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균일 기공 지지체 제조 및 기공 크기에 대한 세포 증식 효과
오명진,문승관,김성은,최성욱 한국생체재료학회 2013 생체재료학회지 Vol.17 No.3
Inverse opal scaffolds with different pore sizes were fabricated using uniform gelatin microspheres and used as a model system to validate the effects of pore size on cell proliferation. The poly(D,L-lactide-co-glycolide) inverse opal scaffolds exhibited well-arranged and uniform pore sizes (122, 293, and 462 μm). Two types of cells (NIH 3T3 and HepG2) were cultured on the inverse opal scaffolds with different pore sizes up to 28 days for proliferation assay. It was observed that NIH 3T3 cells proliferated faster in the scaffolds with pore sizes of 122 μm compared to the other pore sizes, which is attributed to the large surface area of the scaffold. In the other hands, HepG2 cells were found to favor pore size of 462 μm because they probably need enough exchange of nutrients and wastes for survival. These results reveal that the proliferation rate is affected by the pore size and each cell type has its own favorable pore size. The inverse opal scaffolds could potentially be used as a powerful platform for the studies on the optimization of pore size for tissue engineering and the interactions between cells or tissues and scaffolds.
최성욱,김중현,문승관,추지연,이혜원,박태준 한국고분자학회 2012 Macromolecular Research Vol.20 No.5
Small poly(D,L-lactide-co-glycolide) scaffold disks (2 mm in diameter) with a bimodal porous structure of 237.5±34.2 μm for large pores and 9.2±3.9 μm for micropores were prepared by gas-forming and controlled-precipitation methods. Chondrocyte-seeded scaffold disks were dispersed in an alginate solution containing culture media. The dispersion was transferred into a Teflon mold with a specific shape, eventually forming an alginate hydrogel (approximately 1 cm in dimension) that contains cell/disk aggregates after crosslinking using CaCl2. In vitro assessment of the alginate hydrogel showed significant increases in DNA and glycosaminoglycan content after 21 days, suggesting that chondrocyte still proliferated on the small scaffold disks in the alginate hydrogel despite its large dimensions. Moreover, in vivo immunohistochemical analysis of the alginate hydrogel demonstrated that chondrocytes secreted native extracellular matrix proteins (e.g., collagen, proteoglycan). We believe that our approach of using alginate hydrogel containing cell/disk aggregates with a shape fitted to the target site could provide a versatile platform to engineer large and thick tissues and/or organs.
Production of Uniform Emulsion Droplets Using a Simple Fluidic Device with a Peristaltic Pump
황석준,최성욱,문승관,김성은,김중현 한국고분자학회 2014 Macromolecular Research Vol.22 No.5
We fabricated a simple fluidic device consisting of a needle, a glass capillary, and a Tygon® tube to produceoil-in-water (O/W) emulsion droplets. In the fluidic device, the flow rate of the continuous phase was adjustedby a syringe or peristaltic pump, where a syringe pump was used for the discontinuous phase. We compared the performanceof the syringe and peristaltic pump for the continuous phase in terms of the average diameter and coefficientof variance of the resultant O/W emulsion droplets. It was found that uniform droplets could be obtained whenthe production frequency was well matched. In addition, the flow behavior of the discontinuous phase during droplet formationwas monitored using a high-speed camera, observing the back pressure in the peristaltic pump. By employmentof a peristaltic pump for the continuous phase, it was possible to prepare a large amount of emulsion droplets.
김주환,류태경,정기영,백동현,문승관,최성욱 한국약제학회 2015 Journal of Pharmaceutical Investigation Vol.45 No.2
Poly(D,L-lactide-co-glycolide) (PLGA) nanoparticleswere prepared by nanoprecipitation method usinga simple fluidic device with pristine and tapered glasscapillaries. An organic PLGA solution and an aqueouspoly(vinyl alcohol) solution were introduced into the fluidicdevice as the discontinuous and continuous phases,respectively. Five kinds of water-miscible solvents includingacetone, acetonitrile, dimethyl formamide, dimethylsulfoxide, and tetrahydrofuran were chosen as solvents forPLGA. This study investigated the effect of thermodynamicparameters such as diffusion coefficients (Dsw, Dws),exchange ratio (R = Dsw/Dws), and solvent–polymerinteraction parameter (v) on the size variation of PLGAnanoparticles. It was found that the size of PLGA nanoparticleswas inversely proportional to the exchange ratioand solvent–polymer interaction parameter. This variationin size might be attributed to the production of smaller localsupersaturation region in the case of lower exchange ratioand solvent–polymer interaction parameter. In addition, thesize of PLGA nanoparticles could be tuned by changing thepolymer concentration and flow rates in the fluidic device. This thermodynamic approach provides a rational basis forthe selection of solvent to control the nanoparticle size.
김지혜 ( Jihye Kim ),박현찬 ( Hyeonchan Park ),문승관,윤민석,김영휘,김연숙 ( Yeonsuk Kim ),최영미 ( Youngmee Choi ),노웅기 ( Woonggi Noh ),주문원 ( Moonwon Choo ) 한국정보처리학회 2011 한국정보처리학회 학술대회논문집 Vol.18 No.2
본 논문에서 소개하는 ‘행성’ 게임은 소셜 네트워크 접속자들이 주어진 공간과 자원으로 상품을 생산하거나 가공하고 교환함으로써 더 많은 상품을 생산해내는 물물교류 개념을 기본으로 한다. 새로운 행성에 정착한 인간들의 수렵과 채집, 육성, 가공, 건축을 위한 각자의 역할에 따른 생산과 소비, 그리고 상호간의 거래를 위한 의사소통 행위를 통하여 게임적인 재미와 동시에 경영과 유통과 관련된 경제활동의 기본적인 개념을 습득할 수 있게 하였다. 현재까지의 프로토타이핑 과정과 앞으로의 연구과제를 제시하고자 한다.