신규 미백제 : Selina-4(14), 7(11)-dien-8-one

김청택 ( Cheong-taek Kim ),장윤희 ( Yun Hee Chang ),이상화 ( Sanghwa Lee ),강상진 ( Sang-jin Kang ),조완구 ( Wan-goo Cho ) 대한화장품학회 2005 대한화장품학회지 Vol.31 No.1

본 연구자들은 백출의 메탄올 추출물로부터 분리, 정제한 selina-4(14), 7(11)-dien-8-one (Selina)이 B16 멜라노마 세포에 대하여 매우 강력한 미백효과가 있음을 밝혀내었다. 본 연구에서는 Selina의 non-tumorigenic melanocyte cell line인 Melan-a 세포에 대한 작용 메카니즘에 대한 보고를 하고자 한다. 덧붙여 Selina를 함유하고 있는 제품의 임상효과도 연구하였다. Selina는 10 ㎍/mL의 농도에서 세포독성을 보이지 않으면서 Melan-a 세포의 멜라닌 합성을 50% 저해하였다. 또한 10 ㎍/mL의 Selina의 처리에 의하여 타이로시네이즈의 활성이 60% 감소시키지만 타이로시네이즈에 대한 직접적인 저해활성은 없는 것으로 밝혀졌다. 이러한 Selina의 작용 기작을 밝히기 위하여 연구자들은 타이로시네이즈, TRP-1, TRP-2의 3가지 유전자의 mRNA 수준과 단백질의 발현량을 RT-PCR과 western blotting을 이용하여 연구하였다. 그 결과 10 ㎍/mL의 Selina 처리에 의하여 타이로시네이즈의 mRNA와 단백질 양은 현저히 감소하였지만 TRP-1과 TRP-2의 mRNA 및 단백질 양에는 변화가 없었다. 이러한 결과는 Selina가 그 미백효과를 주로 타이로시네이즈의 발현조절에 의하여 나타낸다는 것을 알 수 있다. 0.2%의 Selina를 함유하는 화장품 제형을 이용, 7주간 20명의 피시험자를 대상으로 한 임상시험에서도 어떠한 부작용도 없이 통계적으로 유의한(p<0.05) 수준의 미백효과를 볼 수 있었다. 이상의 결과를 종합해 볼 때 Selina는 깨끗하고 밝은 피부를 위한 유용하면서도 안전한 신규 미백 원료임을 알 수 있다. We had previously reported that Selina (selina-4(14), 7(11)-dien-8-one) was isolated from methanol extract of Afractylodes rhizome and has strong whitening activity in B16 melanoma cells. In this report, we demonstrated its action mechanism in melan-a cells, non-tumorigenic melanocytes. We also investigated the clinical efficacy of cosmetic preparation containing Selina. Selina reduced the melanin synthesis of Melan-a cells by 50% at a concentration of 10 ㎍/mL without any apparent cytotoxicity. We also found that the treatment of cells with Selina decreased tyrosinase activity by 60% at a concentration of 10 ㎍/mL but Selina was not a direct inhibitor of tyrosinase activities. To elucidate the action mechanism of Selina, we investigated the changes in mRNA and protein level of tyrosinase, TRP-1 and TRP-2 using RT-PCR and western blotting, respectively. As a result, the mRNA and protein level of tyrosinase were markedly reduced at 10 ㎍/mL of Selina without any effect on TRP-1 and TRP-2. These results suggest that Selina exerts its whitening effect mainly through regulating expression of tyrosinase. A 7 week-clinical trial using formulation containing 0.2% selina-4(14), 7(11)-dien-8-one with 20 volunteers resulted in statistically significant whitening effect (p<0.05), without any adverse effect. Based on these results, Selina (selina-4(14), 7(11)-dien-8-one) can be s useful and safe ingredient for the cleanness and brightness of skin.

속수자의 멜라닌 생성 억제 물질

김청택(Cheong Taek Kim),정민환(Min Han Jung),김현식(Hyun Sik Kim),김호정(Ho Jeong Kim),강상진(Sang Jin Kang),강세훈(Se Hun Kang) 한국생약학회 2000 생약학회지 Vol.31 No.2

Two diterpenes and one new sucrose isovaleryl ester having inhibitory effects on melanogenesis in B16 mouse melanoma were isolated from Euphorbiae Lathyridis Semen which has been used in traditional medicine for cancer, tumors and warts. New sucrose isovaleryl ester was identified as α-D-giucopyranoside, 3,4,6-tris-O-(3-methyl-l-oxobutyl)-β-D-fructofuranosyl,2,6-bis(3-methylbutanoate) and two diterpenes were identified as ingenol-20-palmitate and 5,10-diacetyl-3-benzoyllathyrol(Euphorbia factor L₃) from their spectral data.

꾸지뽕나무 추출물의 피부 생리 활성

최학주,김청택,도민연,랑문정,Choi, Hak Joo,Kim, Cheong Taek,Do, Min Yeon,Rang, Moon Jeong 한국응용과학기술학회 2015 한국응용과학기술학회지 Vol.32 No.2

본 논문은 한국과 중국에서는 전통 한방약재로 오랫동안 사용된 꾸지뽕나무(Cudrania tricuspidata)의 잎, 줄기, 뿌리부분의 에탄올추출물의 물, 에탄올, 에칠아세테이트 용해성 분획물에 대한 피부 생리활성에 관한 실험결과이다. 대식세포인 macrophage(RAW 264.7 cell)에 대하여 이들 분획물들의 nitric oxide 및 염증성 사이토카인 분비 억제효과를 검토한 결과, nitric oxide 생성 억제에는 잎부위의 에칠아세테이트 분획물과 에탄올분획물이, 염증성 사이토카인인 $IL-1{\beta}$ 생성 억제에는 모든 분획물들이 효과가 있고, 염증성 사이토카인인 IL-6 생성 억제에는 잎, 줄기, 뿌리부위의 에칠아세테이트 분획물들이 효과가 있는 것으로 나타났다. 흑색종세포인 melanoma cell(B16-F10 cell)에서 멜라닌 생성억제효과를 측정한 결과, 잎의 에틸아세테이트 분획물 처리에서만 유의성 있는 억제효과를 나타내었다. 주름 개선 효과를 확인하기 위해 인체 정상 피부 섬유아세포( CCD986sk cell)에 대하여 콜라겐의 합성 촉진 효과를 측정한 결과. 잎, 줄기, 뿌리부위의 물 분획물과 잎, 줄기의 에탄올 분획물에서 콜라겐 합성 촉진 효과를 보였다. This paper has shown the experimental results about the physiological activities of water-, ethanol-, ethyl acetate-soluble fractions from ethanolic extracts of leaves, stems and roots of Cudrania tricuspidata on the skin, which has been used for a long time as a traditional herb medicine in Korea and China. The effects of these fractions on the secretion of nitric oxide and cytokines from macrophage(RAW 264.7 cell) exhibited that the ethyl acetate and water fractions from leaves inhibited the release of nitric oxide, all fractions inhibited thoses of inflammatory cytokine $IL-1{\alpha}$, and the ethyl acetate fractions of leaves, stems and roots inhibited thoses of inflammatory cytokine IL-6. The only ethylacetate fraction of leaves demonstrated significantly the reduction of melanin synthesis in melanoma cells. In order to evaluate the efficacy of collagen synthesis, the treatment with extracts on the human normal fibroblast cell(CCD-986sk cell) resulted in finding that the water fractions of leaves, stems and roots and the ethanol fractions of leaves and stems showed the increased synthesis of collagen.

RAW 264.7 세포에서 지모(知母) 80% 에탄올 추출물의 항염증 효과

이영근 ( Young Keun Lee ),김청택 ( Cheong Taek Kim ),최학주 ( Hak Joo Choi ) 대한본초학회 2017 大韓本草學會誌 Vol.32 No.3

Objective : According to recent studies, Anemarrhenae Rhizoma has anti-inflammatory activities of DW extract, but it hasn`t not yet conducted to evaluate inflammatory factors about 80% ethanol extract. Therefore, The aim of this study is to investigate the various effects of individual or combined 80% ethanol extract of Anemarrhenae Rhizoma on cell viability and various anti-inflammatory factors. Methods : Anemarrhenae Rhizoma extract was prepared with 80% ethanol. MTT assay, ELISA, and Luminex were performed in LPS-activated RAW 264.7 cell line to measure cytotoxicity, Nitric oxide (NO), cyclooxygenase-2 (COX-2), prostaglandin E2 (PGE₂), Leukotriene B4 (LTB₄), and cytokines (IL-1β, IL-6, and TNF-α), respectively. Results : At concentration of 200 ㎍/㎖ Anemarrhenae Rhizoma extract, cytotoxicity was observed in RAW 264.7 cells. However, at concentration less than 100 ㎍/㎖ of Anemarrhenae Rhizoma, cytotoxicity was not observed in RAW 264.7 cells. All concentration of Anemarrhenae Rhizoma extract showed no difference of NO, and IL-1β level in RAW 264.7 cells compared with control group. In contrast, at concentration of 100 ㎍/㎖ Anemarrhenae Rhizoma extract significantly inhibited LPS-induced production of COX-2, PGE₂, and LTB₄ level in RAW 264.7 cells. In addition, the production of proinflammatory cytokines (IL-6, TNF-α) in LPS-induced RAW 264.7 cells was significantly decreased at concentration of all or 10, and 100 ㎍/㎖, respectively. Conclusion : These findings demonstrate that Anemarrhenae Rhizoma has inhibitory effect on inflammatory mediators in LPS-activated RAW 264.7 cells showing possible developed as a raw material for new therapeutics to ease the symptoms related with inflammatory.

나草 에탄올추출물이 Human 유래 Jurkat 세포와 THP-1 세포의 알러지 및 염증 사이토카인에 미치는 영향

이영근 ( Young Keun Lee ),김청택 ( Cheong Taek Kim ),노성수 ( Seong Su Roh ),최학주 ( Hak Joo Choi ) 대한본초학회 2015 大韓本草學會誌 Vol.30 No.5

Objectives: The aim of this study is to investigate anti-inflammatory activity using various extracts of rice straw (DaoCao) extract (RS). Methods : To investigate the anti-inflammatory effect of RS, we examined the effect of RS on cytokines production on THP-1 cell. Cells were cultured in incubator (37℃, CO2 5%, 0.5% FBS-RPMI, 1X106 cells/ml). One hour after, Dermatophagoides pteronissinus (Dp., 10 ug/ml) was treated into cell and at 6 hour after, each different concentrations(0.1, 1 and 10 ug/ml) of RS were treated. The cells were incubated for 16 hours and harvest the supernatant. The levels of IL-4, IL-5, IL-6, IL-8, MCP-1 and TNF-αwere determined using a commercially available ELISA kit. Results: We investigated whether RS has the inhibition of inflammatory response in Jurkat cells and THP-1 cells. RS suppressed secretion of IL-4, IL-5, and TNF-α induced by house dust mites in Jurkat cells. It showed significant effects for all concentrations. RS suppressed the increased expression of IL-6, IL-8 and MCP-1 after treatment with mite in THP-1 cells. These results suggest that RS may be used as a valuable agent for treating allergic diseases such as atopy due to its anti-inflammatory property. Conclusions: RS showed significant biological activities with anti-inflammatory in the human T cells. These results suggest that RS may be used as a valuable agent for treating allergic diseases such as atopy due to its anti-inflammatory property. In terms of Korean traditional medicine, we expect the results to contribute to building of EBM (Evidence-Based Medicine).

꾸지뽕나무 추출물의 생리 활성(제1보)

최학주(Choi, Hak Joo),김청택(Kim, Cheong Taek),도민연(Do, Min Yeon),랑문정(Rang, Moon Jeong) 한국산학기술학회 2013 한국산학기술학회논문지 Vol.14 No.8

꾸지뽕나무는 한국과 중국에서 전통 한방약재로 오랫동안 사용되어 왔다. 본 논문은 꾸지뽕나무의 잎,줄기, 뿌리부분의 에탄올 추출물의 물, 에탄올, 에칠아세테이트 용해성 분획물에 대한 생리활성에 관한 실험결과이다. 이들 분획물들의 다양한 세포들의 성장에 대한 영향을 검토한 결과, 잎, 줄기, 뿌리의 에칠아세테이트 분획물이 macrophage(RAW 264.7 cell), melanoma cell(B16-F10 cell), fibroblast cell(CCD-986sk cell), lung carcinoma cell(A549 cell) 들의 성장을 현저하게 억제시키는 세포독성을 나타내었다. 자유라디칼 DPPH (di(phenyl)- (2,4,6-trinitrophenyl) iminoazanium)를 소거할 수 있는 분획물의 농도를 비교한 결과, 잎과 뿌리의 물분획물 그리고 뿌리의 에탄올분획물이 다른 분획물들에 비해 라디칼을 소거하는 항산화효과가 더 우수한 것으로 나타났다. Cudrania tricuspidata has been used for a long time as a traditional herb medicine in Korea nad China. This paper has shown the experimental results about the physiological activities of water-, ethanol-, ethyl acetate-soluble fractions from ethanolic extracts of leaves, stems and roots of Cudrania tricuspidata. The effects of these fractions on the growth of various cells have exhibited that the ethyl acetate fractions from leaves, stems and roots inhibited significantly the growths of macrophage(RAW 264.7 cell), melanoma cell(B16-F10 cell), fibroblast cell(CCD-986sk cell), and lung carcinoma cell(A549 cell). The water and ethanol fractions of leaves and ethanol fraction of stems demonstrated better antioxidant activities scavenging radicals than other fractions when compared with the concentrations of different fractions for scavenging free radical DPPH (di(phenyl)-(2,4,6-trinitrophenyl) iminoazanium).