애완동물로부터 감염된 환자 및 애완동물에서 분리된 T. mentagrophytes 의 internal transcribed spacer 1 ( ITS1 ) 염기서열 분석

김정애(Jeong Aee Kim),임종현(Jong Hyun Rim),문상은(Sang Eun Moon),김규한(Kyu Han Kim),조광현(Kwang Hyun Cho) 대한피부과학회 2001 대한피부과학회지 Vol.39 No.10

N/A Background: Trichophyton mentagrophytes is a complex species and 3 perfect states, Arthroderma benhamiae, A. vanbreuseghemii, and A. simii have been described. In Korea and Japan, all of the isolates of T. mentagrophytes of which the perfect states determined were known as A. vanbreuseghemii till recently. However, in Japan, one isolate from a monkey was reported as A. simii and several strains from rabbits or patients were A. benhamiae. Therefore, it is necessary to confirm the existence of A. simii or A. benhamiae in Korea. Objectives: We performed this study to determine the teleomorphs of 19 strains of T. mentagrophytes isolated from Korean patients infected from pets and 4 from the skin lesions of rabbits. Metbods: DNA sequences of internal transcribed spacer 1 (ITS1) area of the 23 isolates and 3 reference strains were determined and matched with the data registered in GenBank nucleotide sequence database. Results: The sequencing data of 22 isolates were very similar to those of A. vanbreuseghemii and 1 to T, mentagrophytes var. interdigitale respectively. Conclusion '. Although all of the clinical isolates were related with A. vanbreuseghemii in this study, the possibility of the existence of A. simii and A. benhamiae in Korea is still high and further study is needed. (Korean J Dermatol 2001;39(8): 1086-1093)

피부 악성 종양의 통계적 고찰 ( 1990년 - 1995년 )

문상은 ( Sang Eun Moon ),조광현 ( Kwang Hyun Cho ),황지환 ( Ji Hwan Hwang ),김정애 ( Jeong Aee Kim ),윤재일 ( Jai Il Youn ) 대한피부과학회 1998 大韓皮膚科學會誌 Vol.36 No.1

Background: There is concem about the rising incidence of skin cancer. However, few articles have reported the change in the clinical and epidemiological aspects of skin cancer. Objective : We performed this study in order to clarifiy the recent changes in cutaneous premalignant and malignant lesions. Methods : A statistical analysis was made using 101 cases of cutaneous premalignant lesions and 205 cases of ma]ignant tumors out of a total number of 20,175 new outpatients that visited the Department of Dermatology at the Seoul National University Hospital during a 6 year period(1990-1995). Results : The frequency of premalignant lesions was 0.50% and that of malignant tumors was 1.02%. The frequency showed an increasing tendency compared to previous reports. In the premalignant lesion cases, actinic keratosis was most common and represented a figure of 82.2%. Among the malignant tumors, basal cell carcinoma was most frequent at 24.4%. Age, sex and anatomical site distribution of basal cell carcinoma, malignant melanoma was no different. The mean age of squamous cell carcinoma was greater than in previous reports and main preceding lesions were actinic keratosis and cheilitis. The frequency of lymphoma was markedly increased but the reason for this remains to be determined. Conclusion: Our findings showed the continous increasing incidence of skin cancers and emphasize the need for public eduacation, early diagnosis and proper treatment. (Korean J Dermatol 1998;36(1): 7-15)

비익부 기저세포암 수술 후 결손부의 Labial - alar Transposition Flap 복원

문상은 ( Sang Eun Moon ),김정애 ( Jeong Aee Kim ) 대한피부과학회 1995 大韓皮膚科學會誌 Vol.33 No.5

We report a case of nasal BCC in which the skin defect is reconstructed by a local labial-alar transposition flap. In nasal reconstruction, the local flap is the preferred methods. This flap has the advantage of a more acceptable scar, is easy to make, and less edematous than a superiorly based flap. However, for defects larger than 1cm, this flap is not advisable and the defect has to be located adjacent to th nsolabial fold. (Kor J Dermatol 1995;33(5): 855-858)

조갑하 사구 종양의 수술 치료

문상은 ( Sang Eun Moon ),원종현 ( Jong Hyun Won ),김정애 ( Jeong Aee Kim ) 대한피부과학회 2003 大韓皮膚科學會誌 Vol.41 No.5

N/A Background : Glomus tumor is a small encapsulated tumor that is derived from the subcutaneous glomus body. Treatment of subungual glomus tumor is the complete removal of the tumor. However, the surgical method is not familiar to us. Objective : The aim of this study is to introduce surgical treatment results of subungual glumus tumor in dermatologic surgery clinic of Seoul National University Hospital and Seoul City Boramae Hospital from 1995 to 2002. Method : We reviewed the medical records and checked clinical aspects, surgical methods and results. Results : All patients, 10, were female. Pain was elicited by contact in 80% and occurred spontaneously in 60%. Temperature hypersensitivity occurred in 50%. 90% of tumors were located in fingers. 70% of tumors revealed just blue or erythermatous discoloration of nail plate. Nail deformity was found in 30%. Post-operative mild distal nail splitting had developed in 2 cases. In 8 cases nails showed normal growth. Nobody complained of a recurrence of pain. Conclugion : Careful dissection and complete removal of the tumor almost offered permanent relief and it is thought that appropriate education of surgical skills should be needed and offered. (Korean J Dermatol 2003;41(5) . 559~563)

건선 환자의 조갑 병변에 대한 임상적 , 진균학적 관찰

김정애 ( Jeong Aee Kim ),문상은 ( Sang Eun Moon ),이동윤 ( Dong Youn Lee ),윤재일 ( Jai Il Youn ) 대한피부과학회 1996 大韓皮膚科學會誌 Vol.34 No.4

Background: Nail involvement in psoriasis is common. But their manifestions may often be difficult to differntiate clinically from onychomycosis, and results on fungal infections in psoriatic nails are still conflicting. Objectiye : The purpose of this study was to observe the clinical signs and mycolgical profiles of psoriatic nails. Methods : The frequency and types of clinical signs were observed prospectively according to sex, age, site, and accompaning tinea pedis. Repeated direct micrscopic and culture examinations, and KONCPA(KOH+Nail Clipping+PAS) study were done in the nails showing subungual kera tosis, and/or onycholysis. Results : Fifty nine(59%) out of 100 patients with psoriasis had psoriatic nail changes. Nail changes were more common in fingers than in toes(55% vs 34%), in males than in females(68.9 % vs 43.6%). The types of frequent nail changes were different in fingers and in toes. Dermatophytes were isolated in 2 cases(8%) and yeasts and non-dermatophytic moulds were isolated in 20-40% of psoriatic nails Conclusion : Psoriatic nail changes of fingers and toes showed different clinical signs. Thus we recommended psoriatic nails to be diagnosed according to the involved site. The isolation of dermatophytes in the psoriatic patients were not so rare. Repeated culture was thought to be the most valuable test because yeasts and moulds were frequently isolated as saprophytes or contaminants. (Kor J Dermatol 1996;34(4): 629-636)

3차원적 각질형성세포 배양법을 이용한 항진균제의 감수성 검사

김정애 ( Jeong Aee Kim ),이종희 ( Jong Hee Lee ),이동윤 ( Dong Youn Lee ),문상은 ( Sang Eun Moon ),조광현 ( Kwang Hyun Cho ) 대한피부과학회 2002 大韓皮膚科學會誌 Vol.40 No.4

N/A Background : Standardization of antifungal susceptibility testing for dermatophytes is important and several victimless such as inoculum size, length and temperature of incubation, media, and end point determination has recently been established. However, a more improved test model which can evaluate bioavailability and has clinical relevance is still needed. Objective : We performed antifungal susceptibility testing by using three dimensional keratinoctyte culture model, living skin equivalent (LSE), as an in vitro model. Methods : LSE was prepared and various concentrations of antifungals were added to media. Microconidia of Trichophyton mentagrophvtes was inoculated onto LSE and incubated for 6 days at 35℃. Results : Inhibition of fungal proliferation and in .won were observed at 0.1 ㎍/ml of terbinatine, 0.01㎍/ml of itraconazole solution, 0.l㎍/ml of itraconazole powder, and 10㎍/ml of fluconazole, respectively. Conclusion : Our culture model is similar to in vi o situation and the results were relatively well accordant to those of other previous studies. Our LSE model is considered t vi promising in vitro system for evaluating the activity and safety of antifungal agents. However, further study u ore various species of dermatophytes and n ore strains is still needed.

하수처리장 근로자에서의 족부백선

김정애(Jeong Aee Kim),정승용(Seung Yong Jung),문상은(Sang Eun Moon),권석운(Seog Woon Kwon) 대한피부과학회 1992 대한피부과학회지 Vol.30 No.1

Tinea pedis is frequently found in those people with poor hygine and in hot and humid environments. The authors investigated the clinical, epidemiologicol a id mycological characteristics of tinea pedis in 138 sewerage workers attending a sewerage plant in Seoul. Tinea pedis was found in 82, with a prevalence of 59.4%. The prevalence of tinea pedis increased with age and the period working at sewerage plant, however, there was no statistical significance. Also there was no difference in the prevalence of tinea pedis between the clerical workers and the field workers. Positive rate for KOH smear was 73.2%, and culture positive rates were 42.7%, producing 35 strains of dermatophytes. Twertyeight strains of Trichophyton Rubrum and 7 strains of Trichophyton mentagrophytes were isolated. Twentyseven yeast-form colonies were isolated, and Trichosporon beigelii was foungl in 19 samples. Most of the yeast forms were found mixed with dermatophytes and moulcis, However, 6 were isolated from direct smear positive cases and yieIded pure colonies of yeast. These included 4 cases of T. beigelii, 1 case of Candida parapsilosis, and 1 case of Candida hormicola. In view of the recent report of these fungi as pathogenic organism, these isolates, rspecially T. beigelii, were considered as a causative agent of tinea pedis in certain groups like sewerage workers. (Kor J Dermatol 1992; 30(1): 62-67)

3차원적으로 배양한 피부 섬유아세포에서의 교원질 합성에 미치는 Ascorbic Acid , TGF - β 및 IFN - γ의 영향

김정애 ( Jeong Aee Kim ),정진호 ( Jin Ho Chung ),문상은 ( Sang Eun Moon ),윤상호 ( Sang Ho Youn ),권오상 ( Oh Sang Kwon ),은희철 ( Hee Chul Eun ) 대한피부과학회 1996 大韓皮膚科學會誌 Vol.34 No.4

Background: The classical in vitro method for researching the fibroblast metabolism is a mono layer culture system. But there are a lot of facts that artificial influence of monolayer culture situations do not resemble the in vivo conditions. Recently three-dimensional culture method of fibroblast in collagen gel was developed and in this system fibroblasts seem to behave like in vivo situations. Objective : So we examined the effects of ascorbic acid. TGF-b, and IFN-r on fibroblast in collagen gel culture system and then compared these effects with those in monolayer culture system. We also studied the regulatory mechanism of collagen gene by ascorbic acid, TFG-b, and IFN-r by Northern blot analysis in monolayer culture system. Methods : Using fibroblasts of the neonatal foreskin, we measured the collagen synthesis after treatment of ascorbic acid, TGF-b, and IFN-r in three-dimensional culture and monolayer culture system. We aslo checked the change of mRNA of collagen after treatment in monolayer culture. Results : The relative levels of collagenase-digestible protein synthesis in the three-dimensional culture seemed to be lower than those in the monolayer system. However, difference of fold in crease was not statistically significant. The expression of collagen mRNA was increased after treatment of TGF-b and decreased after IFN-r treatment. Conclusion : From these results, fibroblasts in the three-dimensional culture system might resemble the in vivo conditions The molecular level of regulation of collagen synthesis by TGF-B. and IFN-r was thought to be transcriptional. The three-dimensional culture system could be a good model of culture system of fibroblast in studying of aging process or the role of cytokine in wound healing. (Kor J Dermatol 1996;34(4) : 608~615)

우췌양 및 건선양 임상양상을 보인 체부 백선

김정애,김석우,문상은 ( Jeong Aee Kim,Seok Woo Kim,Sang Eun Moon ) 대한피부과학회 1997 대한피부과학회지 Vol.35 No.6

A 34-year-old womar was admitted to the department of neurology, Seoul City Boramae Hospital for altered rnentality, food refusal and general weakness. She was a vagabond and had received anti-convulsant and anti-psychotic therapy over the past several years. She was referred to the department of dermatology for evaluation of skin lesions. There were light brownish, verrucous, hyperkeratotic plaques on the dorsum of both hands, wrists and soles, and pdoriasifom plaques on both elbows. Other skin lesions such as tina pedis, tinea crurir, and plaques on both elbows. Trichophyton ruhrum was isolat.ed from the dorsum of the hand, elbow, ingui nal fold, sole, and fingernail. On histopathologic examination, papillomatosis and hyperkeratosis were observed from the hand lesion and psoriasiforrn acanthosis from the elhow lesion. She was anergir to a Multitest CMI examination. When richophytin w;is injectecl intradermally, wheal de veloped after 20 minutes but induration did not developed untill 72 hours. We report this case because of its rare clinical manifestation and the interesting restilts of the immunologic test. (Korean J Derltlatol 1997;35(6): 1234-1240)

발진성 연모 낭종과 다발성 피지 낭종의 복합형태를 띠는 Hybrid Cyst

김석우 ( Seok Woo Kim ),문상은 ( Sang Eun Moon ),김정애 ( Jeong Aee Kim ) 대한피부과학회 1998 대한피부과학회지 Vol.36 No.1

Both eruptive vellus hair cyst and steatocystoma multiplex are uncommon conditions. There are clinical similarities between these two entities, but histological features are distinctive. We describe a patient with multiple subcutaneous nodules on the chest and both axilla, showing combined histological features of both an eruptive vellus hair cyst and steatocystoma multiplex. This case suggests that eruptive vellus hair cyst and steatocystoma multiplex are variants of one disorder which originate in the pilosebaceous duct. (Korean J Dermatol 1998;36(1): 116-119)