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김용옥,장기철,이철희,정한주,Kim Yong-Ok,Jang Gi-Chul,Lee Chul-Hee,Chung Han-Joo 한국연초학회 2005 한국연초학회지 Vol.27 No.2
This study was carried out to develop calibration equation of total volatile bases of tobacco leaf using near infrared spectroscopy(NIRS). Burley, imported flue-cured and oriental leaf tobacco samples were collected in 2005 crop year. Calibration equation was developed by modified partial least square method. The standard error of calibration and $R^2$ between traditional analytical method and NIRS analytical method were $0.038\%$, 0.983 for burley and $0.027\%$, 0.986 for imported flue-cured and oriental leaf, respectively. The standard error of performance and $R^2$ between traditional analytical method and NIRS analytical method were $0.048\%$, 0.940 for burley and $0.024\%$, 0.986 for imported flue-cured and oriental leaf, respectively. From these results, the NIRS analytical method seems to be applicable in analyzing total volatile bases of tobacco.
자생식물과 귀화식물의 수용 추출액이 Zoysiagrass의 발아와 유근생장에 미치는 영향
김용옥,장남기 한국잔디학회 1998 한국잔디학회지 Vol.12 No.3
The soil pH favored by Korean wild plants is 5.33~7.20, while naturalized invader species prefer soils of pH 3.95~6.48. The germination rate of Zoysia japonica was inhibited sharply, up to 60%, in extracts of naturalized plants of concentrations over 50%. Erigeron canadensis extract most strongly inhibited germination, while the Korean wild species, Cassia mimosoides var. nomame increased germination rates in concentrations of 30% and 50%. The seedling growth of Zoysia japonica in extracts of Korean wild species and naturalized species did not show differences, but was inhibited strongly, up to 60%, in the extract of Cassia mimosoides var. nomame concentration of over 15%. Analysis of the extract from Cassia mimosoides var. nomame identified procatechuic acid, $\rho$-hydroquinone, $\rho$-coumaric acid and ferulic acid at 254nm; and vanillic acid, hydroquinone, benzoic acid and cinnamic acid were discovered at 284nm. The seed germination and seedling growth of Zoysia japonica were investigated under different concentrations of phenolic compounds. Ferulic acid and vanillic acid were associated with an increased germination rate, while $\rho$-coumaric acid appeared to inhibit seedling growth. 자생종과 귀화종의 토양 pH를 측정한 결과 자생종의 토양 pH범위는 5.33~7.20인 반면 귀화 종의 토양 pH는 3.95~6.48로 자생종에 비하여 귀화종의 pH가 낮았다. 귀화식물 추출액 50% 농도 이상부터는 잔디의 발아율이 60% 미만으로 급격하게 억제되었으며 망초추출액이 가장 현저한 발아억제를 보인 반면, 자생식물인 차풀의 30%와 50% 추출액농도에서 발아율이 증가하였다. 자생식물과 귀화식물 추출액에 의한 잔디의 유근생장 억제는 각 농도에 따라 큰 차이를 보이지 않았지만 차풀의 경우는 15% 이상의 추출액 농도에서 유근생장이 60% 이상 현저하게 억제되었다. HPLC에 의한 차풀추출액의 성분 분석 결과는 254nm에서 protocatechuic acid, $ ho$-hydroquinone, $\rho$-coumaric acid, ferulic acid가 분석되었고 284nm에서는 vanillic acid, hydroquinone, benzoic acid, cinnamic acid가 분석되었다. Phenolic compound 각 농도로 잔디의 발아와 유근생장을 조사한 결과 ferulic acid와 vanillic acid는 잔디의 발아율을 증가시켰고 $\rho$-coumaric acid는 유근생장을 억제시켰다.