US EPA for RfCw and REACH guidance for SECO-DNEL, respectively. They were compared using paired t-tests to determine the statistical differences between them. Results: For the five chemicals, the RfCw were 2.53 ppm, 0.10 ppm, 1.73 ppm, 1.66 ppm, and 0.05 mg/㎥, respectively, while the SECO-DNEL were 2.01 ppm, 0.11 ppm, 1.83 ppm, 1.77 ppm, 0.14 mg/㎥, respectively. There was no statistically significant difference between RfCw and SECO-DNEL. Conclusions: This study suggests that the SECO-DNEL could be applied in place of RfCw to set a PWEL for low-level exposure to chemicals, especially CMR materials. To further ensure the reliability of SECO-DNEL as an alternative tool, more chemicals should be applied for calculation and comparison with RfCw.

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