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젖소에서 유성분 분석을 통한 영양상태 평가 및 건강관리에 관한 연구 III. 고능력우 위주의 대규모 목장에서 우유중 단백질과 요소태질소 수준이 수태율에 미치는 영향
문진산,주이석,장금찬,윤용덕,이보균,박용호,손창호,Moon, Jin-san,Joo, Yi-seok,Jang, Gum-chan,Yoon, Yong-dhuk,Lee, Bo-kyeun,Park, Young-ho,Son, Chang-ho 대한수의학회 2000 大韓獸醫學會誌 Vol.40 No.2
Milk urea nitrogen (MUN) determination is being used an indicator of the protein-energy balance in dairy herds. A faulty balance can be corrected to optimize milk production and animal health. This parameter is regarded as a potential tool to evaluate suboptimal feeding practices and reproductive disorders. Therefore, the purpose of this study was to investigate the response of milk composition by regular feeding analysis and to compared the relationship between MUN and milk protein(MP) and fertility at the insemination period in Holstein dairy cows. Total of 355 artificial insemination (AI) for 150 Holstein cows in the herd were used to examine the relationship between MUN and MP content and conception rate. The AI occured for the cows 50 to 150 day in milk, and MUN and MP concentration were determined using automated infrared procedures. The mean${\pm}$standard deviation of MUN and MP concentration in the herd were $15.6{\pm}2.1mg/dl$ and $3.23{\pm}0.38%$, respectively. MUN contents of bulk milk were increase by elevated crude protein intake. The conception rate was lower in the cows in which the level of MUN was lower than > 8.0mg/dl (10.0%) or > higher than 25mg/dl (15.4%) relative to the cows in MUN content of 12.0~17.9 mg/dl (36.7%) at the time of insemination. Also, lower MP than 3.0% or higher MP than 3.25% were associated with a lower conception rates. Consequently, MUN and MP analyses may be used serve as a monitoring tool of protein and energy nutritional balance to improve reproduction efficiency in Holstein dairy cows.
문진산,주이석,구복경,김종염,김덕원,박용호,한태욱,Moon, Jin-san,Joo, Yi-seok,Ku, Bok-gyeong,Kim, Jong-yeom,Kim, Duck-won,Park, Yong-ho,Hahn, Tae-wook 대한수의학회 1998 大韓獸醫學會誌 Vol.38 No.1
The effects of chitosan on mastitis in lactating holstein cows were evaluated. Fifty six cows with intramammary infection(IMI) from nine farms were selected and the cows were fed with diets which contained 15~20g chitosan per day for 5~7 days. The milk samples were obtained from cows at 7 days and 14 days after administration to determine effect of the curing of mastitis and the reduction of somatic cell counts(SCC). The average value of SCC levels in quarter milk from the cows administrated with chitosan significantly decreased up to 31.8% and 47.7% at 7 and 14 days, respectively(P<0.05). The cure rates of chitosan for Stapylococcus aureus, coagulase-negative Staphylococci, Streptococci spp, other gram positive bacteria and coliforms were 30.4, 42.8, 33.3, 66.6 and 54.5 % respectively. Twenty three out of 64 cases were cured by feeding with chitosan. The results showed that administration of chitosan could reduce SCC in milk and improve cure rates of bovine mastitis caused by microorganisms. The further studies will be pursued to study on the mechanism of chitosan in the immune responses of cows with mastitis.
젖소 유방염 유래 Staphylococcus aureus의 Coagulase Gene 유전형 분석에 의한 감염경로 규명
문진산,이애리,임숙경,주이석,강현미,김종만,김말남,Moon, Jin-San,Lee, Ae-Ri,Lym, Suk-Kyung,Joo, Yi-Seok,Kang, Hyun-Mi,Kim, Jong-Man,Kim, Mal-Nam 대한수의학회 2003 大韓獸醫學會誌 Vol.43 No.1
Because Staphylococcus aureus (S. aureus) has variable number of short sequence repeat region in coagulase gene, it has been used to investigate the relatedness of S. aureus isolates. In this study, we isolated S. aureus strains from 20 dairy farms with bovine mastitis from September 2000 to August 2001. PCR-RFLP analysis of coagulase gene revealed 10 different patterns. Most of the S. aureus isolates showed only one coagulase gene RFLP pattern per farm. However, there were several S. aureus clones spreading between dairy farms. All the farms showed poor management conditions of milking machine and milker, indicating that managements for mastitis control program include use of proper milking matching, premilking sanitation, and segregation in the S. aureus infection herd. Our data suggest that PCR-RFLP analysis of coagulase gene might be applicable for the epidemiological investigations of S. aureus isolated from bovine mastitis cows.
돼지에서 감염의 조기진단 지표로서의 Haptoglobin 적용에 관한 연구
문진산,남향미,구복경,주이석,정석찬,김종염,박용호,Moon, Jin-san,Nam, Hyang-mi,Ku, Bok-gyeong,Joo, Yi-seok,Jung, Suk-chan,Kim, Jong-yeom,Park, Yong-ho 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.3
Sera of pigs with clinically normal and infectious conditions were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) to demonstrate the specific changes in protein profile. In the sera from pigs with infection, haptoglobin with a 40KDa protein was found to be increased as compared to that of sera from normal pigs. As a rapid detection method for monitoring infections at large-scale farms, one of acute phase protein, haptoglobin, was selected to compare the concentrations between infectious and non-infectious conditions. Haptoglobin concentrations were low in pigs with clinically normal conditions but significantly increased in pigs with Aujesky's disease, hog cholera and parvo-virus infection. The studies provide that haptoglobin can be used as an indicator to monitor infections early at farm level.
돼지 오제스키바이러스 재조합단백질 gp50, gIII와 α-ADV을 이용한 Quil A-ISCOMs 접종시 백혈구아군 분포율에 관한 연구
문진산,박용호,정석찬,구복경,이성일,현방훈,안수환,Moon, Jin-san,Park, Yong-ho,Jung, Suk-chan,Ku, Bok-gyeong,Lee, Sung-il,Hyun, Bang-hun,An, Soo-hwan,Davis, W.C. 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.2
An effective candidate subunit vaccine was prepared by using the immunostimulating complexs(ISCOMs) with Quil A and recombinant protein(gp50, gIII and inactive $\alpha$-ADV) Aujeszky's disease virus(ADV). The weaned pigs were twice immunized with a ADV-ISCOMs, and followed by intramuscular challenge with $1{\times}10^4$ $TCID_{50}$ ADV(strain Yangsan). The unvaccinated pigs were also challenged with same dose of ADV. At 5 days after challenge, the control pigs have developed ADV clinical signs. Whereas, the vaccinated pigs protected them from ADV-induced acute symptoms and death. Also, to identify the lymphocyte subpopulation in peripheral blood with pigs from ADV-ISCOMs vaccinated and control group, lymphocyte reacted with a panel of monoclonal antibodies which are specific to swine leukocyte surface antigens and assayed by the flow cytometry. MHC class I, CD2, CD8, N cells, CD11a, and CD45 antigen positive cells were decreased after inoculating virulent ADV Yangsan strain in control group. The data indicated that ISCOMs technique was useful in ADV subunit vaccine preparation and demonstrated the importance of gp50, gIII as a component of ADV vaccine.
돼지 단독균 인공감염 및 항생제 치료에 따른 Haptoglobin치의 변화
문진산,남향미,구복경,주이석,정석찬,김종염,박용호,Moon, Jin-san,Nam, Hyang-mi,Ku, Bok-gyeong,Joo, Yi-seok,Jung, Suk-chan,Kim, Joang-yeom,Park, Yong-ho 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.3
The changes of concentration of serum haptoglobin, body temperature, hematological result and antibody titers were compared in pigs given intravenously injection of E rhusiopathiae and control pigs. Pigs administered with E rhusiopathiae injections showed acute septicemia and arthritis. Also, statistically significant changes observed in the total white cell counts and body temperature. An increased haptoglobin concentration in serum was detected after E rhusiopathiae infection, but not during antibiotics treatment. The results indicated that measurement of the concentration of serum haptoglobin may be useful in the early diagnosis of infectious disease, such as Erysipelas.
백혈구 표면항원 특이 단크론항체를 이용한 한우의 말초혈액 백혈구 아군에 관한 연구
문진산,박용호,정석찬,구복경,강병규,Moon, Jin-san,Park, Yong-ho,Jung, Suk-chan,Ku, Bok-gyeong,Kang, Byong-kyu 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.2
The proportion of leukocyte subpopulation in the host is of a great importance in understanding their functions and disease progress. Many methods have been developed to seperate leukocytes and to measure their activities. Characterization of immune cell subpopulations in Korean native cattle was performed using a set of monoclonal antibodies specific which are specific to bovine leukocyte differentiation antigen. Peripheral blood leukocytes from fifty Korean native and ten Holstein cattle were collected and analyzed for the investigation of leukocyte subpopulation by using monoclonal antibodies and flow cytometry. The result indicated that Korean native cattle have significantly higher proportion of leukocyte subpopulations expressing MHC class II molecules and BoCD4 than Holstein cattle.
젖소에서 유성분 분석을 통한 우군 건강관리프로그램의 개발
문진산,손창호,이보균,주이석,강현미,김종만,김병태,문현식,Moon, Jin-San,Son, Chang-Ho,Lee, Bo-Kyeun,Joo, Yi-Seok,Kang, Hyun-mi,Kim, Jong-Man,Kim, Byoung-Tae,Moon, Hyun-Sik 대한수의학회 2002 大韓獸醫學會誌 Vol.42 No.4
The purpose of this study was to develope a computer program to help with gross diagnosis of protein-energy balance and feeding management practice and with the prediction about the risk possibility of productive disease such as reproductive and metabolic disorders by evaluating fat, protein, and milk urea nitrogen (MUN) from individual cow milk in dairy herd Somatic cell counts also represent the condition of udder health. The principal flow charts of this program was to check on herd management, sampling the composite milk, analysis the milk composition, conversion of data from milking equipment to program, input and analysis of data in program, and report. This program is compatible with window 95/98 system. The major analytical elements of this program were presented as; the profile of herd lactation curve analysis of the test-day milk production level, the distribution of somatic cell count, the fat to protein ratio to evaluate body energy balance, and the interpretation of dietary protein-energy balance by milk protein and MUN contents for individual cows. This program using milk fat, protein, MUN, and somatic cell counts will serve as a monitoring tool for the protein-energy balance and the feeding management practice, and for distribution of mastitis in individual cows. It will also be used to manage the nutritional and reproductive disorders and mastitis at the farm level.
분만 후 첫 번째 번식검진시 난소 및 자궁 질환에 따른 유성분 수준 비교
문진산,신종봉,손창호,주이석,강현미,김종만,Moon, Jin-San,Shin, Chong-Bong,Son, Chang-Ho,Joo, Yi-Seok,Kang, Hyun-Mi,Kim, Jong-Man 대한수의학회 2002 大韓獸醫學會誌 Vol.42 No.2
The relationship between level of milk composition and conditions of ovary and uterus were analyzed in Holstein cows at seven farms participating in a reproductive herd health management program. Milk data were taken from 503 early lactating cows between 30 and 60 days in milk with reproductive examination with ultrasonography from september 1999 to August 2000. Milk fat, protein and solid-not-fat concentration in the herds were $3.70{\pm}1.08%$, $2.97{\pm}0.35$, and $8.41{\pm}0.61%$, respectively. The reproductive disorder relative to normal cows had higher risk in the cows that the level of protein was lower than 2.70%. Also, the higher milk fat than 4.50% were associated with a higher risks in the uterine disease and follicular cysts. Therefore, the cows with the fat to protein ratio of > 1.30 had higher risks for reproductive disorder such as cystic ovarian diseases, inactive ovaries and endometritis. These results indicated that cows diagnosed with reproductive disorder were energy deficient prior to reproductive disorder diagnosis. Consequently, milk fat and protein analyses may be used serve as a monitoring tool for condition of ovary and uterus in early lactating cows