전기충격에 의한 휴면잠란의 부화시험

박광의,성수일 한국잠사학회 1973 한국잠사곤충학회지 Vol.15 No.2

춘채 휴면잠종을 산란후 약 50일째에 전기충격 (electric Shock)을 줌으로서 부화의 가능성 여부를 예비실험한 결과 다음과 같은 결론을 얻었다. 1. 휴면란에 대한 전기충격은 배자의 발육을 촉진 시켰다. 2. 전기처리시간은 5초에서 비교적 부화성적이 좋았다. 3. 침산처리일로 부터 약 15∼22일 사이에 산발적으로 부화가 시작 되었다.

논평(論評) : 가잠란(家蠶卵)의 휴면기구(休眠機構)

박광의 한국잠사학회 1973 한국잠사곤충학회지 Vol.15 No.2

곤충의 휴면성에 관한 연구업적은 수 많은 학자들에 의하여 보고되었지만 모든 연구결과는 더욱 일관성 없는 혼란만을 초래하였다. 그것은 이 화성이란 생물학적 현상이 매우 복잡성을 내포하고 있음을 의미한다. 그러나 최근 물리화학의 급속한 발전으로 인하여 새로운 어푸로치 방법이 개발됨에 따라 생물의 신비성 이 착착 들어나고 있으니 이 화성의 현상도 불원한 장래에 보다 명확하게 구명될 것이다.

가잠육종의 최근의 진보

박광의 한국육종학회 1978 한국육종학회지 Vol.10 No.3

中性子 및 γ線에 依한 작잠의 突然變異 誘發

朴光義 서울대학교 1966 서울대학교 論文集 Vol.17 No.-

This work was carried out in order to induce the mutation by treating the pupa or the eggs of tussah silkworm with gamma ray and neutron. The results obtained are as follows: 1. Though the whole pupa treated with neutron(high ro‥ntgen) become moths, they have no ability to copulate each other. The only moths emerged from pupa treated with neutron, 4000r are able to lay all un-fertilized eggs, some of which have a hole on the surface and nothing of contents. 2. The non-diapause eggs are treated with neutron in Sping, but the hatching ratio is only 50-60 percent. the whole eggs treated with gamma ray are never hatched. 3. The sensitivity of the pupa to neutron is weaker than that of the eggs. 4. The hatching ratio is an indirect proportion to the gamma ray dose. 5. Author find out a new mutant which is excellent in the cocoon quality, so he will do the progeny test next year.

잡지계를 괴롭히는 내ㆍ외적인 특수성

박광의 세계평화교수협의회 1987 廣場 Vol.173 No.-

施設蠶業과 養蠶構造의 改善

朴光義 農科大學 農業開發硏究所 1981 農業開發硏究 報告書 Vol.2 No.1

家蠶 休眠卵의 RNA 合成과 Aspartic Acid의 役割

朴光義,成洙一 서울대학교 1973 서울대학교 論文集 Vol.23 No.-

The work was carried out to observe a change in free amino acids of the diapause eggs(Bombyx mori), entering or terminating diapause by either hydrochloric acid treatment or cold storage. Paper chromatography was applied by the authors to the analysis of amino acids. The results obtained may be summarized as follows. 1. In the pre-diapause eggs 24 hr to 48 hr after oviposition, a few amino acids such as threonine, arginine, alanine, valine, tryptophan, and proline increased in amount, while cystine was decreased. 2. When eggs were immersed in HCl 20 hr after oviposition, the contents of amino acids did not increase as much as the non-treated one. Especially no increase of alanine was remarkable. 3. During cold storage at 5℃ for 40 days, the content of cystine as well as aspartic acid gradually decreased, and further only cystine completely disappeared. When the chilled eggs were treated wth HCl, no amino acids showed changes within 24 hr after HCl treatment. 4. Unlike the result that most amino acids did not change qualitatively or quantitatively after the onset of diapause, the amount of cystine and aspartic acid was decreased remarkably.

桑田 土壤面被覆이 桑樹의 根系分布 土壤의 化學性에 미치는 影響

朴光駿,李沅周,朴光義 서울大學校 農科大學 1982 서울대농학연구지 Vol.7 No.2

Soil mulching effect on the leaf yield and root distribution of mulberry tree and change in chemical properties of the soil was studied by conducting field experiment in the low-cutting mulberry field. For comparison, plots of clean tillage and herbicide weed control were made. The mulching materials used in the experiment were straw, chaff and polyethylene film. The results are summarized as follows: 1. The amount of fine and medium roots at the center part of the spacing increased by mulching. Chaff mulching increased espcially the amount of medium roots. The mulching treatment promoted the distribution of fine and medium roots at the depth of 20cm from the surface in the plots of polyethylene film and straw mulching. 2. It was evident that the pH and the content of Ca, Mg and available phosphate of the soil were higher in the center of the row spacing than in the surroundings of the tree, whereas the content of organic matter and exchangeable K were evenly distributed over the row spacing. 3. The chaff and straw mulching increased Ca and Mg content, but decreased the content of available phosphate in the soil. 4. There were no significant differences of root distribution and chemical properties in soil in the intra-tree space regardless of the locations. 5. The rate of cold injury of shoot tip increased in all the mulching treatments and especially in the chaff mulching. 6. The three year average leaf yield ('80 autumn-'81 autumn) was higher in the polyethylene film mulching than in the control. There was a distinctive decrease of it in other treatments and especially in chaff mulching. 7. The polyethylene film mulching increased the soil temperature by 1.4℃ and the other treatments rather decreased it by 1℃, as compared to the control. 8. The soil moisture content in the mulching plots was higher than in the control plot. In particular, the chaff mulching increased soil moisture content by 3∼6% than the control plot.

5령일수별급상량(齡日數別給桑量)의 증감(增減)이 가잠실용형질(家蠶實用形質)에 미치는 영향(影響)

박광의 ( K. E. Park ),문재유 ( J. Y. Moon ),김주읍 ( C. U. Kim ) 한국잠사학회 1969 한국잠사곤충학회지 Vol.9 No.-

5령일수별급상량의 증감이 가잠 실용형질에 미치는 영향은 다음과 같다. 1. 5령 전기간 표준급상량을 급여한 것보다 5령일수별로 30%증가 급여한 것은 실용형질 중 1과수에만 영향하고 화용비율, 전견중, 견증량, 견증비율, 상견비율에는 영향하지 않는다. 즉, 급상량증가는 강건성과 사량증가에는 효과가 없다. 2. 5령 전기간 표준급상량을 급여한 것보다 5령일수별로 30% 감량 급여하는 것은 실용형질 중 가장 중요한 전견기, 견증중에 크게 영향하며 화용비율, 견증비율, 상견비율 1계수에는 영향하지 않는다. 즉, 5령 전기간 표준급상량을 급여한 것에 비하여 5령 전기간 감량급여 한 것은 전상량이 12%, 상증중이 17% 가벼운 것으로 가장 불량하며 5령일수별로 감량 급여한 것 중에 특히 5령 7일째 감량한 것은 전상중, 상증중이 각각 5% 가벼운 것으로 불량하였다. This study was carried ont to investigate the effect of feeding amount on the metric characters of silkworm at each day of 5th instar. The results obtained from the study are summarized as follows: 1. The increased amount of feeding did not affect on the metric characters except the character, number of cocoon per litre, by each day of 5th instar. 2. The decreased amount of feeding did affect the weight of cocoon, weight of cocoon layer and did not affect the pupation, best cocoon rate, cocoon layer rate and number of cocoon per litre by each day of 5th instar. Namely, the weight of cocoon or weight of cocoon layer was decreased by 12 to 17 per cent in decreasing amount of feeding through 5th instar. Weight of cocoon or cocoon layer decreased by 5 per cent in the decreasing amount of feeding at just 7th day of 5th instar.

잠란(蠶卵)의 반문수(斑紋數)와 타계량형질(他計量形質)과의 유전상관(遺傳相關)과 환경상관(環境相關)에 관(關)한 연구(硏究)

박광의 ( K. E. Park ) 한국잠사학회 1962 한국잠사곤충학회지 Vol.2 No.-

1961年 春期에 卵紋數와 各種 實用形質과의 表現, 遺傳 및 環境相關과 各形質의 遺傳力을 농촌진흥청 蠶業試驗場에서 蠶品種 各道 連絡試驗에 供試하는 8 品種에 對하여 調査하였으며 該 試驗場에 保存되어 있는 蠶品種中 2(5) 品種에 對하여는 相關, 系統別 卵紋數 또는 變異를 調査하여 다음과 같은 結果를 얻었다. (1) 卵紋數와 全繭重, 繭層重 및 纖度와는 高度의 貧表現相關과 貧遺傳相關을 나타냈으며 環境相關은 나타나지 않았다. (2) 卵紋數는 中國系統이 가장 많고 日本系統이 中間이고 歐洲系統이 最小였다. (3) 減蠶比率과 卵紋數와의 關係는 一定한 경향이 없다. (4) 繭層重과 系長의 遺傳力은 全繭重, 纖度 및 卵紋數의 것에 比해서 적었다. (5) 卵紋集團의 小多角形 斑紋數가 많은 品種일수록 卵紋의 絶對數가 많았다. (6) 卵紋數의 變異는 歐洲系統이 가장 크고 中國系統, 日本系統의 順으로 적은 것 같다. 全繭重, 繭層重 및 纖度는 卵紋數를 基準으로 하여 그것들을 선발하면 間接的으로 選拔 效果를 높일 수 있을 것이며 特히 卵時代에 選拔함으로서 飼育의 經費를 節約할 수 있을 것이다. This work was carried out to investigate the phenotypic, genotypic, and environmental correlations between the number of the egg markings and other metric characters, and their heritability, with eight varieties in Bombyx mori L. Five European varieties, ten Chinese varieties and ten Japanese varieties were used to know the average number of the egg markings and their variabilities for each strains. The results may be summarized as follows: 1) there were highly negatively phenotypic and genotypic correlations between the number of the egg markings and total cocoon weight, weight of cocoon layer, or denier, but not an environmental correlation. 2) The average number of the egg markings in Chinese strain was more than that in other strains. 3) The correlation between the motality and the number of egg markings seemed not to be found. 4) The heritability of the weight of cocoon layer and the bave length was less than that of total cocoon weight, denier and number of egg markings. 5) The more the number of the polyhedral particles in an egg marking group became, the more the total number of egg markings increased. 6) The variation of the number of the egg markings in European strain was larger than that in any other strains. 7) It is expected that the selection for the number of the egg markings may have genetic advances of total cocoon weight, weight of cocoon layer, or denier. Especially the selection for the number of the egg markings before rearing will save the silkworm rearing cost.