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최환석(Hwan-Suk Choi),김선암(Sun-Am Kim),신현재(Hyun-Jae Shin) 한국생물공학회 2015 KSBB Journal Vol.30 No.6
Insects are the most successful organisms on earth in terms of their diversity and adaptability. Insect biotechnology using this insect resource is an emerging area for future biotechnology with various applications. Insect resources have long been used to make food and/or functional food, feed, cosmetics as well as medicine and industrial ingredients. Recently, one of the most well-known industrial material from insect is spider silk that could be commercialize in near future. The insect cell lines have been used to express recombinant proteins that were difficult to be functional expression. For public purpose, while, the insect could be good amenity source and plant farming, so leisure resource. Only the interdisciplinary research will guarantee the successful story for insect biotechnology. And biochemical engineers should used insect as a bioresource for new products with applications in medicine, agriculture, and industrial biotechnology in near future. This review will cover state-of-the art of this field and the research and application areas of insect biotechnology and the possible role of biochemical engineer for the development of the future biotechnology using this bioresource.
김경문 ( Kyung Moon Kim ),김선암 ( Sun Am Kim ),김홍기 ( Hong Gi Kim ),오병준 ( Byoung Jun Oh ),윤제정 ( Je Jung Yun ) 조선대학교 공학기술연구원 2009 공학기술논문지 Vol.2 No.1
Biotech crops have been grown commercially for thirteen years since 1996. This paper describes current status and prospects of the biotech crops and the impact of plant biotechnology on the global agriculture from both economic and environmental perspectives. The 125 million hectares of biotech crops in 2008 by 25 countries represent 8% of the 1.5 billion hectares in the world. There have been net economic benefits at the farm level amounting to $44 billion for the thirteen-year period in both the developing countries and industrialized countries. During 1996-2007, the technology has accumulatively reduced agro-chemicals applying by 360 thousand tons and as a result, decreased the environmental impact associated with the use of agro-chemicals on these crops. In addition, deployment of biotech crops has also significantly reduced the release of 14.2 million tons of greenhouse gas emissions from cropping area. Biotech crops has been used as bioenergy crops for the production of biofuels and as bioreactors for the production of the food/feed ingredients in tile nutrient level as well as biopharmaceuticals. Biotech crops have been mostly issued in the public, thus, need for appropriate regulatory systems that are responsible and rigorous.
LC-MS/MS를 이용한 멀구슬, 협죽도, 황련 유래 활성성분의 벼 중 잔류양상 연구
박준성 ( Joon Seong Park ),남효송 ( Hyo Song Nam ),김용환 ( Yong Hwan Kim ),김도익 ( Do Ik Kim ),김선암 ( Sun Am Kim ) 한국환경농학회 2015 한국환경농학회지 Vol.34 No.2
BACKGROUND: Plant extracts have been used as environment friendly agricultural materials for organic farming in South Korea. However safety evaluation on the plant extracts was not properly tested. The aim of this study was to evaluate safety of the extracts from Melia azedarach, Nerium indicum and Coptis chinensis on cultivating rice. METHODS AND RESULTS: Pant extarcts 300-fold diluted were treated on rice, and residues of M. azedarach, N. indicum and C. chinensis were determined. The analytes from the rice samples were detected by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). The method was validated, and good linearities (r2=0.995-0.998), specificity, and recoveries were obtained. Limits of detection were 0.01 mg/kg for all of the target compounds. Recoveries were 79.3-118.3% at 0.1 mg/kg and 75.2-111.5% at 0.5 mg/kg. The residue levels were below 0.030 mg/kg for azadirachtin, 0.320 mg/kg for oleandrin and 1.460 mg/kg for berberine. CONCLUSION(S): The extracts of M. azedarach, N. indicum and C. chinensis contained azadirachtin, oleandrin and berberine as an active ingredient, respectively. The residue of three active ingredients dramatically decreased after treatment in all fruits, stems and roots of rice.