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      • KCI등재

        일반 및 동물복지농장 돼지의 혈액 및 근육 내 스트레스 인자 비교

        이정은 ( Lee Jeong-eun ),박진룡 ( Park Jin-ryong ),강다래 ( Kang Da-rae ),김희은 ( Kim Hee-eun ),남기창 ( Nam Ki-chang ),심관섭 ( Shim Kwan-seob ) 한국유기농업학회 2020 韓國有機農業學會誌 Vol.28 No.4

        Intensive farming methods that do not guarantee animal welfare can induce stress in pigs. Stress, in turn, can reduce their disease resistance and influence their hormones and metabolites in such a manner that productivity is negatively affected. This study was conducted to compare the stress related factors and blood characteristics of pigs raised on conventional farms and those raised on animal welfare farms. We measured the levels of cortisol, epinephrine and norepinephrine, biochemical parameters in blood and glycogen, L-lactate and heat shock protein 70 (HSP70) in muscle, as physiological markers of indicating the stress in conventional farm pigs (Control, n=10) and animal welfare farm pigs (Welfare, n=10). We found that there was a significant difference in the albumin-globulin ratio (A/G ratio), as well as the albumin (ALB), blood urea nitrogen (BUN) and aspartate aminotransferase (AST) levels between the two farms. Epinephrine was significantly higher in conventional farm, while level of norepinephrine was higher in animal welfare farm. There was no significant difference in cortisol, which is known as a stress hormone, across the two groups of farms. Muscular glycogen content was significantly high in animal welfare farm pigs. While L-lactate tended to be low in the animal welfare farm pigs, the difference between them and the conventional farm cohorts was not significant. HSP70 showed high levels of expression in conventional farm. Thus, we suggested that blood parameter results showed a stress response in the livers of conventional farm, and that catecholamine hormones, glycogen, L-lactate and HSP70 can be used as physiological factors of assessing animal welfare.

      • KCI등재

        일반농장과 동물복지농장 닭의 혈액 내 gas, 생화학인자, 호르몬 농도 및 근육 내 스트레스 반응인자 비교

        김희은 ( Kim¸ Hee-eun ),박진룡 ( Park¸ Jin-ryong ),이정은 ( Lee¸ Jeong-eun ),강다래 ( Kang¸ Da-rae ),장애라 ( Jang¸ Ae-ra ),최호성 ( Choe¸ Ho-sung ),심관섭 ( Shim¸ Kwan-seob ) 한국유기농업학회 2021 韓國有機農業學會誌 Vol.29 No.3

        It is generally recognized that high stocking density can increase the risk of immune dysfunction and reduction in productivity cause of elevated stress in broiler farm. The domestic livestock industry is implementing a certification system for animal welfare and livestock farms to reduce the problem of dense breeding. This study compared broiler from the general farm and animal welfare farm to confirm the effectiveness of animal welfare farms against stress. Gas concentrations in a farm of general and animal welfare farms were analyzed, blood (n=10) of the 28-day-old broiler was collected, the partial pressure of the gas in the blood was analyzed using whole blood, and biochemical factors and hormones were analyzed using the serum. Glycogen and L-lactate are analyzed using ELISA kit on finely pulverized muscles (n=10). CO<sub>2</sub> and NH<sub>3</sub> levels in the farm were significantly higher in general farm. Partial carbon dioxide tension (PCO<sub>2</sub>) in blood was significantly higher and partial oxygen tension (PO<sub>2</sub>) was significantly lower in the general farm. Creatinine (CREA), glucose (GLU), aspartateaminotransferase (AST), and creatinekinase (CK) in the blood were significantly higher in the general farm, and total protein (TP) and globulin (GLOB) were significantly higher in animal welfare farm. Blood corticosterone and norepinephrine were significantly higher in general farm and epinephrine was significantly higher in animal welfare farm. These results imply that animal welfare farm provides a stress-reduced breeding environment.

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