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        • 桑田 土壤面被覆이 桑樹의 根系分布 土壤의 化學性에 미치는 影響

          朴光駿,李沅周,朴光義 서울大學校 農科大學 1982 서울대농학연구지 Vol.7 No.2

          Soil mulching effect on the leaf yield and root distribution of mulberry tree and change in chemical properties of the soil was studied by conducting field experiment in the low-cutting mulberry field. For comparison, plots of clean tillage and herbicide weed control were made. The mulching materials used in the experiment were straw, chaff and polyethylene film. The results are summarized as follows: 1. The amount of fine and medium roots at the center part of the spacing increased by mulching. Chaff mulching increased espcially the amount of medium roots. The mulching treatment promoted the distribution of fine and medium roots at the depth of 20cm from the surface in the plots of polyethylene film and straw mulching. 2. It was evident that the pH and the content of Ca, Mg and available phosphate of the soil were higher in the center of the row spacing than in the surroundings of the tree, whereas the content of organic matter and exchangeable K were evenly distributed over the row spacing. 3. The chaff and straw mulching increased Ca and Mg content, but decreased the content of available phosphate in the soil. 4. There were no significant differences of root distribution and chemical properties in soil in the intra-tree space regardless of the locations. 5. The rate of cold injury of shoot tip increased in all the mulching treatments and especially in the chaff mulching. 6. The three year average leaf yield ('80 autumn-'81 autumn) was higher in the polyethylene film mulching than in the control. There was a distinctive decrease of it in other treatments and especially in chaff mulching. 7. The polyethylene film mulching increased the soil temperature by 1.4℃ and the other treatments rather decreased it by 1℃, as compared to the control. 8. The soil moisture content in the mulching plots was higher than in the control plot. In particular, the chaff mulching increased soil moisture content by 3∼6% than the control plot.

        • 가잠(家蠶) Virus에 관(關)한 연구(硏究) -저항성(抵抗性)에 관(關)한 기초조사(基礎調査)-

          박광의 ( K. E. Park ), 강석권 ( S. K. Kang ) 한국잠사학회 1969 한국잠사곤충학회지 Vol.9 No.-

          1. 본 조사는 현재 우리나라에 보존되어 있는 계통을 전부 수집하여 Virus병에 대한 저항성을 계통 별로 조사함으로써 잠견생산에 막대한 피해를 주는 Virus병에 대하여 저항성이 강한 계통을 선발하는 동시에 선발된 결과는 앞으로 저항성품종 육성자료로 삼고저 하였다. 2. 가잠의 핵형다각체 Virus병에 대하여 저항성이 강한 계통은 N4, N6, N48, E111로써 log ED 50의 값이 0.799~1.611 범위 내에 있으며 강한 계통으로서는 N10, C62, N70, C108이고 log ED 50의 값은 5.159~7.258 범위 내에 있다(표 4참조) 그리고 일본계통이 가장 강하여 log ED이 3.770이 3.770이고 중국계통의 log ED 50은 3.564로서 다음이고 가주계통의 log ED 50이 3.381로서 가장 강한 계통으로 나타났다. 감염율의 회귀방정식의 방향계수는 0.1~0.6범위로서 우리나라 보존계통의 저항성의 균일성이 비교적 작을 경향을 나타냈다. 계통별 저항성의 유전현상에 대한 해명과 품질육성을 위한 구체적인 응용방법에 관한 구명은 차후의 숙제로 남게 되었다. 3. 잠체의 수분 및 회분과 Virus병에 대한 저항성과는 상관관계가 없었고(표 8참조) 다만 감잠비율(보통 사 육법에 의하여 조사된 것)과는 고도의 상관관계가 있다. 즉 4면 기잠에서는 수분 및 회분과는 관계가 없었고 3면 기잠에서는 수분은 +0.326 회분은 +0.326으로서 고도의 유의성을 나타냈고 1면과 2면의 회분에서는 각각 +0.520과 +386으로서 고도의 유의성을 나타냈으나 수분에서는 유의성이 없었다(표7 참조). 4. 교배조간에 있어서는 기호 205가 모든 형질에 있어서 가장 우수하였다. 특히 204는 강건성이 매우 좋았으나 견질에 있어서 대조구보다 약간 떨어진다. 기호 212는 견질을 약간 떨어지고 감잠비율은 보통이나 수견량이 공시품종 중 가장 많았다(표 11). 5. 종합적으로 기술하면 Virus에 대한 저항성이 강한 상기 몇 계통은 강건성 품종육성을 위한 기초자료가 될 것이며 계속 여러 계통 외 특성을 조사하여 특성 보존을 위한 품종보존의 완벽을 기하여야겠다. 1. Objectives and Importance. Many silkworms have been damaged by nuclear polyhedrosis virus diseases thoughout the country every year causing a decease in cocoon production by approximately ten per cent per year. The damage caused by the infections virus has occured in spite of complete disinfection. In this respect, it is well known it is impossible, at the present time, to protect the silkworm from these virus infections through chemical and physical control methods. Therefore, this author has attempted to solve this urgent problem from the view point of heredity and breeding, discovering the different resistances and heritabilities among 120 stains collected from throughout the country, and selecting the ones with highest resistance for the basic materials in the silkworm breeding. 2. Results of work 1) The strains with strong resistance to the nuclear polyhedrosis virus diseases are N4, N15, N48, C55/ and E111, the log ED50 values of them vary between 0.799 and 1.611. The susceptible strains are N20/, C62/, N76/, N79/ and C108/, the log ED50 values of them vary between 5.159 and 7.258. (Reference Table 4) The Japanese strain with a log ED50, value of 3.770 is the strongest, followed by the Chinese strain with a log ED50 value of 3.564. The weakest is the European strain with a log ED50 value of 3.3381. The direction coefficient of the regression equation of the susceptibility varies between 0.1 and 0.6, the uniformness of the resistance of the preserved strains of this country is comparatively low. The hereditary henomena of the resistance of each strain and the conerete method of its application for silkworm breeding main the subjects for later studies. 2) The content of water and ash in silkworm has not been correlated with the capability for resistance to the virus diseases(Reference. Table 8), but it is very significantly correlated with mortality rate (in common reaning). In the case of the silkworms which have just completed the fourth moulting the content of water and ash is not related to the mortality rate. In the case of the silkworms which have just completed the third moulting, however, the water(+0.326) and ash (+0.362) registered a high significance. The ash content in the first (÷0.520) and second (÷0.386) moults is highly significant but water content in both cases is not significant (Reference Table 7). 3) The No. 205 strain proved to be the best in character among the various F1 hybrids. No. 204 was very good in strength but a little lower in cocoon character than the control. No. 212 was a little low in cocoon character and mortality was average, but the cocoon harvest was the best among all the varieties offered for (Reference Table 9). 4), In short, the above mentioned strains which are known to have strong resistance to the virus disease are expected to provide basic data for breeding strong varieties. It is proposed that continued research should be conducted on the characteristics of various strains for a satisfactory preservation of various characteristics research should be conducted on the characteristics of various strains for a satisfactory preservation of various characteristics.

        • 中性子 및 γ線에 依한 작잠의 突然變異 誘發

          朴光義 서울대학교 1966 서울대학교 論文集 Vol.17 No.-

          This work was carried out in order to induce the mutation by treating the pupa or the eggs of tussah silkworm with gamma ray and neutron. The results obtained are as follows: 1. Though the whole pupa treated with neutron(high ro‥ntgen) become moths, they have no ability to copulate each other. The only moths emerged from pupa treated with neutron, 4000r are able to lay all un-fertilized eggs, some of which have a hole on the surface and nothing of contents. 2. The non-diapause eggs are treated with neutron in Sping, but the hatching ratio is only 50-60 percent. the whole eggs treated with gamma ray are never hatched. 3. The sensitivity of the pupa to neutron is weaker than that of the eggs. 4. The hatching ratio is an indirect proportion to the gamma ray dose. 5. Author find out a new mutant which is excellent in the cocoon quality, so he will do the progeny test next year.

        • 잠란상(蠶卵上)의 반문(斑紋)의 유전(遺傳)에 대(對)하여 (2)

          박광의 ( Kwang Eui Park ) 한국잠사학회 1963 한국잠사곤충학회지 Vol.3 No.-

          1962~1963년에 걸쳐서 농촌진흥청 잠업시험장에 보존되어 있는 잠품종중 4품종에 대하여 란문의 유전현상을 조사한 결과는 다음과 같다. 1) 정역교배의 어느 경우라도 F1은 모체의 난문수와 그 형태를 닮는다. 2) F2에서는 언제나 열성인 강성반문이 나타난다. 3) F3에 이르러 비로써 3:1로 분리한다. 4) F1의 난문은 수정전인 모체의 일부에 의하여 나타난 결과이므로 모성유전과는 달리 위모성유전을 한다. This work was carried out to know the hereditary phenomenon of the egg markings with 4 races preserved at Sericultural Experiment Station from 1962 to 1963. The results are as follows: 1. When crosses (Fig. 1) were made between females showing reticulate egg marks and males showing collected egg markings, the F1 markings were all reticulate. From the reciprocal cross was made between the collected marking females and the reticulate marking males (Fig. 2), all the F1 progeny showed the collected egg markings. 2. In the F2 the expected phenotype for reticulate markings (recessive) was not expressed but the collected markings (dominance) were always appeared. 3. When each F2 moth was inbred, the usual 3:1 ratio was obtained. 4. The F1 egg markings as well as the spindle shape egg were not determined by its own genes but by the genes of its mother, because those were formed before fertilization. The results of such influences, when they can be identified, are called material effects. And such a phenomenon was called pseudomaternal inheritance by Tanaka. (1919)

        • 논평(論評) : 가잠란(家蠶卵)의 휴면기구(休眠機構)

          박광의 한국잠사학회 1973 한국잠사곤충학회지 Vol.15 No.2

          곤충의 휴면성에 관한 연구업적은 수 많은 학자들에 의하여 보고되었지만 모든 연구결과는 더욱 일관성 없는 혼란만을 초래하였다. 그것은 이 화성이란 생물학적 현상이 매우 복잡성을 내포하고 있음을 의미한다. 그러나 최근 물리화학의 급속한 발전으로 인하여 새로운 어푸로치 방법이 개발됨에 따라 생물의 신비성 이 착착 들어나고 있으니 이 화성의 현상도 불원한 장래에 보다 명확하게 구명될 것이다.

        • 家蠶 休眠卵의 RNA 合成과 Aspartic Acid의 役割

          朴光義,成洙一 서울대학교 1973 서울대학교 論文集 Vol.23 No.-

          The work was carried out to observe a change in free amino acids of the diapause eggs(Bombyx mori), entering or terminating diapause by either hydrochloric acid treatment or cold storage. Paper chromatography was applied by the authors to the analysis of amino acids. The results obtained may be summarized as follows. 1. In the pre-diapause eggs 24 hr to 48 hr after oviposition, a few amino acids such as threonine, arginine, alanine, valine, tryptophan, and proline increased in amount, while cystine was decreased. 2. When eggs were immersed in HCl 20 hr after oviposition, the contents of amino acids did not increase as much as the non-treated one. Especially no increase of alanine was remarkable. 3. During cold storage at 5℃ for 40 days, the content of cystine as well as aspartic acid gradually decreased, and further only cystine completely disappeared. When the chilled eggs were treated wth HCl, no amino acids showed changes within 24 hr after HCl treatment. 4. Unlike the result that most amino acids did not change qualitatively or quantitatively after the onset of diapause, the amount of cystine and aspartic acid was decreased remarkably.

        • 약제(藥劑)에 의한 가잠교미아(家蠶交尾蛾)의 할애효과(割愛效果)에 대(對)하여

          박광의 ( Kwang E. Park ), 마영일 ( Young Il Mah ), 황석조 ( Seok Jo Hwang ) 한국잠사학회 1984 한국잠사곤충학회지 Vol.26 No.2

          잠종제조시 많은 잠종제조 노력을 성력화하기 위하여 누에 교미나방이의 할애제를 선발하고져 시중에서 쉽게 구할 수 있는 몇 가지 약제를 서로 잘 배합하여 이의 할애효과를 실험한 결과는 다음과 같다. 1. 약제 처리별 경시적 할애비율에 있어서 TCTFE 처리구는 10분만에 완전히 할애되었고 그 다음은 TCT FE+acetone (87.5:12.5v/v) 배합구와 TCTFE+acetic acid (50:50v/v) 배합구가 약제 처리 후 25분에 완전히 할애되었다. 그 외 acetic acid 원액처리는 1시간 40분 후에 완전히 할애되었다. 2. 약제 처리별 산란 조사에 있어서는 TCTPE+acetone (87.5:12.5v/v) 구가 아구별 평균 산란수 및 아구별 평균 수정란수를 보아도 약제 처리 중에서 가장 성적이 좋았고 무처리구인 대조구에 비해서는 유의성이 인정되지 않았다. 그러나 TCTFE와 acetic acid 단일처리구는 대조구에 비해 산란수 및 수정란수 모두 대조구보다 적었으며 불산란아도 acetic acid구는 39아로서 가장 많아 acetic acid 단일처리시는 약해로 인하여 산란태율이 저하되는 것으로 사료된다. 3. 약제 처리에 의한 다음 세대에 어떠한 영향을 주는가를 알고자 약제 처리를 한 후 산란한 다음 세대잠란을 사육한 결과 전 처리구가 경과일수 21일 23시간으로 대조구와 같았으며 화용비율, 수견양, 단견중, 견층중 및 견층비율 등의 실용형질은 대조구와 같은 수준으로 유의성이 인정되지 않았다. 이상을 종합하여 보면 처리구중 TCTFE구는 누에 교미나방이의 할애제로서 효과는 인정되나 누에의 생리와 관련하여 볼 때 단용처리보다는 TCTFE와 acetone 등 적당한 다른 유기용제를 선발하여 이들을 적당량배합하므로서 할애효과를 더 높일 수 있다고 생각된다. To save the labour reguired for separation of copulated moth during egg production, some of the chemicals available at the market were on screen test for easy separation of copulated moth of silkworm. The obtained results are summarized as following. 1. In a separation ratio of copulated moth along with a treating time, TCTFE(Trichloro-trifluoroethane) completely separated the copulated moth in 10 minutes. The combinations of TCTFE plus Acetone (87.5:12.5V/V) and TCTFE plus Acetic acid (50:50V/V) take 25 minutes for the complete separation of copulated moth. Use of Acetic acid solution only makes 100% separation of copulated moth in one hour and foully minutes and non-treatment shows only 47.5% of separation in three and half hours. On the other hand, There is no statistical significance between TCTFE plus Acetone and control in the egg productivity. 2. The combination of TCTFE plus Acetone (87.5:12.5V/V) does not infuluence the egg productivity of the moth, showing 443 grains for an average number of egg per moth out of which 417 grains are for the number of fertilized eggs while control shows 452 grains for an avarage number of egg/moth and 428 grains for the number of fertilized eggs. However a sing1e use of TCTFE and Acetic acid shows less egg productivity and number of ferilized eggs per moth, respectively as compared to those of the control. In particular, a single use of acetic acid makes an increase of number of non-egg prodncible moth and it seems to be brought due to a chemical damage. 3. In a rearing test of the egg laid by the chemical treated moth, there are no differences among the treatments in all of the useful characters of the larvae; larval duration, survival rate, cocoon yield, single cocoon weight, single cocoon shell weight and cocoon shell ratio. In these regards, it is recognized that TCTFE can be practically used for the separation of copulated moth and the combination of TCTFE and Acetone promote its efiectiveness on the separation of copulated moth.

        • 인공사료(人工飼料)에 의한 잠품종(蠶品種) 선발효과(選拔效果)에 관한 연구(硏究)

          박광의 ( Kwang E. Park ), 강석권 ( Seok Kwon Kang ) 한국잠사학회 1981 한국잠사곤충학회지 Vol.23 No.1

          누에의 人工飼科는 物理的 또는 化學的으로 뽕잎과는 그 性質이 매우 다르기 때문에 고치 生産性에 있어서 뽕잎의 경우 보다 不良한 成績인데 이 欠點을 解決하기 위하여는 우선 人工飼科에 適合한 品種이 育成되어야 한다. 그러므로 本試驗에서는 人工飼科育에 의하여 優良系統을 選拔했을 때에 發生하는 現象을 究明코저 遂行하였으며 그 結果는 다음과 같다. 1. 암고치의 繭層重과 繭層比率과의 相關關係는 Sa와 Y에서는 없다. 2. 숫고치에서 Sa系統의 全繭重과 繭層比率과는 相關關係가 없다. 3. 人工飼科育에 있어서 日本種系統과 中國種系統은 각각 選拔效果가 特異하게 나타났다. 4. 桑葉育에서 人工飼科育으로 전환하는 第1世代에서는 Sa와 Y系統이 모두 매우 不良한 成績을 나타냈으나 第 2, 3世代에서 急激하게 증가하여 選拔效果는 매우 컸다. 5. 第4世代에서는 致死率이 매우 높았기 때문에 第5世代는 桑葉育으로 繼代 增殖하였다. 6. 第6世代에서 10世代까지 選拔效果는 계속 증가하였는데 繭層比率만은 例外이었다. It is necessary to improve new silkworm variety suitable for the artificial diet different from the mulberry leaves in physical and chemical natures. The silkworms fed on artificial diet show low productivity in general. So the effectiveness of selection for cocoon characters was studied when the silkworms were reared on the artificial diet for several generations. The results obtained are as fellows: 1. There is no correlation between the cocoon shell weight and the cocoon shell percentage in female cocoons of both Sa and Y strains but in male cocoons. 2. No correlation exists between the weight of a cocoon and a cocoon shell percentage of male cocoons in Sa strain. 3. There is a specific effect of the artificial diet on selection between Japanese and Chinese strains compared with the mulberry leave rearing. 4. The cocoon quality of first generation in artificial diet rearing was very inferier to that of previous generation in mulberry leave rearing in both Sa and Y strains, but the effect of selection rapidly increased in first and second generation. 5. Very high mortality was noted in 4th generation to the degree of hardness for successive generation. So the larvae of 5th generation were fed on mulberry leaves for successive generation. 6. From 6th generation to 10th generation, the effectiveness of selection for cocoon characters increased continuously except the cocoon shell perctneage.

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