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      • KCI등재

        차나무 경정배양시 생장조정제의 종류 및 농도가 품종간 신초 생장과 발근에 미치는 영향

        김주희(J.H. Kim),최형국(H.K. Choi),김정운(J.W. Kim),신길호(G.H. Sin),한재석(J.S. Han),김선우(S.W. Kim),오미정(M.J. Oh) 한국차학회 1998 한국차학회지 Vol.4 No.1

        Effects of growth regulators and their concentration on shoot growth and rooting of Tea stem cultures are as follow. Application of BA1.0~5.Omg/ℓ promoted shoot growth, leaf number, and callus induce in tea. It was not difference of shoot growth among cultivars, but C.V. Yabukita grew well in the application of BA. callus induced in all applitions, and treatment of BA1.0 -3.Omg/ℓ excellent. Number of root incressed in the applications of NAA, but not rooted in BA, thus root development achived by the treatment of IBA 1.0~3.Omg/ℓ . It was good for growing shoot growth in mixtured growth regulators, BA and GA₃then only a growth regulator.

      • KCI등재

        차나무 기내 배양을 위한 消毒 藥劑 選拔

        김주희(J.H, Kim),김정운(J.U, Kim),신길호(G.H, Shin),최형국(H.K, Choi),김선우(S.W, Kim) 한국차학회 1997 한국차학회지 Vol.3 No.2

        For cutting in vitro, culture conditions of tea plants, monthly contamination rate, selection of disinfectants and infectant exposure time, were investigated. Monthly contamination rates were 12% in May, 15% in Feburuary, 32% in November and 37% in September in vitro conditions. Therefore, it was considered that optimum cutting time was from March to April. The disinfectant of 95% ethanol and 7--8% CaOCl treated was heavily contaminated by 8~9% than 95% ethanol and 25% NaOCl treated by 3% but in the latter case, survival rate was reduced and the disinfective degree was reduced in low concentration. Contamination rates on disinfectant exposure time were 10~16% in the 95% ethanol and CaOCl treated for 15~20min and decreased by 9~11% in the 95% ethanol and 25% NaOCl for 15~20min. but necrosis rate of the latter was 18~79% and survival rate was reduced. The optimum disinfection conditions in vitro culture tea plants are as follows ; after dip plant in the 95% ethanol for 3~5 seconds, disinfect by 7~8% CaOCl treated for 15~20min. and rinse in sterilized water, 3~5 time repeatly.

      • KCI등재

        茶葉 收穫後 製茶前 經過時間 및 溫度가 製品의 品質에 미치는 影響

        김주희(J.H.Kim),박장현(J.H.Park),김정운(J.W.Kim),신길호(G.H.Sin),최형국(H.K.Choi),김선우(S.W.Kim) 한국차학회 1997 한국차학회지 Vol.3 No.1

        The effects of storage time and temperature of fresh leaves were investigated on the quality of made tea. The contents of total nitrogen tannin, chlorophylls, total amino acid and caffeine were decreased with increasing storage time and stroage temperature. Shape of green tea was better at high temperature in storage and long stroage time. Color of green tea extracts was better in short storage time from plucking to precessing of fresh tea leaves. When fresh tea leaves were stored for 9hr at 5˚C and for 3hr at 25˚C(room temperature), color of green tea extract, flavor and aroma were good. Optimum storage conditions of fresh tea leaves before processing were storage for 9hr at 5˚C or for 3hr at 25˚C.

      • KCI등재후보

        한국 재래종 차나무집단의 형태적 특성 및 성분조성에 관한 연구

        김주희(Kim. J.H),최정(J. CHOI),박용구(Y.G. Park) 한국차학회 2007 한국차학회지 Vol.13 No.3

        차나무 유전자원을 보존하기 위해 전남 26개 집단, 경남 12개 집단, 전북 5개 집단, 제주 3개 집단, 광주 1개 집단으로 전체 47개 집단을 선발하여 1992년부터 2004년까지 13년간 전라남도 농업기술원 차 시험장 시험포장에 식재하였다. 육성된 개체간의 형태적 특성과 내병성, 내한성 및 수형의 특성과 유효성분의 조성 등에 대해 개체간 조사를 실시하였다. 당년에 신장한 신아장의 길이는 11~15cm가 1,537(69%)주로 가장 많았다, 신아에서 발생된 엽수는 4~6매가 1,931(87%)주로 가장 많았다. 절간장의 길이 분포는 1-1.9cm가 1,278(58%)주로 가장 많다. 엽록소 함량은 41~80mg%가 1,815(82%)주로 대부분을 차지하였다. 엽장은 4.1~9cm가 2,129(98%)로 대부분이 잎이 적은 소엽종으로 구성되어 있었다. 엽폭은 2.1-3cm1,518(69%)주로 가장 많았었다. 엽 두께는 0.2-0.25cm 가 1,694(76%)주로 대부분이었다, 잎 끝(엽선단)은 둥글고 뽀쪽한 것이 1,037(47%)주로 가장 많았다. 엽면 주름은 1,763(80%)주가 적은 편으로 가장 많았다, 차엽의 광택은 1,908(86%)주가 많은 편이었고, 엽각도는 45도 보다 약간 처진 것이 1,662(75%)주로 가장 많았다. 측맥수는 잎당 9~10개가 1,163(53%)주로 가장 많았다, 나무 모양(樹姿)은 직립형이 1,174(53%)주 절반정도 나타났다. 내한성은 달관조사에서 2,206(99%)주가 약한 것으로 나타나 상록활엽수의 특징이 발현되었다. 탄저병은 달관 조사에서 대부분이 강한 편에 속하였다. . 암술 모양은 수술위로 돌출된 계통이 1,217(55%)주로 분포하고 있었다. 총 질소 함량은 4~5%의 차나무가 2,184(99%)주로 가장 많았다, 카페인 함량은 1.2~2%가 1,708(77%)주로 가장 많았다. 카테킨 함량은 7~11%가 2,072(94%)주였으며 가장 많은 것은 12.5%로 1주(0.1%)였었다. 총유리아미노산 함량은 1.1~2%가 1,446(65%), 2~3%가 763(34%)주였으며 비배관리가 부족한 상황에서의 자료로 분석되었고, 데아닌 함량 분포는 0.5~1%가 1,983(90%)주였으며, 비타민C 함량은 200-250mg/100g가 671(30%)주로 가장 많았음이 분석되었다. 조사 결과 엽형질 뿐만아니라 내병성, 내한성, 수형 등에서 개체간 차이가 뚜렷하게 나타났을 뿐만아니라 데아닌, 카데킨, 카페인, 유효 아미노산과 같은 유효성분 함량에서도 개체간 차이가 나타났다. 이것은 차나무 유전자보존을 위해 유전적인 변이가 다양한 개체들을 수집하여 보존포를 조성하는 것이 매우 의미가 있으며 필요한 사업으로 추정할 수가 있었다. For genetic conservation of tea populations, 47 populations were collected at Jeollanam 26, Gyeongnam 12, Jeollabuk 5, Jeju 3 and Gwangju 1 population and then the seedlings of those populations have grown on the tea filed Bosung Tea Experimental Station during 13th years from 1992 to 2004. The morphological characters, the tolerance of disease and cold resistant, and the variation of the contents in tea leaves were surveyed by checking the variation among populations. Among the morphological characters, the length of new shoot form 11 to 15 cm in this year have been represented 69%, i.e. 1,537 leaves, the number of leaves of new shoot from 4 to 6 was showed 87%, i.e. 1,931 leaves, the number of length of knot at from 1 to 1.9 cm show 58%, i.e. 1,278 leaves. In the characters of leaves, the leaf length from 4.1 to 9 cm was 98% i.e. 2,129 leaves, the leaf width from 2.1 to 3.0 cm was 69% i.e. 1,518 leaves, the thickness of leaf from 0.2 to 0.25 cm was 76% i.e. 1,694 leaves. Among the leaf form, the shape of the end of leaf was acuminate 47% i.e. 1,037 leaves, the leaf wrinkles was low 80% i.e. 1,763 leaves. The bright of leaf surface have high 86% i.e. 1,908 leaves and the contents of chlorophyll from 41 to 80 mg% was 82 % i.e. 1,815 leaves. The angle of leaf under the low 45 degree represented 75% i.e. 1,662 and the number of lateral vein from 9 to 10 was 53% i.e. 1,163 leaves. Elect type of tree shape show 53% i.e. 1,174 trees. The low cold tolerance shaw 99% i.e. 2,206 tree and the tolerance against Bacillus anthracis was representatived strongly by naked eyes. Shape of pistil in tea flower was upper divergence of stigma 55% i.e. 1,217 flowers. The total nitrogen from 4 to 5 % was 99% i.e. 2,184 leaves, caffeine from 1.2 to 2.0% was 77% i.e. 1,708 leaves, catechin from 7 to 11 % was 94% i.e. 2,072 leaves. Among them the highest contents was 12.5% only 1 tree. Total contents of free amino acid from 1.1 to 2.0% was 65% i.e. 1,446 trees, from 2.0 to 3.0 was 34% i.e. 763 trees. the contents of theanine from 0.5 to 1.0 % was 90% i.e. 1,983 trees and the contents of vitamin C from 200 to 250 mg/100g was 30% i.e. 671 trees. There are so much variation not only morphological characters but the content of caffeine, theanine, catechin and free amino acid among populations. Therefore, it is very important to conserve the genetic diversity of tea trees populations.

      • KCI등재

        보성지방 다원 토양내의 선충에 관한 연구

        김주희(J.H. Kim),박용구(Y.G. Park) 한국차학회 2000 한국차학회지 Vol.6 No.1

        In order to survey for nematodes in Posung tea plantation area, 45 sample sites were investigated. Meloidogyne species was observed at 35 among 45 sites, at the highest percentage 77.8%, Pratylenchus 11.1%, Parathylenchus 6.7% and Helicotylenchus 2.2%. The nematodes species were not identified excepted Pratylenchus fallax and Paratylenchus lepidus because the total samples number of nematodes was very a few. Meloldogyne were revealed higher frequency in the soil of older than of new tea plantation sites but Pratylenchus were observed also in new tea plantations. There are in general high frequency and various sorts of nematodes in old than in new tea plantations. The sorts and density of nematodes were different by the sort of soil for example the higher in clay than in loam. High variation of species and the a number of nematodes were in pH from 4.6 to 5.0 in soil. A number and density of nematodes were coincided with the soil conditions, not only the higher in 0.08~0.1 electro conductivity but in 51~80 g/kg organic contents. The contents of P₂O₅ with from 401 to 800 mg/kg in soil was affected the density and a number of nematodes for example the number of Meloidogyne was observed 18.1, Piatylenchus 5.8. Paratylenchus 37.5 and Helicotylenchus 21.5, while there was no evidenced at under 100 and over 800 mg/kg of P₂O5. The plain area of tea plantation was revealed more sorts and higher number of nematodes than over 15 degree of the planted area gradient. Those results are suggested that the nematodes are distributed prevailing in Korean tea plantation area. In order to conserved the tea plantation filed from the attacks of nematodes, there are more need research studies on the relationship of the density of nematodes and the production of tea leaves, the distributed situation, the identified the nematodes species, and the control methodologry against the nematodes in Korea.

      • KCI등재

        韓國 自生茶(Camellia sinensis L.) 花粉 貯藏 特性

        김정운(J.W. Kim),곽수년(S.N. Kwack),최형국(H.K. Choi),신길호(G.H. Shin),김주희(J.H. Kim),한재석(J.S. Han) 한국차학회 1999 한국차학회지 Vol.5 No.2

        For breeding new Korean tea cultivars, it is very important to get basic informations on pollen storage method. Results of this investigation are summarized as follows. The pollen germination rate during storage was changed a little differently among varieties, and pollens collected from flowers in bloom-the pollens collected in two days before bloom showed higher germination rate than them in one day after bloom or five days before bloom. During Storafe at 20˚C, the pollens germination rate was decreased in 3 to 5 days after storage, and pollens was not germinated in 25 days after storage. Optimum temperature for pollen storage was 0 to -5˚C. Pollens in one day after bloom showed low germination rate compared to them in bloom or in 1-5 days before bloom. In conclusion. to extend storage time of tea pollens, it should collect and store in bloom or one day before bloom.

      • KCI등재

        韓國 野生茶 遺傳資源의 品質 特性에 의한 品種群의 分類

        천종은(J.U. Chun),임준택(J.T. Lim),최정(J. Choi),김주희(J.H. Kim),임근철(K.C. Lim) 한국차학회 2003 한국차학회지 Vol.9 No.1

        The genetic relationship among Korean native tea tree germplasm based on chemical constituets is required to increase the efficiency of breeding. This work was done to classify 63 native tea tree lines by 7 chemical constituents which were analyzed by NIR system. The principal component and cluster analyses revealed 10 groups. The first group included 3lines that had the traits with lower total nitrogen contents, but higher vitamin C of leaves. The second group included 6 lines having lower free amino acid and theanine contents, but higher catechin contents of leaves. The third group included 2 lines having lower contents of free amino acid and theanine. the 5th group included 67%(42 lines) showing medium values of 7 chemical constituents. The 6th group included 2 lines that had higher caffeine and catechin contents, but lower fiber and vitamin C contents. The 7th group included higher caffeine and catechin contents, but lower theanine and vitamin C contents. The 8th group included lower catechin contents, but higher free amino acid, thenine and vitamin C contents. The 9th group included 2 lines showing lower caffeine and catechin contents, but higher theanine and fiber, and the last group included 1 lines that had lower catechin, but higher total nitrogen, free amino acid and thanine contents of leaves. The free amino acid contents that are the most important in flavor evaluation of green tea quality highly positively correlated with the contents of total nitrogen (0.848**), theanine(0.927**), but negatively with the contents of catechin (-0.501**) and fiber(-0.574**), respectively.

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