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강진만 연안에서 저인망에 어획되는 어류의 종조성 및 계절 변동
나해춘(Hae-Choon NA),이성훈(Sung-Hoon LEE),유태식(Tae-Sik YU),한경호(Kyeong-Ho HAN) 전남대학교 어업기술연구소 2019 어업기술연구소보고지 Vol.12 No.1
The fishes were caught by otter trawl from January to October in 2000 in coastal waters off Gangjin bay. A total of 3,327 fishes were sampled and classified into 69 species, 35 families, and 8 orders. Of the 8 orders, Perciformes, Scorpaeniformes, and Pleuronectiformes accounted for approximately 77.33% of the fishes in this area. Of 69 species identified, Leiognathus nuchalis, Synechogobius hasta, Tridentiger trigonocephalus, Cryptocentrus filifer, and Pholis fangi accounted for 35.65% of the fishes collected. The highest number of individuals was found in Autumn, while the lowest in winter. The richness index was the highest in spring(RI=10.320) and the lowest in winter(RI=5.093). The evenness index was the highest in winter(E=0.842) and the lowest in summer(E=0.798). The diversity index was the highest in spring(H’=3.410) and the lowest in winter(H’=2.865).
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사할린가자미(Limanda sakhalinensis)의 난발생 과정 및 자치어 형태발달
한경호 ( Kyeong-ho Han ),나해춘 ( Hae-choon Na ),박애전 ( Ae-jeon Park ),박재민 ( Jae-min Park ) 한국수산과학회 2017 한국수산과학회지 Vol.50 No.3
Egg development and morphological change of larvae of the Sakhalin sole Limanda sakhalinensis were studied by observing specimens obtained in a rearing experiment from fertilized eggs to the juvenile stage. The wild broodstock was collected in January 2010 and kept in a circular water tank (Ø1.5×1 m) at a temperature of 14.5±0.5℃. Fertil ized eggs ranged from 0.72 to 0.82 mm (0.77±0.07 mm, mean±SD) in diameter. The eggs were spherical, transparent and adhesive demersal. The egg yolk was divided from the oocyte 10 min after fertilization (AF), and an embryo was formed in 36 h AF. More than 50% of the eggs hatched within 133 h AF. The mouth and anus did not open until 3.5±0.25 mm total length (TL). At 4, days after hatching (AH), the fish became larvae 3.7 to 4.2 mm (4.0±0.36 mm TL), yolk absorption was completed and the mouth began to open. The left eye moved upward and the nostril moved to the right at 39 days AH. These post-larvae ranged from 8.0 to 9.9 mm TL (8.9±1.33 mm TL). At 50 days AH, the fish became juveniles (12.4±1.20 mm TL) There were 70-72 dorsal fin rays, 55-56 anal fin rays, 11 pectoral fin rays, and 6 ventral fin rays and the juveniles adopted a benthic life.
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Kang Min Kim(김강민),Hae Choon Na(나해춘),Jung Hee Park(박정희),Jae Seon Kang(강재선) 한국생명과학회 2009 생명과학회지 Vol.19 No.6
Bacillus polyfermenticus KJS-2 (B. polyfermenticus KJS-2)를 이용하여 발효된 콩(BP2FS)은 8가지의 병원성균에 대해 항균활성을 가졌다. BP2FS는 돌돔의 배양에 있어 사료첨가제로 사용되었다. 돌돔의 배양 시 BP2FS를 투여한 군(6×10⁴ cfu g?¹ 사료)과 투여하지 않은 군으로 나누었으며, 투여한 군은 120일 동안 하루에 두 번씩 투여 하였다. 120일 후에 투여한 군의 돌돔의 몸무게는 67.29±12.62 g이었으며 그렇지 않은 군의 몸무게는 56.56±8.21 g으로 나타났다. 생존율은 투여한 군(80%)과 투여하지 않은 군(40%)이었다. 폐사한 돌돔의 축적된 양은 투여한 군(18.95%)과 투여하지 않은 군(60.98%)이었다. BP2FS를 투여한 군에서 B. polyfermenticus KJS-2를 돌돔의 장내에서 검출한 결과 1.04×10⁴ cfu g?¹으로 나타났다. BP2FS를 투여 하지 않은 군의 돌돔에서는 iridovirus에 감염되었을 경우 나타나는 전형적인 증상인 비장과 간에서 비대세포가 관찰 되었다. 이 결과들은 BP2FS는 돌돔의 이리도바이러스에 대한 감염 시 예방효과를 가져온다 할 수 있다. Lyophilized powder (BP2FS) of soybeans fermented with Bacillus polyfermenticus KJS-2 (B. polyfermenticus KJS-2) exhibited in vitro antibacterial activities against eight pathogenic bacteria. BP2FS was used as a fodder additive for Oplegnathus fasciatus (O. fasciatus) culture. One group (UFD) of O. fasciatus was fed a commercial fodder, while another group (FD) was fed the same fodder, but including BP2FS (6×10⁴ cfu g?¹ fodder), two times daily for 120 days. The mean body weight of the FD group (67.29±12.62 g) was higher than that of the UFD group (56.56±8.21 g) after 120 days. The survival rate of FD was 80% compared to 40% for the UFD group. Cumulative mortalities in the FD and UFD groups were 18.95% and 60.98% respectively. B. polyfermenticus KJS-2 was isolated from the intestines of the FD group and the number of viable colonies was estimated to be 1.04×10⁴ cfu g?¹. Iridovirus and Vibrio vulnificus was detected in the organs of the UFD group but not in the FD group. All of the infected fish showed typical clinical symptoms of hemorrhage in their tail fins. Dissection of the infected internal organs revealed liver congestion and spleen enlargement - typical symptoms caused by iridovirus infection. These results clearly show that BP2FS is highly beneficial in preventing O. fasciatus from iridovirus infection.
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강담돔 (Oplegnathus punctatus)의 난 발생 및 자치어 형태발달
박재민 ( Jae Min Park ),이성훈 ( Sung Hun Lee ),윤성민 ( Seong Min Yun ),나해춘 ( Hae Choon Na ),한경호 ( Kyeong Ho Han ) 한국어류학회 2015 韓國魚類學會誌 Vol.27 No.2
강담돔의 수정난을 부화시켜 사육하면서 난 발생 과정과자치어 발달과정의 형태변화를 관찰하였다. 수정란은 구형의무색 투명한 분리 부성란이었고, 1개의 유구를 가지고 있었다. 난의 크기는 1.01~1.15 mm 범위였다 (평균 1.08±0.10 mm, n=20). 사육수온은 22.5~24.5°C (평균 23.0±0.05°C), 염분농도는 33.5~34.5‰ (평균 34.0±0.05‰)의 범위를 유지시켜 주었다. 난 발생 과정은 수정 25분 후에 난막과 난황이 분리되면서 배반이 형성되었다. 수정 후 55분에는 난할이 2개로 분리되어 2세포기에 달하였다. 수정 후 20시간 50분에는 배체가 머리 부분부터 난막을 뚫고 나오면서 부화하였다. 부화 직후의난황자어는 전장 2.10~2.20 mm (평균 2.15±0.07 mm, n=10) 로 입과 항문은 열려 있지 않았고, 난황과 유구는 부화 2일 후흡수되었다. 부화 후 12일째 중기자어는 전장 4.43~4.80 mm (평균 4.62±0.26 mm)로 꼬리지느러미는 부채꼴 모양으로 발달하였고, 흑색소포는 소화관 위쪽으로 침착하였다. Morphological changes in eggs and larvae of spotted knifejaw, Oplegnathus punctatus were observed by cultivation of fertilized eggs to juvenile stage. Fertilized egg was demersal and transparent. The egg has one oil glouble and its size ranged 1.01~1.15 mm (1.08±0.10 mm; mean±SD, n=20). Culture chambers were maintained 22.5~24.5°C in water temperature and 33.5 ~34.5ppt in salinity. It took 25 mins from fertilization to the blastoderm stage, 55 mins to 2 cell stage and 20 hrs 50 mins to hatching. Newly hatched yolksac larvae ranged 2.59~3.02 mm (2.81±0.25 mm, n=5) in total length, and mouth and anus were not still open. Yolk and oil globule were absorbed 2 days after hatching (DAH). Flexion larvae in 12 DAH ranged 3.02~3.07 mm TL (3.04±0.04 mm), and the caudal fin grew to fan-shaped, melanophores appeared on top of digestive canal.
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