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정밀분사 시스템을 이용한 초고속조형 제작방식의 삼차원 PCL 및 PLGA 인공지지체 제작
김종영 ( Jong Young Kim ),윤준진 ( Jun Jin Yoon ),박의균 ( Eui Kyun Park ),김신윤 ( Shin Yoon Kim ),조동우 ( Dong Woo Cho ) 한국조직공학·재생의학회 2008 조직공학과 재생의학 Vol.5 No.3
The aim of this study is to fabricate scaffolds from two types of biodegradable materials using a precision deposition system that we developed. The precision deposition system, which is one of rapid prototyping(RP) methods, uses technology that enables the manufacture of three-dimensional microstructures. Fabrication of 3D tissue engineering scaffolds using the precision deposition system required the combination of several technologies, including motion control, thermal control, pneumatic control, computer-aided design and computer-aided manufacturing( CAD/CAM) software. Through the fabrication and human bone marrow mesenchymal stem cells(hBMSCs) proliferation experiments of two kinds of scaffolds using poly(ε-caprolactone)(PCL) and poly(lactic-co-glycolic acid)(PLGA), feasibility of application to the tissue engineering of precision deposition system is demonstrated.
자유 형상 제작 방식의 다축 적층 시스템 개발 및 쥐 모델을 이용한 PCL/PLGA/TCP 인공지지체의 골 형성 능력 평가
김종영(Jong Young Kim),심진형(Jin-Hyung Shim),김상헌(Sang-Heon Kim),이종원(Jong-Won Rhie),조동우(Dong-Woo Cho) 대한기계학회 2010 대한기계학회 춘추학술대회 Vol.2010 No.5
We investigated the validity of using SFF(solid free-form fabrication)-based scaffolds seeded with osteoblasts, and human umbilical vein endothelial cells (HUVECs) to enhance bone regeneration capacity. To accomplish this goal, SFF-based polycaprolactone (PCL)/poly-lactic-co-glycolic acid (PLGA)/tri-calcium phosphate (TCP) scaffolds were fabricated using a multi-head deposition system (MHDS). The blended PCL/PLGA/TCP scaffolds were seeded with osteoblasts and HUVECs and implanted into calvaria defect model in rats. At 8 and 12 weeks after implantation, micro-computed tomography (μ-CT), reverse transcription polymerase chain reaction (RT-PCR), and histological assays were conducted to know the effects of SFF-based scaffolds on osteogenesis. In vivo results indicated scaffolds in the osteoblast-HUVEC group had the largest area of new bone tissue. Therefore, we demonstrated through μ-CT and histological assays that scaffolds seeded with both human osteoblasts and HUVECs were superior to other groups.
다축 적층 시스템을 이용한 blended PCL/HA 인공지지체 제작
김종영(Jong Young Kim),이태진,심진형(Jin-Hyung Shim),김병수(Byung-Soo Kim),조동우(Dong-Woo Cho) 대한기계학회 2010 대한기계학회 춘추학술대회 Vol.2010 No.11
In this study, we manufactured polycaprolactone/hydroxyapatite (PCL/HA) scaffolds via a multi-head deposition system. The osteogenic potential of PCL/HA scaffolds was compared with that of PCL scaffolds fabricated with the same system. The fabricated scaffolds had a pore size of 400 μm and a porosity of 66.7% with fully-interconnected pore structure. The PCL/HA scaffolds had higher compressive strength and modulus than the PCL scaffolds. To compare the bone regeneration potential, two types of scaffolds (PCL and blended PCL/HA) were seeded with rat osteoblasts and implanted subcutaneously into thymes mice. After implantation for 8 weeks, the osteoblasts cultured on PCL/HA scaffolds expressed higher levels of osteopontin and osteonectin, both of which are osteogenic proteins. The PCL/HA scaffolds resulted in larger bone area and calcium deposition in the implants compared to the PCL scaffolds.
복합 조직 재생을 위한 자유형상제작기반 Multi-head tissue/organ building system 의 개발
심진형(Jin-Hyung Shim),이정섭(Jung-Seob Lee),김종영(Jong Young Kim),조동우(Dong-Woo Cho) 대한기계학회 2011 대한기계학회 춘추학술대회 Vol.2011 No.10
The aim of this study is to develop an advanced method for regeneration of three dimensional (3D) tissue or organ. The multi ?-head tissue/organ building system (MtoBS) which can deposit two or more biomolecules and various synthetic biomaterials was developed to regenerate heterogeneous tissue. The MtoBS possessing 6 heads was developed to build 3D pre-tissue in which structural, physiological, and anatomical factors were considered. Two heads are designed for synthetic biomaterials as a framework, another two are for cell dispensing, and the others are for protein dispensing. Feasibility of MtoBS for building 3D pre-tissue was shown by dispensing three different biomaterials into one structure. It was proved that position and volume control of two different hydrogel were controllable in the MtoBS. In conclusion, the MtoBS is expected to be a promising system for regeneration of heterogeneous tissue.
골 조직공학을 위한 자유형상제작 방식의 3차원 인공지지체 제작 및 In Vitro 특성 평가
심진형 ( Jin Hyung Shim ),이정섭 ( Jung Seob Lee ),김종영 ( Jong Young Kim ) 한국조직공학·재생의학회 2012 조직공학과 재생의학 Vol.9 No.1s
Tissue engineering is an emerging technique which has the potential to regenerate and repair damaged tissues or organs. In this paper, three-dimensional (3D) scaffold fabricated by solid freeform fabrication (SFF) technology and its mechanical property and cell adhesion characteristic were described. Polymer deposition system (PDS), which can dispense biodegradable polymers such as polycaprolactone (PCL) and poly (lactic-co-glycolic acid) (PLGA), was developed to fabricate a 3D scaffold for tissue engineering. In this study, PCL, PLGA, and blended PCL/PLGA were used as scaffolding materials. The dispensing conditions for each polymer were investigated using single-line test. Based on the result of single-line test, 3D scaffolds with fully interconnected 600 im pores were successfully fabricated by the PDS. Overall size of the scaffold was fixed at 25£ ̄10£ ̄4 mm which was targeted for application to spine regeneration. Effect of PCL, PLGA, and blended PCL/PLGA on compressive mechanical property of scaffolds was analyzed. In addition, in vitro cell interactions of scaffolds on MC3T3-E1 cells were evaluated using cell counting kit assay.